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CRIMIC CR70044

Gene iPLA2-VIA

CG6718

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3L:9860217..9864349	67C11	FBti0216210	92695

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCATTGGCCAAAGATGTGTAC	GGATGTGGTATTGGAATCCGAGG

Images

Construct Design Sequences

sgRNA sequence	TTCTCAACTAGTCTACTAAACGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCATCCACATTAGCCAGCTCATCCGATCAGATTGGCCCAGTTCAAAGTACACATTCAGCATCCGCAGCAGCAAATTCCGCACCCTTGAGTCATGGCGTGGATGGCGTTAGGGGCACTAGCCTCCGGTTTCGGTATTCACCCCCCTTTCAATCATTACTGCTCTTCCTCCTCCTGTTCATGTTCATGTCGTGTCACCGTTT	AGTAGACTAGTTGAGAATGTATTAATGTGTCTTATGACCAACGCATTCGCCTTATCTAATGACATTGCCTTTCAATTTTGTACGACTACAGTTCTACAGCGCCTGCTGGGTGTCGATCAGCCGGCCAATAAGGTCGTGGAAATTAAGAGCGAAGCGCTGGGGACACTTCAAGTCCTGGCCCGCGATGACGCCATGCTCCT

 

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