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CRIMIC CR70080

Gene PIG-A

CG6401

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:17737622..17739685	54E4-54E5	FBti0220391

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	CGATGATCTGCTACCTGTTTGTGC	ATGGAGCGACAAGGAGGCTAGATT

Images

Construct Design Sequences

sgRNA sequence	TATGTGGTATGTCAAATTACTGG
sgRNA2 sequence	TTGGCTAGTTGATGGAAGAATGG

 

left_200bp	right_200bp
CACTTTTATACATATTCGTGGCACATTAATTTAATGTTATATTCAAATCGAAATTGGGCCCCAGTACCGATTAGCATTTTCTTCACCTTTCAACACTACTACATCGACCACTCTTTGGCCATTGTGCACACTGTTGTTTTTTGCTTGCCGATTTTTGTTTAACATATTGTATTTATTTGTTTATATGTGGTATGTCAAAT	TTCCATCAACTAGCCAATTATATATTTATGCGATAGATTTTATAACCCTTGTTGTGTTTATTTGTTGTCCATAGTAGTAGTCGAAGTGATAGTTCCCTTAAACAATATGATAATCCATTCTTATGATAAAATTAACCCAAACGGCGCTGCTGATCTACGCCGATAAAATATTCCGCCCACAGCAACGTTTTTATAAAGAA

 

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