CR00023

CRIMIC CR00023

Gene ATPCL

CG8322

Information

Donor Location Cytology FBti Stock number
pM142R:15998358..1599836352D11FBti019946078867

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CGGCGCGTCAGTAAAAAAGCCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TAAAGTTCGCAATGCCCCCT TTTTACTGACGCGCCGAGAT CGGTTCCAACGATCAGCATC AGGCTCACCTCATTTGCGAA
 
Amplicon 5' Amplicon 3'
TAAAGTTCGCAATGCCCCCTCCGGTAATCAGCACTTTGCCATCGGGATGCTTTGGCGAGGAGGTCATCAGGTTGAGAATCGTCTTTGCGTACTCGTACGTCTGCTGTTCCGATGGAGCCCCACTGTATTCGCCATAATTGGCCAGCTCGGAGGCTCCACCCAAATCGCAGATGGTGTCCGAGTAAATGACACTGGCGCCACCGCCCGCCACCATCGTCCAGATACGACCATTACGATTAAGGATCGTCAGCTTCAGCGAAGCTCCACTCTTGGCGTCCAAGTCGGCGATGTAGGCCTCCTCGGGATATGCATCTCGACCAAACGGAGGTGGATAGTCAATCTCGCCCCATTTGGGACGGCAGATGAAATCAGCTGTCGAATCCAACTTGGCCGCCAAATCCAAGATGTACAAATTGTCGGCTGTCACCACCAGAGGGTTAATCTCCAGATATGTGAAATACAGGTCTACATAGGTCGTGTACAAGGCGCTGACAAACTTGGCAATGCGCTCCTTGGTGCCCGCGTCCTTGACCTCCTTCAACAGCTTGCTCTTCACGTCGGCTAACGACAGAGAAGAGTTCACAGGTACGTCCAACTTCACGGCCTTAGCATCCACATCGCCAATATCCACGCCACCCTGGTGGTAGAACAGAATGGTGTCTGCGGCGCGGTGCGAGTAGATGCACACGTACATCTCCTCAGCCTTATGGATTAGATACAATGGTGTTAAGTATACATACACATAATAAACAATTTGATAACTCACATCGGTGTGGGGCACAAAAGGTTCGATGATAAAGTTCCTGAGCTTTCCCACGGCATTTCCGATCTTCTGGTCCTTGTTGAGGCGCTCGCCAATCCACTGCTTCACCTGCTCAAAGTTCTTCTTGACGCCAATTAGTCCCAGCTTTCCACGGCGCTTGATAAGTTGATCGGGCTTGCACACCAGCGGCTGCAACAAAGGAAAGGACGGGGCTAATTAAATGTATGCTAAATATAAATGAAATAAGCGACAAATCGTTGAACTTGTGCCTTCTGATCTCGGCGCGTCAGTAAAA CGGTTCCAACGATCAGCATCTGTCGAAAAGATCAGCCGATAACATCAGGGGTCGTTCCCGCGTCATTGCCAATGACAATGCCAGTGCTCGTACCCTTACCCGAACTTGTACTTGTGCCCACACAAAAACACATCTCATATGCTTATTAGAAGATCGTACGAGTGTAAATTGAGCAAACAGTCCGACTACGAAATATCATCCGAGATGTCCGTCAGGCGATTCACTTTGTCGTGTAATTAAAGCCGAAAGAACTCCCATTATGCTCACTAAATTTCGGATCTATCTTAATTGCATATAAAATTACGAGATCAAAATATTAAACTAATAGCTCTAAGTGAAACTATTTAAAGGGAGTTGTATTGTGTTTGATTGTTTGCTTCTCCCCCTTCAGTGGGGTAAACATCATTATCAGTAACGTAATAAAAGTATTATGCACTTCAAACGAAAACGTGACAACAAGACAATAAGTCTATTAAATCAGAAAGTTAATACCTTCTATCTGCATGTCATAAATTAAAACACCTTCCTGATAATCAGAGTTTCACAATCCTGCCTTTAGATTACTAAGACTAAGTATAGGGACGCTCCCTCTAGAGTCAACAAACAACTTATAGATAAGTTTTAACTGATCACATTTCAATAGAATATATTATTTTGCGAAAATTGTTTGAAAGATAGTAGAGTAGTTCCCTGAATCCCCCACTCGACTCAGCGACACTTCGCCACTTTAAAATCTCCGGCATATTTGAATTGAAATTATTCTTTAGAAATTCAGGCGGCGCAACAATGCTAGGTAAGGTCAATAAATTGAGACGCTCCAAAATCAACAAATAACTTGATGATAAGATTTCGAACATATGGATTTCGGTGATAAAGGTTTTCTAAAACAAACACGGAACATGTTAGCTTCCCCTTATTTCCCACTCAACCGTTATAAGTGCTCAAGATAAGTACTTGGCTACAGCCAAACTTTCCACTATCGATCGGAAGGTCAATCAGAGATAGCTGCGTGATCGTGAATCCCAATCTCCACGCACGCTTATCGTGTTCGCAAATGAGGTGAGCCT
 

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