MiMIC Cloning Vectors

The MiMIC constructs are available for order from the Drosophila Genomics Resource Center. DGRC barcode ordering numbers are shown below. See Venken et al. (2011) Nature Methods 8:737-743 [PDF] [Suppl] for more information about the technique.

pMiLR-attP1-2-yellow-SA-EGFP

1321

MiMIC or Mi{MIC} vector

pBS-KS-attB1-2

1322

General cloning and correction plasmid (Sequence)

Gene Trap
pBS-KS-attB1-2-GT-SA	1323	General gene trap plasmid (Sequence)
pBS-KS-attB1-2-GT-SA-mCherry-SV40	1324	mCherry gene trap plasmid
pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA	1325	GAL4 gene trap plasmid
pBS-KS-attB1-2-GT-SA-Flpo-SV40	1326	Flp gene trap plasmid
pBS-KS-attB1-2-GT-SA-QF-Hsp70	1296	QF gene trap plasmid

Phase 0
pBS-KS-attB1-2-PT-SA-SD-0	1297	General splice phase 0 protein trap plasmid (Sequence)
pBS-KS-attB1-2-PT-SA-SD-0-EGFP-FlAsH-StrepII-TEV-3xFlag	1298	Splice phase 0 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid
pBS-KS-attB1-2-PT-SA-SD-0-mCherry	1299	Splice phase 0 mCherry plasmid
pBS-KS-attB1-2-PT-SA-SD-0-EBFP2-3xMyc	1300	Splice phase 0 EBFP2-3xMyc plasmid
pBS-KS-attB1-2-PT-SA-SD-0-TagRFP-T-3xHA	1301	Splice phase 0 TagRFP-3xHA plasmid
pBS-KS-attB1-2-PT-SA-SD-0-HRP-S	1302	Splice phase 0 HRP-S plasmid
pBS-KS-attB1-2-PT-SA-SD-0-Dendra-V5	1303	Splice phase 0 Dendra-V5 plasmid
pBS-KS-attB1-2-PT-SA-SD-0-KillerRed-V5	1304	Splice phase 0 KillerRed-V5 plasmid

Phase 1
pBS-KS-attB1-2-PT-SA-SD-1	1305	General splice phase 1 protein trap plasmid (Sequence)
pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag	1306	Splice phase 1 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid
pBS-KS-attB1-2-PT-SA-SD-1-mCherry	1307	Splice phase 1 mCherry plasmid
pBS-KS-attB1-2-PT-SA-SD-1-EBFP2-3xMyc	1308	Splice phase 1 EBFP2-3xMyc plasmid
pBS-KS-attB1-2-PT-SA-SD-1-TagRFP-T-3xHA	1309	Splice phase 1 TagRFP-3xHA plasmid
pBS-KS-attB1-2-PT-SA-SD-1-HRP-S	1310	Splice phase 1 HRP-S plasmid
pBS-KS-attB1-2-PT-SA-SD-1-Dendra-V5	1311	Splice phase 1 Dendra-V5 plasmid
pBS-KS-attB1-2-PT-SA-SD-1-KillerRed-V5	1312	Splice phase 1 KillerRed-V5 plasmid

Phase 2
pBS-KS-attB1-2-PT-SA-SD-2	1313	General splice phase 2 protein trap plasmid (Sequence)
pBS-KS-attB1-2-PT-SA-SD-2-EGFP-FlAsH-StrepII-TEV-3xFlag	1314	Splice phase 2 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid
pBS-KS-attB1-2-PT-SA-SD-2-mCherry	1315	Splice phase 2 mCherry plasmid
pBS-KS-attB1-2-PT-SA-SD-2-EBFP2-3xMyc	1316	Splice phase 2 EBFP2-3xMyc plasmid
pBS-KS-attB1-2-PT-SA-SD-2-TagRFP-T-3xHA	1317	Splice phase 2 TagRFP-3xHA plasmid
pBS-KS-attB1-2-PT-SA-SD-2-HRP-S	1318	Splice phase 2 HRP-S plasmid
pBS-KS-attB1-2-PT-SA-SD-2-Dendra-V5	1319	Splice phase 2 Dendra-V5 plasmid
pBS-KS-attB1-2-PT-SA-SD-2-KillerRed-V5	1320	Splice phase 2 KillerRed-V5 plasmid