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CRIMIC CR70137

Gene Su(z)12

CG8013

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:19918242..19923462	76D4	FBti0216231	92717

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGGCCTTTGAGAGTAAGTAGAAC	CGTGTGATCTTGTACAGCGTTGTC

Images

Construct Design Sequences

sgRNA sequence	AATATCCCAGACATTCTAAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCACGCAGATCTATCGCTACCTGCGCAATCGACACGAGACAAACGTAAGTTGATATCCCTTCCAAGTATTTGGGTCCCATACATCATTGCAGACTAAGTTTCAAATAAGAGATTCCTCGAATTCAGGTTACAACGCAATCGCAATTAATACTCAGCTTCAACTTTGCTGCTCCAGTTATGGAGTTATCTTAAATCCCTTT	AGAATGTCTGGGATATTTAGCTCCACAAGTTCTGCACCACTCATAACTATACATACCCCTTCTCAAATACAGCCCATCTTCCTCAACCGCACGCTCAGCTACATGAAGGAACGGATGTCGCGGAACAACAAGAAGCGGATCAGCTTCCAGGTAAACTCCATGCTGGAGAGCCTAACACAAAAGTCGGAGGCGGTGAGCCA

 

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