CR00002

CRIMIC CR00002

Gene CG13887

CG13887

Information

Donor Location Cytology FBti Stock number
pM143L:839049..83905161D2FBti020070981130

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK GGCCATCAGACCCTTCAGCAGATC TCTGGTAGGTGCGCTGGTGGACA

Images

Construct Design Sequences

sgRNA sequence GTGGTGGCTTCTGTTTATCGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CATTTCTGCTTACAGGCGGC TCGTGGCCCAACATATGTGT AAACAGAAGCCACCACTCGA CAAACAGGTTGCGACGTCTG
 
Amplicon 5' Amplicon 3'
CATTTCTGCTTACAGGCGGCAGTCGAAAAAAGCGAAAATCCAAGAAGCGAAAATCGAACGAAAAAGGGAATTAATAAAACATTAAAAATGAGTTTGGTGTGGACTTTGATCGCCGGATTTCTCTATGCGGAAATCGCACTGGTCCTTCTGCTGGTGCTGCCGGTGCTCACTCCTTACCGCTGGAACCGGTTCTTCAAGTCCAAATTCCTATCGATGTTGGGACAACAGGCGCACATATATTTCCTGCTGATCATGGGAATTCTGGTCATTTTCCTCCTGGAGGCCATTCGCGAGATGCGCAAGTACTCCGGCTTGCAGCAGTCTAACGAGGTCCACTTGAACGTGGAGATGCAGCACAGCATGAAGCTGTTCCGTGCCCAGCGGAACTTCTACATCTCTGGATTCGCCATTTTCCTTGCCCTGGTCATCCGGCGACTGGTAAACTTGATCTGCACCCAGGCCAATCTCATGGCCCAGAGCGAGGCTTCCTTCAAGCAGGCACAGAGCGCCACTGCAGCCGCACGTTCCTTGCTGGAGAACAAGAACACCGAGAAGGCCAAGGAAGCCGGCGAGGATACCACTCTCATCGAGGTGAGTCCACGTCCCAAACCCCTGCATCCGATCCCCAGGAATGGCATGTCTAGAGAGCTAACACCTAAGACTTTGGAGTTCAATTGGCTACTACCAGCTGTAAAGATATGGCAATGCATATAGAAGGTGTGGTCAAAATATGGCGAGATAATGTCTTTAGCCGACTAAATCTATAAAATCTATACTTCATTCCACTTTCTGGTACATTTTCTCAATTGGTTTTGAATATAAGATACGAAAAGGCTTTAAATGTAGTCTATTAACGATATAGAACTTTAAATACCAGTATATATCATGATTAAAATCTTAAACGTAAACTTAAATCAGTTTGAAGACCGAGTGAATTTGTCATAGGCTTAGTGCTTTTGTTTTGACCGCAGCTAAACGTTTTGAAACCACACCCAAACACATATGTTGGGCCACGA AAACAGAAGCCACCACTCGAAAGTTTAAACCTCTTGTTAACTCTTTAAACAGCTGTCAAGACTGCGGAGACGTGTGCAAGGACTCACCGCCCGAGTGTCCTAAGTATCCCCACCTAGAGCACCCACCTTATATCCATTCCCAAACACCCAAAACGAACAAAAATCCAATCGAAACTCAAACGAAATCCGAATTTTGCAGCTCAACAAGCTGCGCGAGCGCGTCCAGGAGCTAACCTCCGACTTGAATCGCGAAAAGAAGGACAAGGAGGCGGTTAAGTCGCAGGCGGAGAGCATCAACCGCGAGTACGACCGACTCACCGAGGAGTACAGCAAGCTGCAGAAGAAGATAACCATCGGCGGAGGCGGAAACAAGGATGATTAAACTCACTGGAGCCAAGTCCAACATTTAAATAACGATAAGACTTATAAGCATGGCGGTGGGGCAGCGAAAGCAGGCACATTTAAAAGATTATAACGATTTCAAATCGATAGAAAGCACACACATCTTATTGTTAATCCTCATAGATACAACTACATATACCATTTGATAATGATAATGGGGTATATTGATAATGGTCCCTCAACTCAATGTTCGTTTCGTCCGAAGTTTTGTATTTAATTCGTATGTGTTAAGTTTTGTTTGCCACAGTAACACACCCAAAAAGCGTCGTGGCTATTAAAAGAACAACCAAAAAGTACCCATCCAGTTGTGATCGATTCGATGATTCATCTGTTGGCGCGGATAGTCCACATCATGTCTGATAGTACACGGCAATGACGTCAGACGTCGCAACCTGTTTG
 

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