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CR01633

CRIMIC CR01633

Gene RpL10

CG17521

Information

Donor Location Cytology FBti Stock number
pM37-p0:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
TCGGTTTGCGACGAAACCTTTTGTGC ATACGAGCCCACACACGTTCCACTAT

Images

Construct Design Sequences

sgRNA sequence TTTGGGTAACACGTCGAAATCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGAGCGCCCGTTTTGAAATG CGGCGTTAACTAAGGCAAAGA CGACGTGTTACCCAAAATCTTGT TTTCATCCTCCAGCTACCGC
 
Amplicon 5' Amplicon 3'
AGAGCGCCCGTTTTGAAATGTAGATCACGCTCGTATATTTTGAAACTGTCTTTGGTTAATTGGTTTCCAAAGAAATTAGAGACTCAAAAAATAAATTCCAAATTACTGGGTAATAAAATGTTTCCGAATCGACAGAGAATACCTACAATCGTGTTGTTGCTTGAGATTGTAAAAAGATGTATAAAATTGTATATAATTATGTATATAGAAACGCAATAAAAACGAGAAAAGGAGTACCCATTCTAGTCGGACCCGTATTACCTTCAATTTGCTTTTAATCTAATTAACCTTTTCTACACGCTTAAAACAATCTTTCTTTTTTTCTTTCTACGAAAGCAAATCTGCTGCTGCGAAATAATAAAGTTAAAACAATACAAAGGGCATTCTGAGCTTCCTACCAAAAAGCAACGCATATATATATGTGCTCAGGAAATGGTAATTTTGTATATTGTTTCTGAGTTACGAAACTTTAAGTGACATTTTATTTCAATGTGTTATTAAGCATAAACATCACGCTGAGCTTTTTCCCATGCGGCAATCGGGCCATGCTCTGGGCGGTATTTCACATTGCAACCATCAGGCTCAAGGCGGTTGTCATCGCGAAGTTCTTCATAACGCTCACGTTCGTATTTGGTGAATCCCCACTTCTTTGAAACATAGATCTAAAAATATATTTAAGAAAAACATTGTTTTACAAAATCTGTTTTCTTAGTTTTTCGTAATTTCTGTAAACCATCTTAAGTTCGTGCGCACCGAAGCTTAAAAATTTAAACAAATGTACCTAATGGACCAAAATACGATTTTCCAAAACCGTCTTCAAAATTCTTTACCTGCACGAACCATAGTCGTAGTCTTACCAAATGACCGCTAACGGACTAAAGAATAAACATCCTTTAATGCTTTTTTTATACCTACTAGGAGAGTTTAGCGGAAACGGAAATAATTAAGTTAAGAAAAATAGTAGGAAGTATCGAGTGGTTATCAGCTTGCAACAAAGACCTAAGCGTATGCTCGACAAGCGCGGAAGATTCCACATTTCTCCTTTTTATCCCTAGCGAAATGCATTAAATTTTTGTCGAGGGTCTTGCGGGTTACACTAGCAATCATAAAATCTTTGCCTTAGTTAACGCCG CGACGTGTTACCCAAAATCTTGTCACCAATTTGAACGAAAATAAGAAGTTTAAGATTCAACACTTTTTTCTTTTAAATGAGGTATATAGTTATAAATAATTTATAATACTATTTTAAAGTTCTTTAGATATTGATTTAGGTTCGCTAGTAATATTATTTTTAGCTCATTCCCAACAAATTAACTGTGGCGATATTAGAATAGCCTCGCCCATGACATGAAATAAAAGCGATTACTAATTTTCCATGTGGTTTGTTCAGACTTCGATGTTTGAAAAAATGGGTTAATATACTATTGGGTTAAATACTATTGTAAGTGACTCGCTATACTTGCCTTTTGACGTCCAGGGAACTTAAACTTAGCACGCCGCAAAGCTTCAATAACTTGAGCCTTGTAACGATCGCTAGAGCGGACAGACATAATGGGTTGACCAATACGAACTCGAGCAACCGTGCCCTGCGGTTTTCCAAACGCTCCTCGCATTCCAGTTTGAAGCCTAGTGCAAAAGAGGTCATAAGAATACAGTAGTTAACACAGATATGAAATATTATTTACCTATCAGCTCCAGCGCACGACAACATTTTGTTGATGCGAATGACGTGGAATGGATGCAGGCGCATTCTGATGTGGAACTGGTCCTTACCGCAGTACTTCACCAAGTACTTGTTGCAACAAATGCGTCCAGCTTCCAAAGCTTCACTACTCAGCTGTTCGTATTCATCAGAAACCAAATGAACACACAGGGGAAAATCCTCAACTGTAGCTTTTTTTCTTCCCAAATCAAATATACGAATTTTTGGATCAGGGACACCACGGCAAAACCGAGATTTAGGGTACGGCTTATTTTTGCAATAGCGGTAGCTGGAGGATGAAA
 
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