CRIMIC CR71827

Gene CG33124

CG33124

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:2206525..2209102	22D4-22D4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTTAGAGCTTCTATTGCTGTGAC	TTACCGGTTCCATGGATCGCCTAT

Images

Construct Design Sequences

sgRNA sequence	AAAATGTCAGCTAAATTATGTGG
sgRNA2 sequence	GTTTATAAAAGTAATGAATGTGG

left_200bp	right_200bp
AATTGAATTTGTTGACCAAATATTTGCCAGTGATTTAATGATTTCATTTGGGTCTAGAGGTGTTTAGATCTGGAACATTTTATAGACTCATAAAATTGTTGCTATAACCACACTATAGTCGTTCTATAGTGTTTGCCCCAGATAACATATCTAAGACAATTCAAATTTCAGCAATCTCGGCTCCATTGGGCCATCCACAT	ATGTGGAAAACGTGTGTTCCATAAAATATTCCGCTGACCTAATTTATGTGACACTTTCGATTTCAAACATAAAACGCTATAATTCTATTTATAGATTTAAAAGAAACCTATACAATACGTAAAAAATGTTGTTCTTTTCTCAAGATTTTAATGAAAATTCTAGTAGTTTACCATCACCAGACTTTTTCTCGAGGGCATTG