CR00018

CRIMIC CR00018

Gene Got2

CG4233

Information

Donor Location Cytology FBti Stock number
pM142L:1986406..198641222B8FBti020071281131

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK GGCGTCTTATCAGGCTATTCTTTGTTCAGAAG CGATGATGCGGTCGGCC

Images

Construct Design Sequences

sgRNA sequence GGGGTACGAAGTGTTAACAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCACAGGTGTCCTTGTCGTA TGGGGGCGAAATCCAAGAAA AACACTTCGTACCCCCAACG ATTTGTTTGTCCTTGCGGGC
 
Amplicon 5' Amplicon 3'
GCACAGGTGTCCTTGTCGTAGTAGCGGTATCGGTTCACCGGCAGACCGGCGTGCTCGAAAATGGCCACATGGTTGCCCCACGATGGCGACGGGATGTAGATCTCGCGGTTGCCCTGCCAGAACTTGGCCAGGAAGGCGGCTCCGATGCGCAGAGCTCCAGTTCCACTGATGGACTGGGCGGTCACGTTGTGCTTGGCCGCCAAACGCTTGGATCCCTGTAATAAAGGAATACTTATTAATTTTTAGGACATTCCTCAGTCATAAGGGATATAATTACCTTGCCCAATGCCAGCTCGATGGCCTTGTTGTAGAACTCGGGAATGCCGATGATTGTGGCGTACTCCTTGTCCAGACTACGGCTCACCACTCTCTTCTCGGCCTGGTCATAAACGATAACTATGTTTCAAAATTGGGTTTAGTTTAAACTTGATCTGAACTCACCTCCCGAACACTGGGGAGCACGAAGGGCTGGGTGTTGTCATCGCGATAGGCGCCAGCGCCCAAGTTGATCTTCTTGGGGTTGGTGTCCTTCTTGAAGGCTTCCGTGACACCCAAGATGGCATCGGGTGGGCCCATCTGCACCTCGGAGAACCATGTTGATCTAGAATAATAATGATATATTAATCATTGCATCACAATATCCATCACGTATGTATTAAAAACATCCTTATATCTGCCACACTTGCCAAGTGATTTCTATTGCCCGTCAACCTTTAACCCTAGAATGCCCGAGAACGCCCCACGAAGTCCTTATCTGTTTTAAATTTAATTGGTAGATTATGAAACTGCTTAGTAGTAGGTTATCTCATCCACGTCTGACCACTTGTGGCTATCATTTATCATTTCGCTTTCATGACTAAAGTTTGAAAGTCATCTAAAGGTTTTCTTGGATTTCGCCCCCA AACACTTCGTACCCCCAACGACACGGGGGTCTTAAATAGCCCCCCCATATCAGCTGTAAACATGTTATTATGGGAACTCGGTCGGCGATTACAAAGTATACAATATAGATCCATTTAAGCCTACGTCAGCGATAACCTCGATGATTGTTTGCTGACGTAGCCGGCTTTCCAAATCTCCTTCTCAATATTCAGCAATAAATCGTGGAAGTTATTAAACTTAATTAAACTCTTTCAATTAGGCGCCACCTGTCTTTCGATCTGATTGTTTGATTTCCGGAAAGAACCTTCTGACTTCGATAAAACAAACCGCCCACAAGCCAAGGAGCTTCATAAAATGGAAATCAAAGCACGCGACAGAAGCGTGATATGACCACGAATACCCTACACTCTGTTCGCTCGGCACATCCGTTCTCCCGGGTCGTTGGACCACACATGCACAATGTCCTTAAGCGTCATAATAATGGTTCTACTCATAACTTAATATTAACTTAAACTTGCAATTTTGATGTAGAAAAAATACTTTTTGATGTCTTTTTCACAGTTGATTTCGTACAAATTCAAATATTTTGAAACTTGAATTATTTCTTTATTCCTAACCACTAAAAATATAATAATTCAAGAATGTTGGAGTGATTTAAGCTCATTTTGGCACCTGTTTTCTACCAACCCATCCGTCGTCATGTGGTTTCAAAATGTTCCCTCAAGTGGAATGAAATTAGCAGAAAAGAGCATGGAGCGTCACAGAATTGGCTTTAAAAATAAATACACATACGTCCGCCAATAGGCCAATCAGTCTATATAGTATAGTAGAGGGCCCGAAACATATGCAAATATTAGCCGACGAGATAAAAACAGCTGGAAACAGCGGAAATAATTGTTTTACATTTGCCCAGAGCGAAAGATGATTAAATAAAAGGCCCCTGTGCTGATATAACAATAAATCCGTATAACAGATTTAGTATAGTGCTAAAACTGGAGAACGAGCCCGCAAGGACAAACAAAT
 

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