Transposons Featured in this Database
These are schematic drawings highlighting the principal components and are not drawn to scale.
Lines beginning BG, KG, EY, MB, MI: Generated by the Gene Disruption Project. Please cite Bellen et al. (2004) Genetics 167:761-781 (BG, KG, EY); Bellen et al. (2011) Genetics 188:731-743 (MB); or Venken et al. (2011) Nature Methods 8:737-743 (MI).
- Reference for this P element: Lukacsovich et al. (2001) Genetics 157:727. [Abstract]
- Sequence
- Genetic background used during the screen: w1118 (stock)
- Transposase: Δ2-3
- Marker for selection: w+ eye color
|
![BG](images/BG.gif) |
- Reference for this P element: Roseman et al. (1995) Genetics 141:1061.
[Abstract]
- Sequence - FlyBase-compiled
- Genetic background used during the screen: y1; ry506 (stock)
- Transposase: Δ2-3
- Marker for selection: w+ eye color or y+ body color
|
![KG](images/KG.gif) |
- Reference for this P element: Bellen et al. (2004) Genetics 167:761-781.
[Abstract]
- Sequence
- Genetic background used during the screen: y1 w67c23 (stock)
- Transposase: Δ2-3
- Marker for selection: w+ eye color or y+ body color
|
![EY](images/EY.gif) |
- Mi{3xP3-EGFP.ET1} synonym
- Reference for this transposable element: Metaxakis et al. (2005) Genetics 171:571.
[Abstract]
- Sequence
- Genetic background used during the screen: w1118 (isogenized)
- Transposase: Minos
- Marker for selection: GFP
- Can plasmid rescue
|
![MB](images/MB.gif) |
- Reference for this transposable element: Venken et al. (2011) Nature Methods 8:737-743.
[PDF] [Suppl]
- Allows RMCE-mediated integration of any DNA fragment, such as fluorescent protein tags
|
![MI](images/MI.gif) |
Other Collections
All others presented in this database were generated by other teams and analyzed/selected as part of the Gene Disruption Project. Please cite the appropriate reference. All lines are available from the Bloomington Stock Center.
- Lethal and male/female sterile lines from Spradling et al. (1999) Genetics 153:135-177. [Abstract]
|
![PZ](images/PZ.gif)
|
- Göttingen X chromosome lethals (line names start with G0). Reference: Peter et al. (2002) EMBO Rep 3:34. [Abstract]
- Lethal and male/female sterile lines from Spradling et al. (1999) Genetics 153:135-177. [Abstract]
- SH lines. All on FRT chromosomes. Donated to Szeged Stock Centre by Steven Hou. Reference: Oh et al. (2003) Genetics 163:195. [Abstract]
- S lines. All on FRT chromosomes. Donated by Szeged Stock Centre. References: Deak et al. (1997) Genetics 147:1697-1722 [Abstract] (original insertion); Bellotto et al. (2002) Int. J.
> Dev. Biol. 46:149-157 [Abstract] (crossing onto FRT).
|
![PlacW](images/pLacW.gif)
|
- EP lines. Reference: Rørth (1996) PNAS 93:12418. [Abstract]
- Donated by GenExel (Korea), rebalanced and flanks confirmed by GDP. Line names start with G.
|
![EP](images/EP.gif)
|
- PA lines. Donated by Brian Ring and Dan Garza.
|
![PA](images/PA.gif)
|
- PC lines. Donated by Brian Ring and Dan Garza.
|
![PC](images/PC.gif)
|
- PL lines. Reference: Häcker et al. (2003) PNAS 100:7720-7725. [Abstract]
|
![PL](images/PL.gif)
|
- DG lines. Reference: Huet et al. (2002) PNAS 99:9948. [Abstract]
|
![DG](images/DG.gif)
|
- LA lines. Donated by John Merriam.
- DP lines. Donated by DeveloGen.
- GG lines. Reference: Beinert et al. (2004) Chromosoma 113:271-275. [Abstract]
|
![Mae-UAS](images/LA.gif)
|
- HP lines. Donated by DeveloGen.
|
![EPg](images/EPg.gif)
|
- Exelixis collection - line names start with "c"
- Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]
|
![PB](images/PB.png)
|
- Exelixis collection - line names start with "e"
- Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]
|
![RB](images/RB.gif)
|
- Exelixis collection - line names start with "f"
- Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]
|
![WH](images/WH.gif)
|
- Exelixis collection - line names start with "d"
- Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]
|
![XP](images/XP.gif)
|
- GT lines
- Donated by University of Cambridge, UK.
|
![GT](images/BG.gif)
|