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CRIMIC CR00002

Gene CG13887

CG13887

Information

Donor	Location	Cytology	FBti	Stock number
pM14	3L:839049..839051	61D2	FBti0200709	81130

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GGCCATCAGACCCTTCAGCAGATC	TCTGGTAGGTGCGCTGGTGGACA

Images

Construct Design Sequences

sgRNA sequence	GTGGTGGCTTCTGTTTATCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATTTCTGCTTACAGGCGGC	TCGTGGCCCAACATATGTGT	AAACAGAAGCCACCACTCGA	CAAACAGGTTGCGACGTCTG

 

Amplicon 5'

Amplicon 3'

CATTTCTGCTTACAGGCGGCAGTCGAAAAAAGCGAAAATCCAAGAAGCGAAAATCGAACGAAAAAGGGAATTAATAAAACATTAAAAATGAGTTTGGTGTGGACTTTGATCGCCGGATTTCTCTATGCGGAAATCGCACTGGTCCTTCTGCTGGTGCTGCCGGTGCTCACTCCTTACCGCTGGAACCGGTTCTTCAAGTCCAAATTCCTATCGATGTTGGGACAACAGGCGCACATATATTTCCTGCTGATCATGGGAATTCTGGTCATTTTCCTCCTGGAGGCCATTCGCGAGATGCGCAAGTACTCCGGCTTGCAGCAGTCTAACGAGGTCCACTTGAACGTGGAGATGCAGCACAGCATGAAGCTGTTCCGTGCCCAGCGGAACTTCTACATCTCTGGATTCGCCATTTTCCTTGCCCTGGTCATCCGGCGACTGGTAAACTTGATCTGCACCCAGGCCAATCTCATGGCCCAGAGCGAGGCTTCCTTCAAGCAGGCACAGAGCGCCACTGCAGCCGCACGTTCCTTGCTGGAGAACAAGAACACCGAGAAGGCCAAGGAAGCCGGCGAGGATACCACTCTCATCGAGGTGAGTCCACGTCCCAAACCCCTGCATCCGATCCCCAGGAATGGCATGTCTAGAGAGCTAACACCTAAGACTTTGGAGTTCAATTGGCTACTACCAGCTGTAAAGATATGGCAATGCATATAGAAGGTGTGGTCAAAATATGGCGAGATAATGTCTTTAGCCGACTAAATCTATAAAATCTATACTTCATTCCACTTTCTGGTACATTTTCTCAATTGGTTTTGAATATAAGATACGAAAAGGCTTTAAATGTAGTCTATTAACGATATAGAACTTTAAATACCAGTATATATCATGATTAAAATCTTAAACGTAAACTTAAATCAGTTTGAAGACCGAGTGAATTTGTCATAGGCTTAGTGCTTTTGTTTTGACCGCAGCTAAACGTTTTGAAACCACACCCAAACACATATGTTGGGCCACGA

AAACAGAAGCCACCACTCGAAAGTTTAAACCTCTTGTTAACTCTTTAAACAGCTGTCAAGACTGCGGAGACGTGTGCAAGGACTCACCGCCCGAGTGTCCTAAGTATCCCCACCTAGAGCACCCACCTTATATCCATTCCCAAACACCCAAAACGAACAAAAATCCAATCGAAACTCAAACGAAATCCGAATTTTGCAGCTCAACAAGCTGCGCGAGCGCGTCCAGGAGCTAACCTCCGACTTGAATCGCGAAAAGAAGGACAAGGAGGCGGTTAAGTCGCAGGCGGAGAGCATCAACCGCGAGTACGACCGACTCACCGAGGAGTACAGCAAGCTGCAGAAGAAGATAACCATCGGCGGAGGCGGAAACAAGGATGATTAAACTCACTGGAGCCAAGTCCAACATTTAAATAACGATAAGACTTATAAGCATGGCGGTGGGGCAGCGAAAGCAGGCACATTTAAAAGATTATAACGATTTCAAATCGATAGAAAGCACACACATCTTATTGTTAATCCTCATAGATACAACTACATATACCATTTGATAATGATAATGGGGTATATTGATAATGGTCCCTCAACTCAATGTTCGTTTCGTCCGAAGTTTTGTATTTAATTCGTATGTGTTAAGTTTTGTTTGCCACAGTAACACACCCAAAAAGCGTCGTGGCTATTAAAAGAACAACCAAAAAGTACCCATCCAGTTGTGATCGATTCGATGATTCATCTGTTGGCGCGGATAGTCCACATCATGTCTGATAGTACACGGCAATGACGTCAGACGTCGCAACCTGTTTG

 

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