CR00033

CRIMIC CR00033

Gene Hsc70-5

CG8542

Information

Donor Location Cytology FBti Stock number
pM142R:14255476..1425547650E6FBti020072181136

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band CGATTGCTTGACAGACCCATTGGTAACT GCTTAGCAATCACGGGCTGAACGATG

Images

Construct Design Sequences

sgRNA sequence TGCTTATCTGGACTTGTTGCCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCAGCACATTAAGACCAGCG GGCGGCCTTATCAGTCTACT GCCGGCAACAAGTCCAGATA TGGTTTGCTCCAACTGGGTT
 
Amplicon 5' Amplicon 3'
GCAGCACATTAAGACCAGCGATTTGACCAGCATCCTTGGTGGCCTGGCGTTGGGAGTCGTTGAAGTAGGCGGGCACGGTGACCACGGCGTTCTTCACGGGAGTGTTCAGGTATGCCTCAGCGGTTTCCTTCATCTTCATCAGGATGAAAGCACCGATTTGGGATGGAGAGTACACCTTGCCGTCGGTGGAAGAGACCCAGGCATCGCCATTTGAAGCCTTGACCACCTTGTAGGACAGGTTAGTGATGTCTTTCTTAACCTCGGGATCATCAAAACGTCTGCCAATCAGTCGCTTGGTGGCATAGAAGGTGTTGGCCGAGTTTGTCACTGCCTGGCGCTTGGCGGGCATGCCGACCAGCCGCTCACCGTCCTTGGTAAAGGCGACGTGGGAGGGTGTGGTCCGGGCTCCCTCGGCGTTTTCAATCACCTTGGCCTGCTTGCCCTCCATGACGGCCAGGCAGGAGTTGGTTGTTCCCAGATCGATTCCAATAACAGCACCTTTGACCTCACCGGACCTGGAAAGGGGGAGGGGGTGGGAGGTTTGTTTTACATATGGATAGAGTAGACTGATAAGGCCGCC GCCGGCAACAAGTCCAGATAAGCACCACGTTTCTGGCCACAGATATTTGCAATTATCAAGGCATTTTCTTTTAAATATCGTACATTATGATGCAACATGAGGTAGCTTTTGGAAATCTATCTTATGTTTGTCTAGTGCTTACATAATTGTGTTCCGGTAATAAGAGAAATTTGCGAAATTATAACTCGATTTCACTTACTTGTAACGCAGCTGGGACGATATTCCATTGCTAGCACCTGGCAGATTCTGTAAAGACAAAAAGGGGCTCCGCTGTAGAATCTTCCCGCAAAACATGAGACCGCATCATCGGCATGCCGCACTTTATATAATAGTCGTTGGGTCGGGGCGGGGGGATGGTACTTACGCGGAACATGCTAGAAGCTCCGGACATATGGGAGGGTACCACGCCGGCCTGGCGGGCGAGACGGGGCAGAAACTTGGGTACGCGCAGCATTTTGGCTTTTTACTTCTGCAATTTCACACGAACAATTGTCAAATTAAAAGCAAAAATTTCGTCAGAACGGCGGAGCACGTTTCTTCCCGTGAGAGGACTGTAAGGTAAGTGGTGGACTCGGGCTTGTCTCTGTGCGACGGGCAATGCGATAGTTTCCCGCAACTCGCGTGCCAGAGTTGTCAACGGTGTGAAAGTGTTTCTTCAACGAATTGTGGTGTATAAAACTTTAAATATTTCAATGGCCCTTCAGCACAAGGTGGTTTGAAAACATAGTAAATGAAAGCAAACTAGATGTAAAGTGAAAAGAAACAGCAAAATTTTATATTCAAAGATTAGTTCGGATAAAATATTGTTTACTTTCTTAATTTTAATAAGATATTTATTTCATTAAAGTTATAAACTAAACAAGTGGCATCTAAATAAATAGCAATTGCAACACATTTAACATTTGTGATTTCCGATTAACCTACATATTTTTTACCACTGGTGTCGCTGTTGTAGTGAGACCGTACATCCGCAGTATGACCGTATCTCAAAACAAAACCCAGTTGGAGCAAACCA
 
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