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CRIMIC CR70283

Gene Qsox2

CG17843

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:21913747..21915546	93F14	FBti0220451	95020

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGAGATCCTATGGTTGTGGGAA	ATAAGTCAGTCCGCTTGTCAGGTG

Images

Construct Design Sequences

sgRNA sequence	ATTCAATAGCAAGGGCTCTTTGG
sgRNA2 sequence	ACAAAACTCGTTCATGCAATAGG

 

left_200bp	right_200bp
CAATTTTTACTTGCATTTTTTCCGTAATATACACACACTTAAAATATATATGAAATTCATGAATTCTTTTGGGTGTGCCTGGATAGAATAGCGAGTCACCAAATTAAGAAAACCTGTTTTGAGAGAGAGATTTGGGGTATGGTGGGGGAGACTTAACAACCGGAGCAGGGCGAATCCAAATGTGGCTATAATTAGCAAAGAGCCCTTGCTATTGAATCAGTTGAAGTGGCTCATTTGACTTGTGAGCACAGTGTGGAA	GCATGAACGAGTTTTGTGAACCAATTTTATTAAATAAATTAAATATAAATAACATAGACAAGATTTTACTCGAAACGATTCAAGTCGAAAAGCTCTTATGTCCGCTATCCTTTAAGGATTGCGTATACAGAAATGCGTGTTTGACCCTCGATAGTGACATGAAAATTGCATAAATTTGCTAAATTAAGCGTTTAGATTAA

 

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