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CR00955

CRIMIC CR00955

Gene Lac

CG12369

Information

Donor Location Cytology FBti Stock number
pM37-p1:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
TAAAAGGAAGTCACAGGCGGGTTGGT GCTATGCGAGGCAAACAAAAAACCGG

Images

Construct Design Sequences

sgRNA sequence AGAACTCGGCGGTTCATATAGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CACATTGTTGACGTGCTGCA CCGCCGAGTTCTGAAAGTCT AGGGACAGTCATGCTAGAACC GATGCTGCGTCCATCTCTGA
 
Amplicon 5' Amplicon 3'
CACATTGTTGACGTGCTGCATTAACATTCATAATTTCCACATGGCAACGTTCGGTTCATGGAGTCAGTTGACATTTGAAGGGCCCTGCAAGAGAACGAGATAGACAGGCTTTTCCTGAATTTTCCTTAGACTTTCCGCCGGAGTTGCTGGCCAAATTGCAGGAAATTGAATGGGGAAAGAACAAATATGGCCGCGGGCCACGACTTGCCGCGGAAAGGGAAAGCGCCTTGCATCAAATGTTATTTTGGCTAGTTGTTGTTGTTGCCCGTCTCACACGTTTTCCCGCTCGGCGGGTACAAAAAAGGGGGTTTGAAAAAAAAAAACAGCGTCTGGCCCTGTGATTGGTGCGGATATTTTTTGCATGCCACTCAATTTCCCTTTTTCCAGCCCTTTTGGGTGGTTGTTTCTGTCACCTCACAAAATGGCGCTCAACGGCGGGGTTTTATTTTGTCGCTTTAAGTAGTTTATGAAACATTAGAAACGGTAGCAGAACCAGCAACCCTTTCCACCATCCCTACTTGTCACCAATTTTCCAAGCGAGCGCTCATTGAAAGTCATCCAAGCAGGAAGCCAGGAGACCGAGAGACTTTCAGAACTCGGCGG AGGGACAGTCATGCTAGAACCTACACCTACACCTGTTCCGTGCATTTTCCATTTTCCATTTTCCGTTTTCTGTGCAGCGTCTCAGCGAAGTTGCATCCTTTTAGGACCTATCTACCTGCACTTCTCACATTTTTCCCTGACCAAAAAAAACTGAATTCTCACCTCTAGCTTTGCACAATTTTCCATTTCTTTGGCTCTTTTTTCGTTGAGCCTTCAACTGACAATTTATCTCTGGTTGTTTGTCCAGGGAGCAGAGGGATCCGGAGTCGGCAGTCGGGGTTCCAGAAACAAGGGGTGTTGCGTTAGCAACGTTGTTTTCATTTCGCATGATGGCAGGCGGAGACTTCCAGACAGGTTTCCCTGAAGGAAGGGCCTAAAACAAATGTGGACCCGCCCGCCTGCCAAGTCGGCGTTTTCATTACCTTTTGAGTTTCCATCTTTCCATCTTTCCTGTTTCTGTGCCGTTGCTGTTTCTGTTTTCCTTTCATTAGTTGTCAGAGATTGCCGAGTCAGAGATGGACGCAGCATC
 
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