Tool Stocks
The stocks required for the crossing scheme are currently available from the Bellen lab but are on their way to the Bloomington Drosophila Stock Center for distribution. Please use our order page to request them and use the line number below to refer to which strain you need for your experiment:
| Line No. | Genotype | Insert Chr | Phase |
|---|
| 1 | P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; P{w+mW.hs=FRT(PT-GFSTF.0)}2; Pri1/TM6B, Tb1 | 2 | 0 |
| 2 | P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; P{w+mW.hs=FRT(PT-GFSTF.1)}2; Pri1/TM6B, Tb1 | 2 | 1 |
| 3 | P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; P{w+mW.hs=FRT(PT-GFSTF.2)}2; Pri1/TM6B, Tb1 | 2 | 2 |
| 4 | P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; Sp/CyO; P{w+mW.hs=FRT(PT-GFSTF.0)}3 | 3 | 0 |
| 5 | P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; Sp/CyO; P{w+mW.hs=FRT(PT-GFSTF.1)}3 | 3 | 1 |
| 6 | P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; Sp/CyO; P{w+mW.hs=FRT(PT-GFSTF.2)}3 | 3 | 2 |
| * Note: Due to the leakiness of the hsFLP, these stocks need to be kept at 18°C. |
| Insertions Only |
| BDSC No. | Genotype | Insert Chr | Phase |
|---|
| 63287 | y1 w*; P{w+mW.hs=FRT(PT-GFSTF.0)}2 | 2 | 0 |
| 64198 | y1 w*; P{w+mW.hs=FRT(PT-GFSTF.1)}2 | 2 | 1 |
| 63288 | y1 w*; P{w+mW.hs=FRT(PT-GFSTF.2)}2 | 2 | 2 |
| 63289 | y1 w*; P{w+mW.hs=FRT(PT-GFSTF.0)}3 | 3 | 0 |
| 63290 | y1 w*; P{w+mW.hs=FRT(PT-GFSTF.1)}3 | 3 | 1 |
| 63291 | y1 w*; P{w+mW.hs=FRT(PT-GFSTF.2)}3/TM6C, Sb1 Tb+ | 3 | 2 |
| You will also need: 33216 P{ry+t7.2=hsFLP}12, y1 w* M{vas-int.B}ZH-2A; S1/CyO; Pri1/TM6B, Tb1 |
| X Chr Insertions - These have not been tested. |
| BDSC No. | Genotype | Insert Chr | Phase |
|---|
| 64264 | P{w[+mW.hs]=FRT(PT-GFSTF.0)}1, y[1] w[*] | X | 0 |
| 64265 | P{w[+mW.hs]=FRT(PT-GFSTF.1)}1, y[1] w[*] | X | 1 |
| 64266 | P{w[+mW.hs]=FRT(PT-GFSTF.2)}1, y[1] w[*] | X | 2 |
|
