CR00017

CRIMIC CR00017

Gene CycA

CG5940

Information

Donor Location Cytology FBti Stock number
pM143L:11832602..1183263668E1FBti019945978866

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAGCCACTCGAGACCGAAAGTCC TACAATAGTAGTCAACCTCCCTCTGCAAG

Images

Construct Design Sequences

sgRNA sequence TCTAGGTTAGTTAAGGTTCTAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGAACCACTTCCAGGAACCG CCTTGCAATTACGCGCCTTT GGTTAGGCGCAAGTAAGTGC CACACTGTCGGCAAATGTCG
 
Amplicon 5' Amplicon 3'
TGAACCACTTCCAGGAACCGCTGGCGATCGTTGCGCGGCGGAAGCTCCTTGACTCTTCCAGTTGGCGAGACTCCCGTTTCAGTGCCCACGCTGATATCACTGGACTGTATGACACCGAGAATGGAGCGGTCCACGGACATGGGGGACTGAACATCCGTCACGGACATGGGTGTTGAATCCAGATCGTAGTCTACCAGCTCCTTCTGCCCAGCACCGCTGAATGGAGTGAAAAAAAAAACAAACAAAAGTTTGTTTGCGGCTTACCAAGTGACAGATGGAAACTTACAATTTCACGTCATGGTTCTCTTTGTCGACTAGGGAGGCCAGGGATTTGCCGGATGGCGCAACCTGGGTATCGTTGTTGTCCTCATACACGGAGAAGGTCTTGAATTGCTCCACCACGGGCACCACATTCGATTTCTTGGCGCCGTACTCCACATTCTCATCAACATTAAGATTTCTGACGTCACGGAAGACCTGCTGGAAGGAACAGAAGACGAAGAAGACAAATTCAACAATATGGTCAACTTACTATATTAGGTAATAATTTTATATTAGGTAATATTGCAGTGGGATTATAAAAGTAAAAACACAAAGATAATTCCTTTTACATATGCATCCTTAATATAGTAAGCAAAAAAGAAATAAAATGATTATCCCCCCCCATAAATCGATCCAACTTTAAGTTGCGCGCCTTTCCCTGGCTTCCCGTATTGCTCGTGTGTGTGTGTATGTGTGCACCTATACGCATGTGTGCGTTGCATGTCGCAATTGCTTTTGCCCTCCTTGCTTCGCTTTGTTGTTGCAAATCATCAAAAAGGCGCGTAATTGCAAGG GGTTAGGCGCAAGTAAGTGCATTGGTCGTTCTAAGAAGAGAGTAACGTAAGATGGAGAAGTGAAAAGGATGGGATGGTAGGGGAGAGGTAAGGGAGAGAAAACCACCACCACCACTAGGAGATCTCACACATGCGCACGGTCGCAGTGGTGTGACGATCCCACCACCATAAAAATATAAAAAGGCAGCTGCATTGGAATTAGGCACTGGATGTACGGGAAATCGGCAACGGTACTTACAACTTTCCCGGCCGGACGCGGCACATTGTTGTTGCCATTGAGCACGGCGAAATTGGCCCGCGGCGCAAGCGCATTTTTCTCCGCTTTTCCCCCAATTACGGCCAGCGGCTGCTTGGTGTTCTTCACTCCGGCCGGAATCTTAATGCCCGGATTCTCCTTGTTGCTCATGTCTTGGTGGATCTGGAAACTGGCCATGGTGCGCTCTTTCCTACTCCTTTTTTCGCCTTGTAAAATGGAGTCCGATTATGCTCTGCTCTTCCCTTCCTTCTCTCTTTCTCTTAGCGTCGTTGCCTTCTTGCTGTTGTTGTTACTGCTGCTGCCGTTGTTGTTGTTTTGTGCTGCTTTCTTATGCTCGACGCTCACACCGATACGCACACATTCACAGGCTCATATGCTCAACGAAGACGCAGACATGTGTACATTAGGCGCAGGCAAATTTTATCATCGACAAATTGACGCTTGAATTACACGCACACACACAGTTTTGTACAGAATTCATTTGACTTTCAATTTCGTACGGAAGTTTGTGTCACGGCAGCGTTGGAATTAGTTTCCTTGTTTGGCTACGCTCGCACGGACTTAGCTAATATTAAGTATTTTACAAACTCCGTACTTGTTCAGCGGTTCGTGGTTTAAAAGCAATTGCTGGCTTCTTTTGATTTTTTCCCGGTCAACACGTCGTAAATGTTAGAGATGACACTGTCCAGCTATCGATAGGTCGTGGCGTCTATCGACATTTGCCGACAGTGTG
 

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