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CRIMIC CR01560

Gene RhoGEF2

CG9635

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R :17044653..17027190	53E4-53F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	AAGATTCCACGATCTCCTTCGTTGGC	ATTCCGTCCTACTGGAGCGAAACCTT

Images

Construct Design Sequences

sgRNA sequence	ACCAGGATGCGACCGGAACAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAATGCGAGCCACAACAAC	GAGCCGCGTTTACCCAAGAA	GGAATCAGAGGAGCCTTCGG	CGGAAGAGCAGGTGGTCATT

 

Amplicon 5'

Amplicon 3'

ACAATGCGAGCCACAACAACGGCAGCATAATGGGCGGAGGCGTGCAATTGAATCAATCGGACAACTCCAACCCCGTTCTCCAGGCGCCGGGGGAGCGCAGTAGCCTCAATCTGACTCCCCTCTCCCGGGATCTGAGTGGTGGCCACACCCAGGAGTCCACGACGCCAGCGACCACGCCGAGTACGCCAAGCCTAGCATTACCAAAAAATTTTCAGTATTTAACCCTGACTGTGCGAAAAGATAGCAACGGATACGGGATGAAGGTATGTGCTTATACATTCAAAGTGCAACCCTTTATAATGCTTTCTTTGTTATGGATATTTAGAATTTAACGTAGAGCATTAAAAACTACTCCATTCCTCAACGAGCTTAACACGTTAGAATTTACTGGCAATAAAAAGTGTCATATACAATAGCTTAGTTGTAGGCTTTAACCTTTAGCTAAGGCAACAAATGTCTGTTACTGCCGACTCTAAAATGTCATTATAATAATTGATTTAACATTTAAGGGTGTTCTCTTTTAAACTTTTAAATTTAGTGTTGTTAAAAACAGCTGACATTAAAATAACATATTTTTACATTTATCTAAATCATTTACAGGTTTCCGGAGATAATCCTGTGTTTGTGGAGAGCGTTAAACCCGGAGGCGCAGCGGAGATTGCAGGCCTGGTTGCTGGCGATATGATACTAAGGGTGAGTAGGATAAATTCTCCTATTGCCCCAAATTCTTATATTCTTGACTACTCATTTTTAGGTAAACGGCCATGAAGTGCGACTAGAGAAGCATCCAACTGTAGTGGGTCTTATAAAAGGTATGAAAACATAGTCCTTTCAAGAAAGCAACCAAATTTATATTGTCTAGATGATGGTACAATAAATTTGTTCAATGGTCTGAGGAGATTTGGTTCAGGGGATTTCTAGGTAGCGGTTTCTCGAGGCGTCTTTCGGCACTGTTTGCACGATTTGATGGGCTCCGAGCGGGCTTCCGACTGGGGACCCTCTCCACATCTACACTTGGCTTCACTGCTCGGTATTCTGGGGCACTTGCGTTTGTTGTCCTTCCAATTCTCCGCCTGACGTTGCTTGCACTTGGCGCACTCGCACGGTGGCTTCTTGGGTAAACGCGGCTC

GGAATCAGAGGAGCCTTCGGACAGTTCTTGTCTTTGCAATGCGGTGGCACCAGTCCGGGAGGTCTGACCAGAGGAGTCTCGTCCAGCTGTCCCTTGGATATCGTTATTTCTTTCTTCTTAGACTGGCTCTCCTTAATGTACTTTTGAATGGTTTCGTTAAAAGCGTTGACATAGCTTGGTACTTTCTCCAAAAACGCAGCTAGTCCAGCGGGAAAGACGCTTAGTAAGTCGAAAACCGATTTAACTAATTTGTTGTAGTAGTAGATGCCAAAGAAGGACCATTCTCCCTCCGGTGGCGGCCTAGGCGGGCATATATTTAGTTTACGTCGCGCCTGATCGGCGTAGGGTTCGATGCGCTCCACGGTTTCATCGTACACATCTTGCGTCTTGTTTGGAGATTCCCACACGCCGTAGTTCTTGGTGACCATTACCACAGCATAGACAACGCCCGCTCGCAAAATCAGGCCCACAACCATTTTACCAAGCTTCGAAATCCCGAACTCCTTATGATGTTTCCTGTGTGACCTCGAATTTTCGACAGCCAATGCCAGGGTATTTAGGATGGCACCTTTTTGCCACACGTTAAGTCGTCCTTAATCTTGGGTGATCGCAGTGCTCGCTTTGATTTCGTTTGATTGGAAACTTGCATTTCTCCGGGAATGTTTTCAAAGAATCTTGCATTACTGCTCTCCGAAAAGATTTCGCCATAACGTTCAGAACGATAATCTCCATAGAATTTCGATATCTCCGAGTCGTTGCTGCTTTTGAAACTTCTCCTTGGGCAGTCAAAACCATGGGATAGTATCTTCTGGGTCTGGTGCTTAAAGTCCTTAGCCCAGTCGGGCATCCGAAGGGAGAAGTAGAATCCCCGCTGGAGAGCCACACTGCAGTTGTTTGCAGCCACGAAACTGAACTGGCTTTCTCCAAGTGGGAAAGGACCCATTGATAGATTCTCAGAGGCTGCAATGACCACCTGCTCTTCCG

 

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