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CRIMIC CR00018

Gene Got2

CG4233

Information

Donor	Location	Cytology	FBti	Stock number
pM14	2L:1986406..1986412	22B8	FBti0200712	81131

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGCGTCTTATCAGGCTATTCTTTGTTCAGAAG	CGATGATGCGGTCGGCC

Images

Construct Design Sequences

sgRNA sequence	GGGGTACGAAGTGTTAACAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCACAGGTGTCCTTGTCGTA	TGGGGGCGAAATCCAAGAAA	AACACTTCGTACCCCCAACG	ATTTGTTTGTCCTTGCGGGC

 

Amplicon 5'

Amplicon 3'

GCACAGGTGTCCTTGTCGTAGTAGCGGTATCGGTTCACCGGCAGACCGGCGTGCTCGAAAATGGCCACATGGTTGCCCCACGATGGCGACGGGATGTAGATCTCGCGGTTGCCCTGCCAGAACTTGGCCAGGAAGGCGGCTCCGATGCGCAGAGCTCCAGTTCCACTGATGGACTGGGCGGTCACGTTGTGCTTGGCCGCCAAACGCTTGGATCCCTGTAATAAAGGAATACTTATTAATTTTTAGGACATTCCTCAGTCATAAGGGATATAATTACCTTGCCCAATGCCAGCTCGATGGCCTTGTTGTAGAACTCGGGAATGCCGATGATTGTGGCGTACTCCTTGTCCAGACTACGGCTCACCACTCTCTTCTCGGCCTGGTCATAAACGATAACTATGTTTCAAAATTGGGTTTAGTTTAAACTTGATCTGAACTCACCTCCCGAACACTGGGGAGCACGAAGGGCTGGGTGTTGTCATCGCGATAGGCGCCAGCGCCCAAGTTGATCTTCTTGGGGTTGGTGTCCTTCTTGAAGGCTTCCGTGACACCCAAGATGGCATCGGGTGGGCCCATCTGCACCTCGGAGAACCATGTTGATCTAGAATAATAATGATATATTAATCATTGCATCACAATATCCATCACGTATGTATTAAAAACATCCTTATATCTGCCACACTTGCCAAGTGATTTCTATTGCCCGTCAACCTTTAACCCTAGAATGCCCGAGAACGCCCCACGAAGTCCTTATCTGTTTTAAATTTAATTGGTAGATTATGAAACTGCTTAGTAGTAGGTTATCTCATCCACGTCTGACCACTTGTGGCTATCATTTATCATTTCGCTTTCATGACTAAAGTTTGAAAGTCATCTAAAGGTTTTCTTGGATTTCGCCCCCA

AACACTTCGTACCCCCAACGACACGGGGGTCTTAAATAGCCCCCCCATATCAGCTGTAAACATGTTATTATGGGAACTCGGTCGGCGATTACAAAGTATACAATATAGATCCATTTAAGCCTACGTCAGCGATAACCTCGATGATTGTTTGCTGACGTAGCCGGCTTTCCAAATCTCCTTCTCAATATTCAGCAATAAATCGTGGAAGTTATTAAACTTAATTAAACTCTTTCAATTAGGCGCCACCTGTCTTTCGATCTGATTGTTTGATTTCCGGAAAGAACCTTCTGACTTCGATAAAACAAACCGCCCACAAGCCAAGGAGCTTCATAAAATGGAAATCAAAGCACGCGACAGAAGCGTGATATGACCACGAATACCCTACACTCTGTTCGCTCGGCACATCCGTTCTCCCGGGTCGTTGGACCACACATGCACAATGTCCTTAAGCGTCATAATAATGGTTCTACTCATAACTTAATATTAACTTAAACTTGCAATTTTGATGTAGAAAAAATACTTTTTGATGTCTTTTTCACAGTTGATTTCGTACAAATTCAAATATTTTGAAACTTGAATTATTTCTTTATTCCTAACCACTAAAAATATAATAATTCAAGAATGTTGGAGTGATTTAAGCTCATTTTGGCACCTGTTTTCTACCAACCCATCCGTCGTCATGTGGTTTCAAAATGTTCCCTCAAGTGGAATGAAATTAGCAGAAAAGAGCATGGAGCGTCACAGAATTGGCTTTAAAAATAAATACACATACGTCCGCCAATAGGCCAATCAGTCTATATAGTATAGTAGAGGGCCCGAAACATATGCAAATATTAGCCGACGAGATAAAAACAGCTGGAAACAGCGGAAATAATTGTTTTACATTTGCCCAGAGCGAAAGATGATTAAATAAAAGGCCCCTGTGCTGATATAACAATAAATCCGTATAACAGATTTAGTATAGTGCTAAAACTGGAGAACGAGCCCGCAAGGACAAACAAAT

 

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