CR00012

CRIMIC CR00012

Gene DAT

CG8380

Information

Donor Location Cytology FBti Stock number
pM142R:16562128..1656218353C7FBti019945878865

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAACCGTCTAAACTGAAGTCGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGCACCACGGCATATCCAAT ACAGAAGGAAGCACCGTAGC CATCCATGACGCTCGTCTCT CCACCTCCCCATTTTCCCTC
 
Amplicon 5' Amplicon 3'
AGCACCACGGCATATCCAATTCCTGTAAGTTAAAGTGTCTTATCCATGAAGTTCTTGTGATTAATTAGTTAGTAGTTAGTAATTACCTTTGAAGAGGGGCACCAAGCGACCCCAGCAGGTTATGGCACCCTTACGATTGTGCTGACCCAGGGCCAATTCCATATAGAACAGAGGAATGCCACCGACCACCAACATAATGCCGTAGGGCACAAGAAAAGCGCCTAAAATTTCACATATTTTTAAATTAAATCTAGTCTAAGAGAACATACGGATTCAATAAAATTTGATTATATTTCAATGAACTGGAAGCTACGATTCCAAATCACAATCGTAGATATTCTCCGCATTACATCCGGTAATTTTTAACCAACTTATTTTCGAGCTGTAGAAACAATCAATATGTTTTCTATGATAATGATTGCATCATAACCATCACATATTGAGACTGACCCGTGTCACAATCTGTGCATTGTTCGCGCTGCCCCCTCATCGACAAACATAATTACACACACAGTTTCTATATAGACACACTCGACCTTTATGTATATGTTCGATGGCACACAAAATCAAATTGTTTTACAAGTGCCACTCTAAGGACATTTTAAAAACATTAACAGCAGCAGCAAGCTGGCGGGTGAGAGGCGGAATATGCGGGGAAAAACCCAACTCGGCTGTCTCCTGCAGTCATCGCCGTCATCTCCGTCACCTCCGGAAAAGCAGCCGAGCCAAAGTTACACGCCCCTTTTGGGCCAGACAATTGGTTAGAGTGCCATCGATACTTTGTCGAGTGGCAGGGGATGATGTTCTATGATTTGATTATGTCTATTAGCAGGCAAAGTGGCCGACGACAAAGTCGTCTAGGGCCAAAACCCACATACACCCAACTGTATGCAAGGGCGTTCGGTGCAAAGTATATCTACAAAGGGTCTGCCAGCTACGGTGCTTCCTTCTGT CATCCATGACGCTCGTCTCTGTTGTTGAAGCCATAATTTCCGTTGCTTCAAGTGCATTTCCACCATTTTCCAGCAGCTCGTCGGAGACACTGTGGAAATATGTGCCTCTAATTCGATTCCCATCCCGTAATTCGGTCAAAACTTGTCCAATTTTAAACGGCCAAGTGTTGGTTATTGCTTGACTAGCAAATTGATATTCTATTGAATTATTGAAAGTTATGCCTACTTAAATGCTTGTGAATAACTTTTTACATGTATTTGTGTAGGACTATTCTGGTTTAAGCTGAGGTGCAAAGTCTCACCATTAAGTCCTTCAGTTCCAAATAGAAATCTAAACTTTTAGACAAATTTAAGAATAAAGAATATAATTTATTGAAATACATAGTTAACCCAAGTACAATCAAGTGCGTATGCGCGATCTTAGTTTGAAAAAAAGCCAGGCAGCTCCTTGAGTTTTAGCCCTAATCCAGAACAATGTGGATGGCACAGCCTGTTGCTGCCACCAAAGCTTCCACTTCCGAGTTGCAGGCAGCAGGAAAATGGGAAACAGAATAAATAATACCGACTAAGCTGTGGTAATAATCGCAACCTGAGCGAGTTGCTGCCCCTGGCAACGGACATAAAATGGAACTTAAGCTGAAGATGGAGCTGGAACCACTGATTGAACGCAATGAAGCGTATAAATATAACAATTTATTATTATGATTTTAAAGCAGCAGCAGCAGCAGCGACCGGGGGTTAGCAGGAGGAAAAAAAGAAAACTCATAAAATAGTAATAACTATATCGAAGGCCCTGTGTGCCACATCCATTCACGACGCATCAGCGGGAAAGTGCCGCAATCAGAGGGAAAATGGGGAGGTGG
 

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