CR00019

CRIMIC CR00019

Gene KrT95D

CG5405

Information

Donor Location Cytology FBti Stock number
pM143R:24004228..2400428695D5FBti020071381132

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK GATGAAGGACAAGAGTTCGTCACGGG TATTGAACACAATTGAAAGTTAATTCAGCAAAGCTACG

Images

Construct Design Sequences

sgRNA sequence ACAGGTGTAAAATTAGCCTGCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CGCCCACAAACTTAACGCAA GGCTGGCTTTTTATCGCAGG TACACCTGTGCTACGGATGT GTCATCGAAACTGTGCTTAGCC
 
Amplicon 5' Amplicon 3'
CGCCCACAAACTTAACGCAACGCAACGCATTAGAAAGCCAAACAAAATTGATAGAACAAAAGTCGTTTAGTCAAACTCCAGTTAATAAGCACATCAATAAAAATGCAAAATCAAATCCGCCCGCATTTAAAATATATCCGCCCCGTACTCACCAGCTGTTCCGGTTCCGGGTCCAAGGCCACCGCCCACCAATTGGCTCAGATCTCCGCCGTTTCCGGTGATGTTGTCGTGCAACATAATCCGCGAGTTGGTGAAAAAGGGACGCAGCGATGGCTTCGGCGTGGAGCCCACCGATATGCTGTCCATGTCCGGTCCGGAGTCGTCGCCCTCGCAACAGGACAGCGACTCCAGCTCCTGGAAGAGCGCATCCAGGTCGCGTTCTCCGCGCAGCGCCGCCGTGCCTCGATGACCGGACTCTCCCTCCAGCTCCTCGCTCACCTTGAACCGCTTCAAAAGTGCCACGATCTTTTGCTTGAAATTTCGCTGCTGCAGCGAGATCGTCTAAAAGGAGGAAAGACCATAAAAATTATTATTAATATTTGCTTTTATTGCTTCAATTTTGTAACTATTAAGGGAAAGGGCTAAAAATATTAAACTGCACCAGTAGTTGTCCACTAGAAGCTACAAGATCTTGATCATAAGGAAACAAGATCTTTATATTAAGTTGAAAATATTGTTTTCCCTGCTCTTGCCTTTCCTCATTAATACCCCTTAATTGCCGCTGGATCAATCTAGAGTTGCTTATGGGTCATTAAACGAAATCTCAAGGACTGCGACAGCGTTGACGACTTGTGGGGAGCTGTTAAAATTTATGCATCGTTCTTTTTTAATTGCCCAGCGCTCATCGGTTTCTTTTTGTTTGTTTGTTTTATTTTTTAGGTTTTTTTTTTGTGCTACGGCCTGTCATTAGTCGACTTTTTGTCTACGCCAGCCATTCAAAATGATCCTGCGATAAAAAGCCAGCC TACACCTGTGCTACGGATGTTGGCCAGGTGCGTAGATAAAAAGGGGCGCAGGGAGGCAATGGGCGGGCACCAGGACGAGGCAAAAGTCCATAATTGGCCCAAACGGCTGAGCCGAGTGAGTCAAAGCCAAACGCTGTTTAAATATTTAAGCGGCAGCTTAAGAAAATTGTCTTTGTTGTGATTAGTACGAATGCTTTTAAGGGGAAAAAGGCATAAATAAAGCATGGAATTTCTAAATATGCTGTTCGCTTTGAAAAGAAAGTGGGGAAAATAATCCCATTTAATAATCCTTTTTCGTAGTACAAATCCCCCTTTATATCTAGTCCAACTTGGATATATTCTTATGCGGAAATCCACGACCCCGATATATAGCCCTGAGATATAGCCATAGATTCGAAAGTATGCCACCGGGCGAATCTAAGCAAATTGAGTCGAGTGAGCGACACGCCAAGGCCGCCGCCCCAACAATTGTCAACTATTTGCCACAGAAATTCTTGGCCACCCCGTTTGGGGCATGCTTTTCCCCGAACTTAAGTATCTGTGCAAATGTATGTATATGCACTCTTTTTCTTCTATACTATGGGGATACATATTTGATACATATTTCATTTGATTTGGCCGCCGGGTTGGTGGTAGTGGGTCTTTGTTTTGGGTCGGCTTTTTGTATCTTGTATCTGTGCCTGCGGATCTGGGTTAGCAGCAAGGAACTGACAATATTTCGCAATTGGCAAATGCCTTAGCAAGAATGAGGAGGTAGCAACAAGGTACTAACAATGTTAGACAAGGCTAAGCACAGTTTCGATGAC
 
Search Again