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CRIMIC CR00036

Gene Itpr

CG1063

Information

Donor	Location	Cytology	FBti	Stock number
pM14	3R:5527959..5527980	83A8	FBti0200722	81137

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGACCACACAGCAATCAATTGGGTTC	CTTGTTCTGCTCGTTTTCCATTCCGGC

Images

Construct Design Sequences

sgRNA sequence	CCCAGGAGATCCATCACCTCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTTCAGGCGCCTTAACGGA	ACTTGTTGCGTCCTCGTTCA	ATCTCCTGGGGCCACCTATT	TTTCCTGCTCGGCATGGAAT

 

Amplicon 5'

Amplicon 3'

ATTTCAGGCGCCTTAACGGAAAATGTTTTGTGTGAAACAAAATAGGTTTTGAGAAATGTTGCTTGTTAAATAACCTAAATATAACCTAATATAACTCAAAAATGGATTATATTAAAAAAAAATAAGTTTCCCTAAAAAGACTATACTTACTATGAACTATGAACTCGGTTAATATTGAAAATATAAAGATATATAAAGATAAAGATACTACAGACGTGCAATTTTGTTACAGTTTTTATATTTAAATTTTCCGGTTACTGCTTTTATTTAAAACTCCAAGGAATCCAAAATTAAGCACATTACATAAATGGGCTAAGAAAAGGTCCAACTAATTCACTAATGGAATTGGATTATTTTTGAAACCATTAAGCGCTTAGGTCCATCATTCTAACTCTACGTGGTTTATTTTTCTATCCAACAGTAACGGTGCGTGTCGTCGCTAAGTGAACTGACGAGCTTTGTTTTCCCAATTCATATATTTATTATTCGAAAATGGCTTAATGGGCTAATATATTTTTTAAAAATATAACCATTTTTACAGTACACATATATGGCTAAAACATGCATTTATCGCACACTTTCATGTACGATAATCATACTGAGAGCGTTCTTTGTTTTTCCCCTAGATGTAAGCGAACTCACGTTGCTTTATCCTTTGCCAATCCCCGCAGGACATAACGAGGTCGACAAACCAGTGCAATTAACTCGCGTAGCCGACTGCGCCTCCAAACAATAGCTCCCAGCTCCGGAACAGCTACGGCCAGAGAGCACAGCAACAGGCGCTGGCACGTGGGGAGCATCGGGTGGAGGCCCATTGTTGCGCCACCTGGCGGCAGCCACTGTACACATTCGGCACTCTCGACTTGGATACCATTCCCATCGCAAGCCCCAACCCACAGTGGGCTTCAGACATGGGCGACAATATAATTGGCTCGGCAAGCTTCCTGCACTTGGGGGACATTGTGTCCCTCTATGCGGAGGGCAGCGTTTGCGGTTTCTTGAGCACACTCGGGTAAGTACCATTGTCCATGCTGAACGAGGACGCAACAAGT

ATCTCCTGGGGCCACCTATTGTTGAATACTGCACTAAACATCTGGTTTCTTTCGCCAGCCTGGTCGATGATCGCACAGTGGTGTGTCCCGAGGCCGGAGATCTCAGCTGTCCGCCTAAGAAGTTCAGGGGTAAGATGGGATATCTTATAGTTGATTTTCACTATGTTTATGATAAAAATTTTCAGACTGCCTCATCAAAATATGCCCCATGAACCGCTATTCTGCGCAGAAGCAATTTTGGAAAGCAGCTAAGCAATCGGCCAGCTCCAATACGGATCCCAATCTCCTCAAGCGTTTGCATGTAAGTTCAAGAATAGTTATAATATTTAATCGTTTAAAAATGTATATTGTGTTTCACAGCATGCGGCGGAGATCGAGAAGAAACAAAACGAGACGGAGAACAAGAAGCTGCTGGGCACCTCAATCCAATATGGACGAGCTGTGGTGCAGCTACTGCATTTGAAGTCCAACAAATATTTAACAGTTAACAAGCGACTGCCGTCGCTGCTGGAGAAGAATGCCATGCGCGTTTATCTGGATGCCAACGGGAACGAGGGGTCGTGGTTCTACATCAAGCCCTTCTACAAGCTTCGCTCCATCGGCGACTACGTGGTTGTTGGTGACAAGGTTATTTTGAGTCCGGTGAATGCGGATCAACAGAATCTCCATGTGGCAGCCAACTACGAACTGCCCGACAATCCCGGCTGCAAGGAGGTCAACGTTCTGAACTCGTCGACCTCCTGGAAGATCTCGCTGTTCATGGAACACAAGGAGAACCAGGAGCACATTCTCAAGGGCGGAGACGTGGTGCGTCTATTCCATGCCGAGCAGGAAA

 

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