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CRIMIC CR70107

Gene dmrt99B

CG15504

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:29692591..29697025	99B8	FBti0216221	92707

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTGAAGGGACACAAACGCTACTG	TTCACTCACCAGGACTCTCATCCA

Images

Construct Design Sequences

sgRNA sequence	TTGATCAGTGGCGTGTAACAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGAAAGAATTGGAATAAATGTTATAGAATGAAGAAGATTGCTGGATTGATATATATAGTTCAGTTAAAGAGAACCTATGTGTGCTTAAAAGCAGGGTTTTTTTTATAGTATAGCATGGAAATCAAGTACTACTTCAAAATTGTATAACTCCTCTGTTGATTTCAGTTAAAAATCATAGTAATATTAATGCATCTCCCTGT	TACACGCCACTGATCAAAGATCCGTTTTCCGCCCATGCCCTCCTTGTTGCAGGTTGCCCTACGACGCCAACAGGCCCAAGAAGAGAACGAGGCCCGCGAGCTGGGTCTGCTCTACACTTCGGTTCCTGGCCAGCAGAATGGCAGCGACAGTGCTACGCCCACTCCGCACAGCCCCAACCACAGCGGCAGCGGTGGCTCGG

 

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