CR00026

CRIMIC CR00026

Gene rictor

CG8002

Information

Donor Location Cytology FBti Stock number
pM14X:19258507..1925852618B6Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK CAGCATAGAGAGAGCCCCCAAAAACG CGATGTGGTGACGGCCGCCA

Images

Construct Design Sequences

sgRNA sequence TCTAAATGAGTAGTTAAATCGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCGAAGCCTGTGAGTTGGAC ATCGGGCGAAACACTTTTGG CATGAAACGAGGTCAATGGCC AGGACTTCTACCGCCTGGAT
 
Amplicon 5' Amplicon 3'
TCGAAGCCTGTGAGTTGGACTCGTCGCTGGAGGAGACAGAATCCAGCCGAGAACGATCGAGGGTGCCGCGAAACTGAGTGACAGGCGGTACCGCAAATGCCGCCGCCTCCGCCGTTTCCTGCAACTGTAGCACAGGACCCGCTTGCTCCTGCACGTTGAGGTGCCGACGAAACAGTCGCGTCTGGATGAGAGCACCGCCCTGGATAATGCTGTCTAGGAATAAGCTGCGCGACGCCGGCCGCTGGCGCAGCAACTTCTGGTAGTTTTGCAGAGCGGCCACCGCTGCATTGGCCTGCTGGTTCCCCAATGTGGCATGACTCACCAGCGTGGGCAGTGCCGGACTCGTACAGCAAATATCCGGGGGCAGGTGGGTGTGCATTAGCTGCAGGATCTTGCCAATCAGCACCGTAGCCTGTACCGAGACGAACTGATCGCTGCTCACCACAACCTCGACGAGCGCATTGAGCAGTCCAGTCTCGAGGAAACAGTACAGCATCAGCGCCAGGTGCTGCTCTACTACGCTCGGTGCACGCGCCGCCAGCGTTGGCAAAATGGAGCGCCCTTCGGCGGACACGAAACCATTGCTCAGCAGCCACGTGTCCTGGAAGTCCGACGGATCCACAGCCTGCAACGCCACCGCATAGTCATCCGTCCAGGTTGGCTGCGGCAGCGTGAGCAGCTCGTACAGCAAATCCAATATTGCTTTCTGTTCAAATTGCATGTTCAGTAAGTTCAGATCGATACTAAGAGGGGAATGTACTCACTCTGACTTCGATCTGGTTCAAATACAGAGCGTCGACTATTGCTTTCAGGCCTGAGGGTTTGCTAGGATCACAGAATTCGAGGGTGCCCGTCCAGCTGCGCAGCACAGACAGCAGTGCCAGCCGACAGCTGGTGTAGCGAAGTTCGCGCGCATCCCTGAAAGATAGTTTTATTTATAAAAGAATGATGTAACTGATAGCGCTTATCTACAACTTGTAGTAGTACATCTTGTGAAAAAACTTCAGTAGATGTTTGTGGGATAGTCTTGTTCGATTTTCCAAAAGTGTTTCGCCCGAT CATGAAACGAGGTCAATGGCCGTGCTGCCTTTAGTTATCTATTTTAGAATTTCATGTTTGCATCCGCTGTTTGATAATCTCAAAAGACGGCGTGATGTTCATCGATTATTTGGTTACAATTCAACAGCAGATATAATTACTCTACATTAAATTACTTTCGGCGAATTAATGAGTGAAGTGGATCCGTCTTACATCTGGCTTGATAAACTGCTTTTTGTTTTGGGTAACCCATTTGTAGTATTCTGCAGCCAAGCTGCAGTATTCAAGGAGGGATTGGTGGGATCTTCACTAGGGCACTCACTTGTTCTTATCGACGATACTCTGCCGGTAGGTGAAATCGCAATAAGGAGCTGCCAGGCAGTCCAGCCGCACACCGCAGATGTTCCTCGTTTGCGGCCACTCGAGCAGGTACAGAAGCACGCCGCACAGGCTCTCAGCGATGCGGGGATTGTGGCACTCTAGCACATTGCGGGTTATGGAGGTGACGCCGCCGCAGACGATCAGCAGCGCCGGATTGAGCACAGCGAACTCTGCCAGGGTGGCCAGGCAGGCACGCAACAGATTGTCGTTCTCCTCGATGCCGCTATCCGCCAGCGAGACCAGGCAGCGCACCACCGCCGGACTGATGTCCTCCGGCGCGATGGCCAGCATCTTGCGGACCAGCTTCAGTGCCTGGACGCGCTCGTCGTCGTTCTTCAGCATTAGATCGATGGAGCGGCAGAGCAGGTGCTGCAGCTGCAGTGCATTGAACGTCTTGATGTCATTGGGCGTGCTAATGGCATACCGGATCGTTCGCAGTGCCGCCGCTCGCACTTGGGTGAAGGTGTGCACCAGTGAGGCACTAAGGCTGCAATTAAGGGCGATCAATTAGCCATGTTAAGAACAATGGTTTCCCCAACGGGCATCTTACCAGTAGAGCAGCTCCTCGGTGGTGAAGCGTATATTGCTGTGGTGGGAGCCCTTTCGGGCCCCCAAACAGAGCGAGGCCAGCGCGTTGAGCAGAAACAGGCGCTTCGTGTCCCGCGTCTCCTCCAAACAGAGCATCGAGTATATTTCGAATGCATTTTCGGCGGCCGATCGCTGTGGATCCAGGCGGTAGAAGTCCT
 
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