CR00014

CRIMIC CR00014

Gene Liprin-alpha

CG11199

Information

Donor Location Cytology FBti Stock number
pM142L:6727054..672710727A1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band CTCAAGGCGGAGAGGAATAATACCAGG GACAGCACGGGCAAGTTGAGCTG

Images

Construct Design Sequences

sgRNA sequence ATTGATTAGTCACGCTTAAGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTCTGCCAAGGAGGAACTGG ATGAAAGAGCAGGCTAGGGC CCCCCGACAACTAATCGAAC GCTGCCGCTTAAAGTTCTCG
 
Amplicon 5' Amplicon 3'
CTCTGCCAAGGAGGAACTGGCCCAGTACAAGGCGGGCGTGGTGCCAGCTGGCGTGGGCAGTGGCAGCGGAGCAGGGAGTGCCGCAACCACCGCCGGTGGTGGGGGCGCCGAGAACGGGCTCAAAGAGAAGGTGCGTTCCACATTCGAAATGCAGTCAAGCATGTCCGGTTTAATATATCTCTTCTATTTTCCAATACCCTAGATGGCGGGCGTGGGCGGATCTGGTGGCGTTAATGGCGAGGCAAATGAGCTGAACGACTACGCAGCCAAAACCCACGAGCTGCAAACCATTATTGAGAAGCAGGTGAAGATTGCGGATCTGTTTCCAGTCTGCATATGGTGAACACTTTTTAAAACGCTCTTTCCTTGTCAGACCTCGGAACTTTCACAATGGCAGCGACGCGTTTCTGATTTGAACAACAAGATTAGTGAGCTTGAGGAGAACATGTCGCGAGTGCAAAAGGAACACTGCAAGGCCCAAGACCAGTGTGCCAAGCTGCAGCGGGATCTGCGCGAGAATGTTGCCCAGAAAGAGGATCAGGTGAGTGAATAATACTCACAGCATGGTTTCAGTTTTTTACTTATACGTTCATTTTTAGGAGGAGCGCATTACCACATTGGAAAAGCGATACCTGAATGCTCAGAGGGAGTCTACATCGTTGCATGATCTCAACGAGAAACTCGAGCAGGAACTACGGCATAAAGAGGCGCAGCTCAAGGTGGGTAAAACCAAGGAAATTACAGCTTATGGTTAAGGCAGGCATACATATCTTTACTTTTATTCATGAGGATCGATTTTTTTTTATTATATTTCGATAAGCGCACAGTCCATTTGTTCAAAAAAATCCATTGATTACTCATTACCTTACTGTAAAGTTATTTCCAATTGAATAGCAGATAAATTTTTCATGATCTTACAAGTCAAGCAATTATCGCTAGTATGCTAGAGTAATGAAATAAAATTAGCAGCGAAATCTAGTTCGTATAGCATCTGTGATATTTTGTAAAAAATCGATCCACTCTGTTTCTAATTGTGCCCTAGCCTGCTCTTTCAT CCCCCGACAACTAATCGAACAAACACCCGCCCCAAATACGAACCACAACCAATCCATGCCATATGATCAATGCCGAATCCGAATCAGCTGCACGAGGAGAAGATCGGGGCCATCGAGGAGAAGCTGGAGCTCTCCGAACAGAAGCTCGCCCAGCACGCCAAGCTGCAGCCCGACATGGAAGAGCAGCTAAAGGCACGCATGGAGGCATTGACTAAGGTAGGAAACAAGGTATGCACTCGAACTAATATGCGCGCCGGACAGGTTCCATATGACAAGTATCAACAACAAATATTATCCCTTACAACTTCTACTCTGTTTTGTGTAATGTTCTTATCTTTTGCGTATTTTCTGCCACAAGAAACCACCAACAACTTCAACAACAACTCAGTTTCTATGTACTATACCTACTATTTCTATCTTTGTACTGCACCAGAACTTTCTACTCTAACGCACCCGAAACCAAATCAAAATCAGACCTAACAATTCTCTCAGTATACCAATTACGAAGGTTTCCAATATCTCATATAATGTGGTTATATTTGGTAGATATCAATTCACGACATTATACATTGTCAGCATTTATTTCTTATGTTTTAATGTATTTGGATTGCAATGGGTACCTAAGTGTAGTTGGTATACAAGAGAATATACTCACTATTAGCTTTCATTACTAAAACCAGAACTAACATTTTCCTCCGCCACTGATTAAACCGTGTGGACAAGTTCATTAAACTCGCTGGCTGAAACCATTTGCAGGCTCAAGAAAGACACGGTTCTGCAGAAGACCGCATCCGGGGACTGGAGACGAACCTCGACGAGAAGACCAACGAGGTGGTAAGACTGAACCAGCGTCTGAAGATGAACGAGGAGCACAACCTACGGCTTTCCTCCACGGTGGATAAGCTGCTCTCGGAGTCAAACGAGCGTCTGCAAGTGCACCTGAAGGAGCGTATGCATGCGCTGGACGAGAAGAACGCCCTCACCCAGGAACTGGAAAAGGCGCGTAAGGTGGCCGAGGAGCTACACCACGAGAAGAGCGAAATCATGAAGGAGCTTTCGAAGACGCGCCTGGAGATCGAGAACTTTAAGCGGCAGC
 
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