The Gene Disruption Project (GDP) is using a non-targeted transposon mutagenesis strategy to create a public collection of insertion mutants. The collection can be searched online and new mutants may be requested while they are being balanced. Balanced stocks are available from the Bloomington Drosophila Stock Center. We have currently contributed over 18,000 insertions to the public collection. |
Current Phase |
In the current phase of our project we are creating CRIMIC lines. For more information about this technique see the CRIMIC section. This project is a collaboration among the laboratories of Hugo Bellen (Baylor College of Medicine), Norbert Perrimon (Harvard Medical School) and Allan Spradling (Carnegie Institution for Science) and is supported by the National Institutes of Health (R01GM067858) and the Howard Hughes Medical Institute. The Perrimon lab designs and clones the CRIMIC constructs (Jonathan Zirin, Yanhui Hu, Stephanie Mohr, Rong Tao and Colby Devereaux); injection, balancing and quality control checks are performed in the Bellen Lab (Yuchun He, Ying Fang, Wen-Wen Lin, Qiaohong Gao, Liwen Ma, Junyan Fang, Zhihua Wang, Ming Ge and Jiangxing Lv) and stock data transfer to FlyBase and BDSC is handled by Bob Levis (Carnegie). |
Previous Phases |
Phase I |
This project was a collaboration among the laboratories of Hugo Bellen (Baylor College of Medicine), Roger Hoskins (Lawrence Berkeley National Laboratory) and Allan Spradling (Carnegie Institution of Washington), supported by the National Institutes of Health (R01GM067858) and the Howard Hughes Medical Institute. The flies were generated, selected, balanced and maintained by Yuchun He, Ying Fang, Zhihua Wang and Jianping Li in the Bellen lab. The inverse PCRs and sequencing were performed by Martha Evans-Holm in the Hoskins lab. Mapping information was obtained by Ben Booth in the Hoskins lab followed by manual curation by Bob Levis and Allan Spradling in the Spradling lab. A variety of transposons were used as we continued to upgrade to strive for better coverage, and we also sequenced and selected insertions from donated collections for deposition in the BDSC. |
Phase II |
During this part of the project, the MiMIC insertion was developed in the Bellen lab by Koen Venken. About ~17,500 MiMIC insertions were generated at Baylor College of Medicine by the Bellen Lab team and were end-sequenced by the Hoskins lab at Lawrence Berkeley National Laboratory. The sequence information was curated by Bob Levis at Carnegie Institution for Science. A subset of these insertions were selected for RMCE conversion using the GFSTF and TG4 tags using both the injection and crossing methods. |
The web database and strain requests are being handled by Guang Lin in the Bellen lab. |
Please reference our publications, which are also found on the Downloads page, as well as the Bloomington Drosophila Stock Center, when utilizing these stocks. References
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