MiMIC RMCE Technology

BDSC No.	Genotype	Insert Chr	Phase
63287	y1 w*; P{w+mW.hs=FRT(PT-GFSTF.0)}2	2	0
64198	y1 w*; P{w+mW.hs=FRT(PT-GFSTF.1)}2	2	1
63288	y1 w*; P{w+mW.hs=FRT(PT-GFSTF.2)}2	2	2
63289	y1 w*; P{w+mW.hs=FRT(PT-GFSTF.0)}3	3	0
63290	y1 w*; P{w+mW.hs=FRT(PT-GFSTF.1)}3	3	1
63291	y1 w*; P{w+mW.hs=FRT(PT-GFSTF.2)}3/TM6C, Sb1 Tb+	3	2
You will also need: 33216 P{ry[+t7.2]=hsFLP}12, y[1] w[*] M{vas-int.B}ZH-2A; Pri[1]/TM3, Sb[1]

X Chr Insertions - These have not been tested.
BDSC No.	Genotype	Insert Chr	Phase
64264	P{w[+mW.hs]=FRT(PT-GFSTF.0)}1, y[1] w[*]	X	0
64265	P{w[+mW.hs]=FRT(PT-GFSTF.1)}1, y[1] w[*]	X	1
64266	P{w[+mW.hs]=FRT(PT-GFSTF.2)}1, y[1] w[*]	X	2

RMCE Crossing Scheme for a Typical Chr 3 MiMIC insertion

Download: PCR verification protocol (updated 12/13/17)