CRIMIC CR71821

Gene Prestin

CG5485

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:17898889..17907812	75A8-75A8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGACGCTACTTACCAATAGAGAAC	ACAAATTGCACTAAATCAATGCACTTC

Images

Construct Design Sequences

sgRNA sequence	CCCAAAAGACATAGAAGTAGTGG
sgRNA2 sequence	GGCTCTCTGCCCCAGTTAACTGG

left_200bp	right_200bp
ATTAGTACCCATTGGCTATAACGAAGAGATACCATCAAAAAAAGCTACTGGAACAATACCCAAGACACCCAAAGACGCTTTTCCGAATCCAAGTTCCCCAGTTTCTCGCTTCAGGCAGTCGCAATTCCAACGCGACCTACGCATTCATCCCTTTACAGACACCAATGTGGAACGCGGGACGAGCCCAAAAGACATAGAAG	AACTGGGGCAGAGAGCCAAACCACGTTTTGTGATATGTCCAAGTGATAGCTTGTGAATCGTAGCATCAAAACCCCTTCCTAGTTGCTAAAATCTATTTTAAACTGAATAGAAACAATGTCGCCGTCTACAATCAAGCAGAATAGGCTAAGTTTGTTTAAGTGCCTCCAAAATATTGATTTTTGTACTTAAAGCATACACA