CR00021

CRIMIC CR00021

Gene Cdk1

CG5363

Information

Donor Location Cytology FBti Stock number
pM142L:10385743..1038577831D11FBti020071481133

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TAGATCGAATCAACAAGACCACGCCC AACTTTTCCGGCAGGGACACCTTAC

Images

Construct Design Sequences

sgRNA sequence GGAAGCCTGCGAATACTTTCTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTGGCGGAAATGCGATGAAC AGCCTGCGAATACTTTCTGGT TGTACACATGAGCATAAGTACGC TACATCGTCTGCTGTGTCGG
 
Amplicon 5' Amplicon 3'
TTGGCGGAAATGCGATGAACTGGATCGTAGATTAACATCTTTTGTATGAGATCAATACCATTCGCATCGAGATTCTTTAACTGATTGGTCAATTGGTTCGTGGACCAGCAGGGGAACGTGTTCTTATAGTCGGGTAGCGAAGTAACGCCCGGCCAAATGTCTTCGGTAGGTGTTTTCAGAATTCTGCAATCAATGAGTGTGAGTATCACAGGGAATAGAAAGTAAATTACCTGTGGTTACCTAAACATTCTAAACAACTGGTCAATTTCCGAGTCACCCTGGAATAGCGGCTTTCTCGTTGCCATCTCCGCGAATATGCATCCAATGGACCAGATATCGACGGGACAGGAATACCGGGGTGAACCCAGTAGCACCTCCGGCGCTCTGTACCACAAGGTAACAATCTCGTGCGTATAAATGCGCACCGGAATGCCAAAGGATCGGCCAAGTCCAAAGTCGGCGACTTTTATGAGGCCACTCTTGTCGATTAGTAAGTTCTGCGGCTTAAGATCACGGTGAAGTACTCGCCGACGATGGCAGAAAAGAATGGCGCTAGTTATTTGGTACAAATAGCTACGGACCAATTCACTCTCCATGTGCTTATCAACTGGCAGCGAATCCATGTATTTCTTGAGGTCCATCGATAGGAATTCAAAGATCAAGTATATGCGGTTCTCCTCCATCAAAACATCCTCCAAACAGACAATGTTTTCATGTTTCAACTCCTTAAGCAACGAAATTTCTCTGTAATGGATATTAAATATTACTACATTATAGAAAAAACATGTGATTAAAGTATTTTAGTTATTGGTCTATCAGTTGAACCAGAAAGTATTCGCAGGCT TGTACACATGAGCATAAGTACGCATATAGGATTGTACATACACACACATATGTATGATGAACATAAGCCGTAACATAATTATGCTAGACTTCCACAGTATAAATTAAAAACAATATTGCAAAATCTTCAAACCAACCCATAAGATGTCAAGGAACATACTTTCAATTAAGCATATAAGCCATTTGAATGGAAAAGTCGCGAAAGAACATGAAACATTTTGAGGCTTGGGCGTCCAAGCCGCGGCATTTTACCTGATCGCGGTTGATGGAACGCCTTCGTCGTCGGACTCCAAGCGGATTTTCTTCATTGCCACAATTTGGCCCGTCAGGCGGTTGCGACCCTTATACACCACGCCATATGTGCCCTCGCCAATCTTCTCAATTTTCTCAAAATCCTCCATAATTTATTAAGCTATTTCTTTGCAAGCCACCGACTTTTAAATTAGCGCGTTTTTGACGTATATGCTCTAGTATTACCAGACAGTTTAAACGATGCTAAGCTAGCTACATTTATTCATTTGGAATGTGGGAATGAAACTCAATATTCAGGCAACTTATTGAATTGCTGTATCTTAAAAATGTTGTTAATTTTTTTAAAGTTAATTTTAGTGTACTGTAAAATTCCACGCTTTTTTTTATAGTGATTTCAATTAATTAAGACATCACTTTGTTTGAGTTCGGAGACAAACGTTTCTGCTAATATACTATTAATTAACTTATGAGTTTTTGGTGCAATTAATTTCTATTTTATATCTTTTGAAGGTGAGTACCAGTAGTACGGTACTAAAGAAATTAATTTAAAAAATGTAATTGGAATACCGCAACTGTTTTACTTCATTTTTGTTGTTTCAAAATTACTAAGCTCTTTGTCGATATCTTGACACACCTATATGCATGCCGCAAATGTGCATAACATATATATAACATAGTTTTTTTATTTTTTGCCGACACAGCAGACGATGTA
 
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