CR00040

CRIMIC CR00040

Gene Acsl

CG8732

Information

Donor Location Cytology FBti Stock number
pM142R:8677435..867744044E2FBti020072581139

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK CTAGTGTTCTGGTGGCAGTCGTTCAG GCATAGACAGTGACAATGGGCATAGCC

Images

Construct Design Sequences

sgRNA sequence CAGGGACGCAATGTGACCTTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCGTGAAGGTGAGTGCTTTG GCCTGGCACTTCTCCCTAAG GGTCACATTGCGTCCCTGTA CGCGTACTTGGACTCATCGA
 
Amplicon 5' Amplicon 3'
GCGTGAAGGTGAGTGCTTTGACAGGACTTGCCACTAATCTGGCTAGTTGAAACGAATGGAAAAAGCCACAGACTTAGCCGGCCAATCGGTGCGACGAGGGGTGTAACTATCTCTAGAATCGTGTAGTTGAAATACTTTCGACTTTGCACTGCACGTTCCAAAGTACAGAGGCCTGCTACAATCGAGTTGTTTTAGTTTTTATTAAAAAAGTGCACCCTCGGTTATCAGTTTCATTAAGTTCTTTTCCGCATTTATTTCCCTTACTTCGAGCCTTGTTCTCGCCAGGTGGTTTCCTTATGTTCATTTCATTGTCTAATCTATGTAGATATGACTACTTTTGATTTATTGACCAGGTCCACCATTTCCACAATCTATTCGCCTTTATCAGGGCTTCGCTTTTTATGCGGTAATTATCATATCCGATTAACTTTCTTTCATTTTGCTTTATAACTCCGCGAATGTCAGGACTTCCAGCCATGGCTGTATTTGACAAGTGCACAGGTTGTCAGGCCATAATGAAATCGCAGTTGGTCCTGATAACAGAACCATGAAGTTAAGAAATCATAGAATATCTCTACCTTCTTTTCAAATGGAAATAATATTCACACATAGTGAGCTGTGCAAGTGAGACACCCTAGCTCTCAGCCCAGAAATAAAAAACATATTTCGAAGTGAGTGTTAGCTTATCACATTTCCTGCAAAATAAATTAGTCTTGTTAACTGAAAGCCTAAGGAATGGCGTTTAATAGCCGGGTTTTGGTGCCTGTCCTCAAGGCCGATGATGACGAAAAGGAGTTAGTTGATCCGCAAGCAGCCCTTAGGGAGAAGTGCCAGGC GGTCACATTGCGTCCCTGTACAACAAGTATCAAGAGTGCAATGATCGTGTGAATGGCAAGTCCAAAACCACTGAGACATGCATGGAGGAATTGTTCGACTTCGTCGCTGAATTGGATCATTGCGTTGCTCACAGTCTCTTCTCGAAACTCAAATAGTTTCAAATTCTAGCAATGCTAAAAAAATAAAATTGGCACTTAAATAAAGGAAACAAACTAAAACATAAATATTTCCAGCTATAACTCGTTTAGGAAAAGTCCCACCTGAGGTACACTTTTATCTGACTTTTCACTATCTAAATGGAACGCATTTTGGTTGGCCTTTTCAGTCGGCTGATAAGGTTTATGGGTTAGTAAGGGTACGCCAACGCCGGAATCTAAGATCGCTTTCATTTGTGTGCAGTTTGCATAATCTTTAGAAAAAATAAGTAAAACGAGTTGAATGCCTTAATCGGGAATCAGTAACTCTATGCAATACCACAGCTAACTAGCTTCTCATCGCATATCTCAACTCCTCCTTCCTCTACAGGCCAAGCCCATTAACCAGAAAATGTTGGTCGATGAGTCCAAGTACGCG
 
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