CR00027

CRIMIC CR00027

Gene Idh3a

CG12233

Information

Donor	Location	Cytology	FBti	Stock number
pM14	X:19521465..19521505	18C8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TACAGTTGTCGTCGTCGGAGCTCTC	TGGCATTATGGAGACGGCATTAGCTTAGT

Images

Construct Design Sequences

sgRNA sequence	TGGTTTGCAGGGTTGGTTAGCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCACTAACTCTGGCTGGGA	CCACCGATGCAATGATCTGC	GAGCGTCGGACGAGAAACAA	CGATTCTCTCTGGTCGTCCG

Amplicon 5'	Amplicon 3'
ACCACTAACTCTGGCTGGGATTACGACACTTACCCGGACGGGGGTCACGTCCACGGCCTCCCACTCGATGGGGACATTGGCGGCGGTGAAGATTTTCTGCACGGCCGCAGAGATCTCAGGTCCGATGCCATCGCCAGGGATGAGGGTGACTTTCTTGGTGCCGGAGGAGTAGGACCGGCTGGCGGCGGGAGTCGCGTTGACCTGGAAAATAGCAAGACCATAGGCGGGTTACAGAGTGATCTACAGAAATGGTGGCGAGAAAACGCATAGATGGGCAGCAGCAACAGCAGCAGCATCTAGCGGGATTACATAAGTGCCCATGCCAAACAAAAATCCCGTCCGCCCTACCCCCCGTGCCAAACAGTTCGTCGCGTTGAGGTTAGAGTGCACAAATGATTCCCACGGCGCTGATAAGGCCGCAAATCAGGGCCCAATCTCGGTAACTGATTTTTTCCCGCCTCTCGAGTAGGGGGTGGGATTTTCGAGGGTCCGAACCACCCGCCCTGCCCGCCGTTATCGATATCTCGGCCACGCAACGAGGGAGCGAGAGAGAGAGTGTTATGTAAAGTGAATGCGAATTCTAATGCTGTGCTCTTTTCGTTTTCGCCGGACGGACAGCTGATGTCCTGGCGAAGGAGGTGGTGGCCCCCACTGCGGATCCCGGATGGTTCCGTCGCTGCCGCCGCTGTTATGGAACCAACCTGGCTCACTGCTGGCGTCGCAGTCACCGCCGGAGCATCACGCGCTGCAATCAAACCCAGTTGATTGAGCTGCAAGTGTTTTGCGTAATGTGTTGTTGTCGTTGGGAATCGCGGAATCGCAGATCATTGCATCGGTGG	GAGCGTCGGACGAGAAACAAAATCGCAATGTTAGTGGTAGTTGGCCAGTACTTTTTGGCCAGCACGTAATCAACTGCTGTCCTGCAGAATTGGTTTGGTCGCTGTATGGTGGGGTTAAGAGGCGCGACGCGGGGCGGGTCAAGTCGCTTAAGCCTAAACGGGTCTTACAATTTTCTGTATGAATCTAGCAGCCATTGCGTTGGATCGGTTTTTGCACTGATTCCTGCCGTATATTATATAGAAAAAAGTCTGTCTTTTTAGTCTGCGCCCGCCGTAGTCGCCACCTTGCGGAAAACTGGCGAATCTGCGGTATATCGGTCGATAGGCATGCGGGGGCAGCGTCGTTGCCACCTGGACTGCGCTCAGATGGGGGTGGAATTACAAATCACCCATATCCCCGTATTTGTATGCTCTCGCTTGCATCAGTCACATAATTGCTGTACGAGCATCATATGTATGGCGTAAAACCACATTAAAGTTGGCTGTGAATAAACAGATGTCGAATATCGATGTCGTCTGCCCGAAATTCAGTACAGCCTGCCGTGTGGCATCCCTGTACAGGGCAAAAGCGAAATATCGGAGCGGCCTAATCGATAGTTTGTGTATCCCTTGCAACCGTGGCTGCTACAGCCACAGTTTAAATTAATTTGAGCATTCGCTCAGTTTTCTGACGTATATTCTAGAAAAAGTGCTGAGAGTGTCCATCAAAAAGGGGCAAGCGAAGCCGTTTCTCGATCTCCAACGAAAACGGGGATAGGATAATCCAAGGGGCCGCCTGTCGAGAAGCCAGGCGAGGGCAAAAAATCGGATCAACCGACAGACAAAGCTGAACTGCGGTCGTCGGCGACAAAGTGCGCTGTGCCCACGGACGACCAGAGAGAATCG

