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CRIMIC CR70013

Gene AstC

CG14919

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2L:11076165..11081399	32D2	FBti0220377

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGTTTAAGCTGCAGCTCCATATCCG	GCACACACATTGAGGAATGCAG

Images

Construct Design Sequences

sgRNA sequence	TAACTATTGACTATTAAACAAGG
sgRNA2 sequence	GGAAGTAAGTTCCCGGACAGTGG

 

left_200bp	right_200bp
TCCACTGAATCCCGGGACCTTTAATTAAAAAATGTGGCTAGCTGAACTTCATGGCGAAGTTTTTTGCGTGAACTCGCCTCTTGACAACCTTTGTGCATCGGGATTAGCGGGGCACAAAGTCCTGGAGGTTTTTCTTTGAAGTTTTGCAAGCATTTGCATTAATTGGAATGCTACAACAGTTCATAACTATTGACTATTAA	CAGTGGGAAGAATATCCGGAACCCAGAGCTCTTGGTTAAACTAACAATTCAAGCATTTCAACAAACCAAAACCAAAAAAATAAGCCACAACACAAAGATTAGCATAATGGATAAACGCAAATGTTTAGAGCAATTAAACTAAAGCTAAGACTCAACTATATCCATATACATATATATACAACTTATCCTTTGAATGTCTA

 

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