CR00030

CRIMIC CR00030

Gene Dhap-at

CG4625

Information

Donor Location Cytology FBti Stock number
pM143R:25488378..2548842596E2FBti020072081135

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band GGCTGGGAAACAGCACAACTGCTC TTGTTACTGAACTCCAGCTCCAAAGGGAT

Images

Construct Design Sequences

sgRNA sequence GAAAAGTGGCGTAAAATTAGCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CACATCCGCCTTAATGCTGC GTGACTGTCAACGGATTTGC AAAGTGGCGTAAAATTAGCGGA CGCCTTCACCTTTAACACGC
 
Amplicon 5' Amplicon 3'
CACATCCGCCTTAATGCTGCTCGGAGTCGTGCTAACATGGGCGCCCAGCTGCTCGAAGATTCGCTTCAGATGCAGAACACCTCGTGCTAGCTCGTCCAGAGTGACACTCCTTTGGGCATACAATTGACTGGCGTAGTAGAGGCTAAGCAGATTGAACGTGGAGATCACAATGCGACGCTGCTGCTGGTAGATAATCTCATTGGCCAGCTGTTTGACTAGTTCCACCTCCTGGCGGTTCAGCTTCTGCAAGTGACCACCCACGCCTGCTCGCTGCATTCGCTGCGCGCTGTCGTGTCCGAAAAACTCCTTGACCGAGATGGGCTCTCCGAAGTCGAGGAACATCTTGCCAAATCGCTCGTCGATGATTTTTAGGGCCTTGAAAAAGCCCTTTGTCGACTCCTTGGGCTTGGGCACACCCAGCAGCTCATAGACGAACAGTTGCTCCTCCAGGACGCGTTCGTAGGCCACACTCACTGGCACAATCATCACATCCGGCACTTCGCCCGTGAAATATGGCAACAGAGCCATCGAGAGTAGTCCAATTTTGGGAACCAGAGCTTTGAAATTCCTGGAGCGCGTGCCCTCAATGAAGAACTCCACGCCGATGTGGTAGTTTGCCACCAGCGCGTACATATACTCCCGGAAAATATCCCAGTATAGCTCATCGTTGGAGAAGCTGCGGCGCATGAAGAACGCTCCTGTTTTCCTCAACATGGTGCCCATTCCGAACATGGAGTGGAAATCTATAAAAATTAAGTAGACAACAATTAATATTATTGGGCAATTAAGAATATTAACGACTTTTGTTTAGAAACAGCGTACAAAAATTAGCAAATCCGTTGACAGTCAC AAAGTGGCGTAAAATTAGCGGAAAATATAAGCTCGCTTAATGTATTTAACACATTTTCCGCCAGATTGTAATTTGTTTATGGTTTTTGGCGAAACATACCCATGCCGGCTGCTATTCCTGGTATCTCGATGTCGTAGTAATAGCAGATGTAGGACATCAGGATGAAGTCCATGTAGCTGCGATGACTTGGCAGATAGAGCACCGGGCACTTGCCCATATCCTTGCGCACATTGGCCATGCTAGCCGAATTGACATAGAATCCATCGCAGATCCGCTTGCCGATCGCCGTAATGGCAATGCCGCACCACCGGATGATGGCCATATTCCGATCGAGGCCGATTTCATCAATGATCGCCCGGGCCTGACGCTCCATCTGCTTGAGCGGCGTGCCCGACTCCTTGGCATAGTGCTCCAGAATGGATCGCAGCTTCTCGCTCCTAAGGACGTGCTGCTTGAGCTTTTGCGGATTGAGATACTTTTCGAATTCGTATGCCACCTGCGGATTGAACTCCCTGGTCATGGAGGCCTCGTTGCCCGGCGCTATGATGTTCTTGAAGTTCCTCATATATTCGGCCGCACTGGGCGTCTTCTGCTCCTCCTGTTCGCTGGATTCACCTTCTACGGTGCCGGATGACGTCGACTGCCTCACGGGATTTCTATCCATCGCGCTGCGCTTCAACTGTGGAAATGGAAAAAAAAAAACGTGCGTCGGTCCGATGAGAATCTCTGGAGGTAGCTGACTGAAAGAAAATATTTGACGAATCAACTGTCAAGCAGGCGGCCCCACTTAACAGACATGACACACAAACACACTGCCATAAACTCGCTGTTTGCTGAGATATCGCAGGCACTGGGAGAAAATGTGTGCAACGAAATGTCGGAGTAAAAGATATATATATTTTTAATTTGTTAAATGTGCCATTTAAGTGTATATTAACTTTTTAAACACTTATTAAAAATTAATTTAACTTTCAAATTTGTATAATTTATAAAAAGAATTTAATTTAAATTTTGAAATATGCAGTGCATATCGAGTTTTATGTCTGATAGGTTATAGGTTCATTGCGCCAAGTGATCGTTTGTGTGAAGAGTGCCTTGCTATCCCAAAGGTTGATTATCTGACAGATTCATAAGCAGTTACGGCAATCAGCGGCGAGTGGCGAATGTTGCGTGTTAAAGGTGAAGGCG
 
Search Again