Transposons Featured in this Database

These are schematic drawings highlighting the principal components and are not drawn to scale.

Lines beginning BG, KG, EY, MB, MI: Generated by the Gene Disruption Project. Please cite Bellen et al. (2004) Genetics 167:761-781 (BG, KG, EY); Bellen et al. (2011) Genetics 188:731-743 (MB); or Venken et al. (2011) Nature Methods 8:737-743 (MI).

BG Lines - P{GT1}

  • Reference for this P element: Lukacsovich et al. (2001) Genetics 157:727. [Abstract]
  • Sequence
  • Genetic background used during the screen: w1118 (stock)
  • Transposase: Δ2-3
  • Marker for selection: w+ eye color
 BG

KG Lines - P{SUPor-P}

  • Reference for this P element: Roseman et al. (1995) Genetics 141:1061. [Abstract]
  • Sequence - FlyBase-compiled
  • Genetic background used during the screen: y1; ry506 (stock)
  • Transposase: Δ2-3
  • Marker for selection: w+ eye color or y+ body color
 KG

EY lines - P{EPgy2}

  • Reference for this P element: Bellen et al. (2004) Genetics 167:761-781. [Abstract]
  • Sequence
  • Genetic background used during the screen: y1 w67c23 (stock)
  • Transposase: Δ2-3
  • Marker for selection: w+ eye color or y+ body color
 EY

MB lines - Mi{ET1}

  • Mi{3xP3-EGFP.ET1} synonym
  • Reference for this transposable element: Metaxakis et al. (2005) Genetics 171:571. [Abstract]
  • Sequence
  • Genetic background used during the screen: w1118 (isogenized)
  • Transposase: Minos
  • Marker for selection: GFP
  • Can plasmid rescue
 MB

MI lines - Mi{MIC}

  • Reference for this transposable element: Venken et al. (2011) Nature Methods 8:737-743.
    [PDF] [Suppl]
  • Allows RMCE-mediated integration of any DNA fragment, such as fluorescent protein tags
 MI

Other Collections

All others presented in this database were generated by other teams and analyzed/selected as part of the Gene Disruption Project. Please cite the appropriate reference. All lines are available from the Bloomington Stock Center.

P{PZ}

  • Lethal and male/female sterile lines from Spradling et al. (1999) Genetics 153:135-177. [Abstract]

PZ

P{lacW}

  • Göttingen X chromosome lethals (line names start with G0). Reference: Peter et al. (2002) EMBO Rep 3:34. [Abstract]
  • Lethal and male/female sterile lines from Spradling et al. (1999) Genetics 153:135-177. [Abstract]
  • SH lines. All on FRT chromosomes. Donated to Szeged Stock Centre by Steven Hou. Reference: Oh et al. (2003) Genetics 163:195. [Abstract]
  • S lines. All on FRT chromosomes. Donated by Szeged Stock Centre. References: Deak et al. (1997) Genetics 147:1697-1722 [Abstract] (original insertion); Bellotto et al. (2002) Int. J. > Dev. Biol. 46:149-157 [Abstract] (crossing onto FRT).

PlacW

P{EP}

  • EP lines. Reference: Rørth (1996) PNAS 93:12418. [Abstract]
  • Donated by GenExel (Korea), rebalanced and flanks confirmed by GDP. Line names start with G.

EP

PBac{5HPw[+]}

  • PA lines. Donated by Brian Ring and Dan Garza.

PA

PBac{3HPy[+]}

  • PC lines. Donated by Brian Ring and Dan Garza.

PC

PBac{GAL4D,EYFP}

  • PL lines. Reference: Häcker et al. (2003) PNAS 100:7720-7725. [Abstract]

PL

P{wHy}

  • DG lines. Reference: Huet et al. (2002) PNAS 99:9948. [Abstract]

 

DG

P{Mae-UAS.6.11}

  • LA lines. Donated by John Merriam.
  • DP lines. Donated by DeveloGen.
  • GG lines. Reference: Beinert et al. (2004) Chromosoma 113:271-275. [Abstract]

Mae-UAS

P{EPg}

  • HP lines. Donated by DeveloGen.

EPg

PBac{PB}

  • Exelixis collection - line names start with "c"
  • Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]

PB

PBac{RB}

  • Exelixis collection - line names start with "e"
  • Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]

RB

PBac{WH}

  • Exelixis collection - line names start with "f"
  • Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]

WH

P{XP}

  • Exelixis collection - line names start with "d"
  • Reference Thibault et al. (2004) Nature Genetics 36:283-287. [Abstract]

XP

P{GT1}

  • GT lines
  • Donated by University of Cambridge, UK.

GT