Amplicon 5'

Amplicon 3'

ACCACTAACTCTGGCTGGGATTACGACACTTACCCGGACGGGGGTCACGTCCACGGCCTCCCACTCGATGGGGACATTGGCGGCGGTGAAGATTTTCTGCACGGCCGCAGAGATCTCAGGTCCGATGCCATCGCCAGGGATGAGGGTGACTTTCTTGGTGCCGGAGGAGTAGGACCGGCTGGCGGCGGGAGTCGCGTTGACCTGGAAAATAGCAAGACCATAGGCGGGTTACAGAGTGATCTACAGAAATGGTGGCGAGAAAACGCATAGATGGGCAGCAGCAACAGCAGCAGCATCTAGCGGGATTACATAAGTGCCCATGCCAAACAAAAATCCCGTCCGCCCTACCCCCCGTGCCAAACAGTTCGTCGCGTTGAGGTTAGAGTGCACAAATGATTCCCACGGCGCTGATAAGGCCGCAAATCAGGGCCCAATCTCGGTAACTGATTTTTTCCCGCCTCTCGAGTAGGGGGTGGGATTTTCGAGGGTCCGAACCACCCGCCCTGCCCGCCGTTATCGATATCTCGGCCACGCAACGAGGGAGCGAGAGAGAGAGTGTTATGTAAAGTGAATGCGAATTCTAATGCTGTGCTCTTTTCGTTTTCGCCGGACGGACAGCTGATGTCCTGGCGAAGGAGGTGGTGGCCCCCACTGCGGATCCCGGATGGTTCCGTCGCTGCCGCCGCTGTTATGGAACCAACCTGGCTCACTGCTGGCGTCGCAGTCACCGCCGGAGCATCACGCGCTGCAATCAAACCCAGTTGATTGAGCTGCAAGTGTTTTGCGTAATGTGTTGTTGTCGTTGGGAATCGCGGAATCGCAGATCATTGCATCGGTGG

GAGCGTCGGACGAGAAACAAAATCGCAATGTTAGTGGTAGTTGGCCAGTACTTTTTGGCCAGCACGTAATCAACTGCTGTCCTGCAGAATTGGTTTGGTCGCTGTATGGTGGGGTTAAGAGGCGCGACGCGGGGCGGGTCAAGTCGCTTAAGCCTAAACGGGTCTTACAATTTTCTGTATGAATCTAGCAGCCATTGCGTTGGATCGGTTTTTGCACTGATTCCTGCCGTATATTATATAGAAAAAAGTCTGTCTTTTTAGTCTGCGCCCGCCGTAGTCGCCACCTTGCGGAAAACTGGCGAATCTGCGGTATATCGGTCGATAGGCATGCGGGGGCAGCGTCGTTGCCACCTGGACTGCGCTCAGATGGGGGTGGAATTACAAATCACCCATATCCCCGTATTTGTATGCTCTCGCTTGCATCAGTCACATAATTGCTGTACGAGCATCATATGTATGGCGTAAAACCACATTAAAGTTGGCTGTGAATAAACAGATGTCGAATATCGATGTCGTCTGCCCGAAATTCAGTACAGCCTGCCGTGTGGCATCCCTGTACAGGGCAAAAGCGAAATATCGGAGCGGCCTAATCGATAGTTTGTGTATCCCTTGCAACCGTGGCTGCTACAGCCACAGTTTAAATTAATTTGAGCATTCGCTCAGTTTTCTGACGTATATTCTAGAAAAAGTGCTGAGAGTGTCCATCAAAAAGGGGCAAGCGAAGCCGTTTCTCGATCTCCAACGAAAACGGGGATAGGATAATCCAAGGGGCCGCCTGTCGAGAAGCCAGGCGAGGGCAAAAAATCGGATCAACCGACAGACAAAGCTGAACTGCGGTCGTCGGCGACAAAGTGCGCTGTGCCCACGGACGACCAGAGAGAATCG

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