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CRIMIC CR00023

Gene ATPCL

CG8322

Information

Donor	Location	Cytology	FBti	Stock number
pM14	2R:15998358..15998363	52D11	FBti0199460	78867

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CGGCGCGTCAGTAAAAAAGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAAAGTTCGCAATGCCCCCT	TTTTACTGACGCGCCGAGAT	CGGTTCCAACGATCAGCATC	AGGCTCACCTCATTTGCGAA

 

Amplicon 5'

Amplicon 3'

TAAAGTTCGCAATGCCCCCTCCGGTAATCAGCACTTTGCCATCGGGATGCTTTGGCGAGGAGGTCATCAGGTTGAGAATCGTCTTTGCGTACTCGTACGTCTGCTGTTCCGATGGAGCCCCACTGTATTCGCCATAATTGGCCAGCTCGGAGGCTCCACCCAAATCGCAGATGGTGTCCGAGTAAATGACACTGGCGCCACCGCCCGCCACCATCGTCCAGATACGACCATTACGATTAAGGATCGTCAGCTTCAGCGAAGCTCCACTCTTGGCGTCCAAGTCGGCGATGTAGGCCTCCTCGGGATATGCATCTCGACCAAACGGAGGTGGATAGTCAATCTCGCCCCATTTGGGACGGCAGATGAAATCAGCTGTCGAATCCAACTTGGCCGCCAAATCCAAGATGTACAAATTGTCGGCTGTCACCACCAGAGGGTTAATCTCCAGATATGTGAAATACAGGTCTACATAGGTCGTGTACAAGGCGCTGACAAACTTGGCAATGCGCTCCTTGGTGCCCGCGTCCTTGACCTCCTTCAACAGCTTGCTCTTCACGTCGGCTAACGACAGAGAAGAGTTCACAGGTACGTCCAACTTCACGGCCTTAGCATCCACATCGCCAATATCCACGCCACCCTGGTGGTAGAACAGAATGGTGTCTGCGGCGCGGTGCGAGTAGATGCACACGTACATCTCCTCAGCCTTATGGATTAGATACAATGGTGTTAAGTATACATACACATAATAAACAATTTGATAACTCACATCGGTGTGGGGCACAAAAGGTTCGATGATAAAGTTCCTGAGCTTTCCCACGGCATTTCCGATCTTCTGGTCCTTGTTGAGGCGCTCGCCAATCCACTGCTTCACCTGCTCAAAGTTCTTCTTGACGCCAATTAGTCCCAGCTTTCCACGGCGCTTGATAAGTTGATCGGGCTTGCACACCAGCGGCTGCAACAAAGGAAAGGACGGGGCTAATTAAATGTATGCTAAATATAAATGAAATAAGCGACAAATCGTTGAACTTGTGCCTTCTGATCTCGGCGCGTCAGTAAAA

CGGTTCCAACGATCAGCATCTGTCGAAAAGATCAGCCGATAACATCAGGGGTCGTTCCCGCGTCATTGCCAATGACAATGCCAGTGCTCGTACCCTTACCCGAACTTGTACTTGTGCCCACACAAAAACACATCTCATATGCTTATTAGAAGATCGTACGAGTGTAAATTGAGCAAACAGTCCGACTACGAAATATCATCCGAGATGTCCGTCAGGCGATTCACTTTGTCGTGTAATTAAAGCCGAAAGAACTCCCATTATGCTCACTAAATTTCGGATCTATCTTAATTGCATATAAAATTACGAGATCAAAATATTAAACTAATAGCTCTAAGTGAAACTATTTAAAGGGAGTTGTATTGTGTTTGATTGTTTGCTTCTCCCCCTTCAGTGGGGTAAACATCATTATCAGTAACGTAATAAAAGTATTATGCACTTCAAACGAAAACGTGACAACAAGACAATAAGTCTATTAAATCAGAAAGTTAATACCTTCTATCTGCATGTCATAAATTAAAACACCTTCCTGATAATCAGAGTTTCACAATCCTGCCTTTAGATTACTAAGACTAAGTATAGGGACGCTCCCTCTAGAGTCAACAAACAACTTATAGATAAGTTTTAACTGATCACATTTCAATAGAATATATTATTTTGCGAAAATTGTTTGAAAGATAGTAGAGTAGTTCCCTGAATCCCCCACTCGACTCAGCGACACTTCGCCACTTTAAAATCTCCGGCATATTTGAATTGAAATTATTCTTTAGAAATTCAGGCGGCGCAACAATGCTAGGTAAGGTCAATAAATTGAGACGCTCCAAAATCAACAAATAACTTGATGATAAGATTTCGAACATATGGATTTCGGTGATAAAGGTTTTCTAAAACAAACACGGAACATGTTAGCTTCCCCTTATTTCCCACTCAACCGTTATAAGTGCTCAAGATAAGTACTTGGCTACAGCCAAACTTTCCACTATCGATCGGAAGGTCAATCAGAGATAGCTGCGTGATCGTGAATCCCAATCTCCACGCACGCTTATCGTGTTCGCAAATGAGGTGAGCCT

 

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