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CRIMIC CR00012

Gene DAT

CG8380

Information

Donor	Location	Cytology	FBti	Stock number
pM14	2R:16562128..16562183	53C7	FBti0199458	78865

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AAACCGTCTAAACTGAAGTCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCACCACGGCATATCCAAT	ACAGAAGGAAGCACCGTAGC	CATCCATGACGCTCGTCTCT	CCACCTCCCCATTTTCCCTC

 

Amplicon 5'

Amplicon 3'

AGCACCACGGCATATCCAATTCCTGTAAGTTAAAGTGTCTTATCCATGAAGTTCTTGTGATTAATTAGTTAGTAGTTAGTAATTACCTTTGAAGAGGGGCACCAAGCGACCCCAGCAGGTTATGGCACCCTTACGATTGTGCTGACCCAGGGCCAATTCCATATAGAACAGAGGAATGCCACCGACCACCAACATAATGCCGTAGGGCACAAGAAAAGCGCCTAAAATTTCACATATTTTTAAATTAAATCTAGTCTAAGAGAACATACGGATTCAATAAAATTTGATTATATTTCAATGAACTGGAAGCTACGATTCCAAATCACAATCGTAGATATTCTCCGCATTACATCCGGTAATTTTTAACCAACTTATTTTCGAGCTGTAGAAACAATCAATATGTTTTCTATGATAATGATTGCATCATAACCATCACATATTGAGACTGACCCGTGTCACAATCTGTGCATTGTTCGCGCTGCCCCCTCATCGACAAACATAATTACACACACAGTTTCTATATAGACACACTCGACCTTTATGTATATGTTCGATGGCACACAAAATCAAATTGTTTTACAAGTGCCACTCTAAGGACATTTTAAAAACATTAACAGCAGCAGCAAGCTGGCGGGTGAGAGGCGGAATATGCGGGGAAAAACCCAACTCGGCTGTCTCCTGCAGTCATCGCCGTCATCTCCGTCACCTCCGGAAAAGCAGCCGAGCCAAAGTTACACGCCCCTTTTGGGCCAGACAATTGGTTAGAGTGCCATCGATACTTTGTCGAGTGGCAGGGGATGATGTTCTATGATTTGATTATGTCTATTAGCAGGCAAAGTGGCCGACGACAAAGTCGTCTAGGGCCAAAACCCACATACACCCAACTGTATGCAAGGGCGTTCGGTGCAAAGTATATCTACAAAGGGTCTGCCAGCTACGGTGCTTCCTTCTGT

CATCCATGACGCTCGTCTCTGTTGTTGAAGCCATAATTTCCGTTGCTTCAAGTGCATTTCCACCATTTTCCAGCAGCTCGTCGGAGACACTGTGGAAATATGTGCCTCTAATTCGATTCCCATCCCGTAATTCGGTCAAAACTTGTCCAATTTTAAACGGCCAAGTGTTGGTTATTGCTTGACTAGCAAATTGATATTCTATTGAATTATTGAAAGTTATGCCTACTTAAATGCTTGTGAATAACTTTTTACATGTATTTGTGTAGGACTATTCTGGTTTAAGCTGAGGTGCAAAGTCTCACCATTAAGTCCTTCAGTTCCAAATAGAAATCTAAACTTTTAGACAAATTTAAGAATAAAGAATATAATTTATTGAAATACATAGTTAACCCAAGTACAATCAAGTGCGTATGCGCGATCTTAGTTTGAAAAAAAGCCAGGCAGCTCCTTGAGTTTTAGCCCTAATCCAGAACAATGTGGATGGCACAGCCTGTTGCTGCCACCAAAGCTTCCACTTCCGAGTTGCAGGCAGCAGGAAAATGGGAAACAGAATAAATAATACCGACTAAGCTGTGGTAATAATCGCAACCTGAGCGAGTTGCTGCCCCTGGCAACGGACATAAAATGGAACTTAAGCTGAAGATGGAGCTGGAACCACTGATTGAACGCAATGAAGCGTATAAATATAACAATTTATTATTATGATTTTAAAGCAGCAGCAGCAGCAGCGACCGGGGGTTAGCAGGAGGAAAAAAAGAAAACTCATAAAATAGTAATAACTATATCGAAGGCCCTGTGTGCCACATCCATTCACGACGCATCAGCGGGAAAGTGCCGCAATCAGAGGGAAAATGGGGAGGTGG

 

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