CR00019
CRIMIC CR00019
CG5405
Information
PCR Verification
PCR check relative to sgRNA sequence
HA-L |
HA-R |
Forward |
Reverse |
no band |
OK |
GATGAAGGACAAGAGTTCGTCACGGG |
TATTGAACACAATTGAAAGTTAATTCAGCAAAGCTACG |
Images
Construct Design Sequences
sgRNA sequence |
ACAGGTGTAAAATTAGCCTGCGG |
sgRNA2 sequence |
|
Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
CGCCCACAAACTTAACGCAA |
GGCTGGCTTTTTATCGCAGG |
TACACCTGTGCTACGGATGT |
GTCATCGAAACTGTGCTTAGCC |
Amplicon 5' |
Amplicon 3' |
CGCCCACAAACTTAACGCAACGCAACGCATTAGAAAGCCAAACAAAATTGATAGAACAAAAGTCGTTTAGTCAAACTCCAGTTAATAAGCACATCAATAAAAATGCAAAATCAAATCCGCCCGCATTTAAAATATATCCGCCCCGTACTCACCAGCTGTTCCGGTTCCGGGTCCAAGGCCACCGCCCACCAATTGGCTCAGATCTCCGCCGTTTCCGGTGATGTTGTCGTGCAACATAATCCGCGAGTTGGTGAAAAAGGGACGCAGCGATGGCTTCGGCGTGGAGCCCACCGATATGCTGTCCATGTCCGGTCCGGAGTCGTCGCCCTCGCAACAGGACAGCGACTCCAGCTCCTGGAAGAGCGCATCCAGGTCGCGTTCTCCGCGCAGCGCCGCCGTGCCTCGATGACCGGACTCTCCCTCCAGCTCCTCGCTCACCTTGAACCGCTTCAAAAGTGCCACGATCTTTTGCTTGAAATTTCGCTGCTGCAGCGAGATCGTCTAAAAGGAGGAAAGACCATAAAAATTATTATTAATATTTGCTTTTATTGCTTCAATTTTGTAACTATTAAGGGAAAGGGCTAAAAATATTAAACTGCACCAGTAGTTGTCCACTAGAAGCTACAAGATCTTGATCATAAGGAAACAAGATCTTTATATTAAGTTGAAAATATTGTTTTCCCTGCTCTTGCCTTTCCTCATTAATACCCCTTAATTGCCGCTGGATCAATCTAGAGTTGCTTATGGGTCATTAAACGAAATCTCAAGGACTGCGACAGCGTTGACGACTTGTGGGGAGCTGTTAAAATTTATGCATCGTTCTTTTTTAATTGCCCAGCGCTCATCGGTTTCTTTTTGTTTGTTTGTTTTATTTTTTAGGTTTTTTTTTTGTGCTACGGCCTGTCATTAGTCGACTTTTTGTCTACGCCAGCCATTCAAAATGATCCTGCGATAAAAAGCCAGCC |
TACACCTGTGCTACGGATGTTGGCCAGGTGCGTAGATAAAAAGGGGCGCAGGGAGGCAATGGGCGGGCACCAGGACGAGGCAAAAGTCCATAATTGGCCCAAACGGCTGAGCCGAGTGAGTCAAAGCCAAACGCTGTTTAAATATTTAAGCGGCAGCTTAAGAAAATTGTCTTTGTTGTGATTAGTACGAATGCTTTTAAGGGGAAAAAGGCATAAATAAAGCATGGAATTTCTAAATATGCTGTTCGCTTTGAAAAGAAAGTGGGGAAAATAATCCCATTTAATAATCCTTTTTCGTAGTACAAATCCCCCTTTATATCTAGTCCAACTTGGATATATTCTTATGCGGAAATCCACGACCCCGATATATAGCCCTGAGATATAGCCATAGATTCGAAAGTATGCCACCGGGCGAATCTAAGCAAATTGAGTCGAGTGAGCGACACGCCAAGGCCGCCGCCCCAACAATTGTCAACTATTTGCCACAGAAATTCTTGGCCACCCCGTTTGGGGCATGCTTTTCCCCGAACTTAAGTATCTGTGCAAATGTATGTATATGCACTCTTTTTCTTCTATACTATGGGGATACATATTTGATACATATTTCATTTGATTTGGCCGCCGGGTTGGTGGTAGTGGGTCTTTGTTTTGGGTCGGCTTTTTGTATCTTGTATCTGTGCCTGCGGATCTGGGTTAGCAGCAAGGAACTGACAATATTTCGCAATTGGCAAATGCCTTAGCAAGAATGAGGAGGTAGCAACAAGGTACTAACAATGTTAGACAAGGCTAAGCACAGTTTCGATGAC |
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