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CRIMIC CR00017

Gene CycA

CG5940

Information

Donor	Location	Cytology	FBti	Stock number
pM14	3L:11832602..11832636	68E1	FBti0199459	78866

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCCACTCGAGACCGAAAGTCC	TACAATAGTAGTCAACCTCCCTCTGCAAG

Images

Construct Design Sequences

sgRNA sequence	TCTAGGTTAGTTAAGGTTCTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAACCACTTCCAGGAACCG	CCTTGCAATTACGCGCCTTT	GGTTAGGCGCAAGTAAGTGC	CACACTGTCGGCAAATGTCG

 

Amplicon 5'

Amplicon 3'

TGAACCACTTCCAGGAACCGCTGGCGATCGTTGCGCGGCGGAAGCTCCTTGACTCTTCCAGTTGGCGAGACTCCCGTTTCAGTGCCCACGCTGATATCACTGGACTGTATGACACCGAGAATGGAGCGGTCCACGGACATGGGGGACTGAACATCCGTCACGGACATGGGTGTTGAATCCAGATCGTAGTCTACCAGCTCCTTCTGCCCAGCACCGCTGAATGGAGTGAAAAAAAAAACAAACAAAAGTTTGTTTGCGGCTTACCAAGTGACAGATGGAAACTTACAATTTCACGTCATGGTTCTCTTTGTCGACTAGGGAGGCCAGGGATTTGCCGGATGGCGCAACCTGGGTATCGTTGTTGTCCTCATACACGGAGAAGGTCTTGAATTGCTCCACCACGGGCACCACATTCGATTTCTTGGCGCCGTACTCCACATTCTCATCAACATTAAGATTTCTGACGTCACGGAAGACCTGCTGGAAGGAACAGAAGACGAAGAAGACAAATTCAACAATATGGTCAACTTACTATATTAGGTAATAATTTTATATTAGGTAATATTGCAGTGGGATTATAAAAGTAAAAACACAAAGATAATTCCTTTTACATATGCATCCTTAATATAGTAAGCAAAAAAGAAATAAAATGATTATCCCCCCCCATAAATCGATCCAACTTTAAGTTGCGCGCCTTTCCCTGGCTTCCCGTATTGCTCGTGTGTGTGTGTATGTGTGCACCTATACGCATGTGTGCGTTGCATGTCGCAATTGCTTTTGCCCTCCTTGCTTCGCTTTGTTGTTGCAAATCATCAAAAAGGCGCGTAATTGCAAGG

GGTTAGGCGCAAGTAAGTGCATTGGTCGTTCTAAGAAGAGAGTAACGTAAGATGGAGAAGTGAAAAGGATGGGATGGTAGGGGAGAGGTAAGGGAGAGAAAACCACCACCACCACTAGGAGATCTCACACATGCGCACGGTCGCAGTGGTGTGACGATCCCACCACCATAAAAATATAAAAAGGCAGCTGCATTGGAATTAGGCACTGGATGTACGGGAAATCGGCAACGGTACTTACAACTTTCCCGGCCGGACGCGGCACATTGTTGTTGCCATTGAGCACGGCGAAATTGGCCCGCGGCGCAAGCGCATTTTTCTCCGCTTTTCCCCCAATTACGGCCAGCGGCTGCTTGGTGTTCTTCACTCCGGCCGGAATCTTAATGCCCGGATTCTCCTTGTTGCTCATGTCTTGGTGGATCTGGAAACTGGCCATGGTGCGCTCTTTCCTACTCCTTTTTTCGCCTTGTAAAATGGAGTCCGATTATGCTCTGCTCTTCCCTTCCTTCTCTCTTTCTCTTAGCGTCGTTGCCTTCTTGCTGTTGTTGTTACTGCTGCTGCCGTTGTTGTTGTTTTGTGCTGCTTTCTTATGCTCGACGCTCACACCGATACGCACACATTCACAGGCTCATATGCTCAACGAAGACGCAGACATGTGTACATTAGGCGCAGGCAAATTTTATCATCGACAAATTGACGCTTGAATTACACGCACACACACAGTTTTGTACAGAATTCATTTGACTTTCAATTTCGTACGGAAGTTTGTGTCACGGCAGCGTTGGAATTAGTTTCCTTGTTTGGCTACGCTCGCACGGACTTAGCTAATATTAAGTATTTTACAAACTCCGTACTTGTTCAGCGGTTCGTGGTTTAAAAGCAATTGCTGGCTTCTTTTGATTTTTTCCCGGTCAACACGTCGTAAATGTTAGAGATGACACTGTCCAGCTATCGATAGGTCGTGGCGTCTATCGACATTTGCCGACAGTGTG

 

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