CR70059

CRIMIC CR70059

Gene TBPH

Donor	Location	Cytology	FBti	Stock number
int100_PM37_kG4	2R:23859067..23862593	60A4-60A5	FBti0220975	Order from Bellen Lab

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGATTCCACACTCACAAACG	CTCCTCTAAAATGAGGCTGCGC

sgRNA sequence	TCTAGCAGCCTTCTAGGAAATGG
sgRNA2 sequence	TAACCACATCATTGGGTGACAGG

left_100bp	right_100bp
GTTGACTGAATGACATGGTGGATCATTTCTAGAGCGAAGTGTGAGCCCCTGCGGCGATAGAGTTCTTTAATCCCCTCCTCGTTTCTAGCAGCCTTCTAGG	ACCCAATGATGTGGTTAATGTGGTTAACCAACACCTCTGTAGCTTACTTAAATTTACATATATGTATTCCACATACAAACCAGTTAAGAATTGTATTTAA