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CRIMIC CR00042

Gene L2HGDH

CG10639

Information

Donor	Location	Cytology	FBti	Stock number
pM14	2L:18973651..18973651	37B8	FBti0199461	78868

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGCATTTCATTAGCCGTTCTTCCAGG	CGATAGTGGCGAATCAGTACAGACATTAC

Images

Construct Design Sequences

sgRNA sequence	CCAAGATCTCGACGTTCTTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATACCAGTGTGCGGACTGTG	GCGGTCTGACAGTCGATGAA	AGACCGCAAGAACGTCGAG	CGCGTGCACATACTTATGGC

 

Amplicon 5'

Amplicon 3'

ATACCAGTGTGCGGACTGTGGAGCGCCATCACTCCCTGGCAGTAGGGTTCGATCTCCTGAATCTCGCTGCCTTCGATCATTCGCAGATCCGGTACGTTGTTGGCAATGCCACGCTTCTCCAAATCCTTCAGCAGCTTCACTTCTTTCTCATCCGTGGCCACAATTAGCTTCCCGGTTTTCTTGTACGGGATCTTTTTTTCATCCAGATAAGCGTAGGCCAAATGCATGCCCTCCACGCAAAGGCGGGCTTTCAGGGTTCCAGGCTTATAGTAGATGCCAGCATGGATGACGCCGGAGTTGTGGCCGCTCTGGTGCTTTGCCAGCTTGCACTCCTTCTCCAGGACAGCCACTTTGAGGGATGGATGGCGCAGGACAATCTCCCGGGCAGATGCGGCGCCGACGATGCCACCACCAACGACAACCAGATCATAATCTCTGAGGGAATACAAAACATATCAGCTAACAGCCTCAAAAATTAAATAAACTTAAGACTTCTAAAGTTTTTAAAAGTTAGAAAGATGTTTGTATATCCGTTAGAGTAAAGAGTGTGCGGGCACGTTTTTGATTAGGATCATCAGTCAAGTCGATCTAGCCATATCAATATGTTCATCGACTGTCAGACCGC

AGACCGCAAGAACGTCGAGATCTTGGGAACTAATAAGTAAGATAAAGGCTGCAGATTGCAGTGAAAAGACTATGCTATGCAATTAAGAATACAAAAGTAAATAGAATATACATTGCTTAACTAAACATGATTTTAAAAGCGCATTCTAAATGAAAAGAAGCTTACTTAGCTCATTTGGAACACCTAAATGATATTCTGCAAACAGTGAAAAGATTCTTAGGATCGGAACACCCCCGAAAATGAGAGAACCCTATCGTAATCTCAAGAGACTTACCCGCAGCTCGACGAGCTGTGCTGCGATCTTTTGTGGGTCGCTGTGGATTCGTTGGGCGCGGCCACGCCCACATTGAGTAGGCTACGCCGAAGTCCCTGTACCAGCAGTCGCACCTGAGCCATTTCTCTTTCTTTTATGCTTTCACCACCCGCGCACGCACGTAGAATTTAGATTATTTACCAACCCGAAAAGAAAGACCTTGCTTGACCTTGCCTTCTCCTCCGCCAACTTGTGCGTACAGCGCGCTTTCGAGCGAATCGACAGACTGAAGCCCGTGGAAGTTTTCCGTGGGGCGAGAGAAAATAAATATGCTCACGGGCCGAGCATCTGGGCGATTTGTGGGGCCGAGGGGCGATCATAAACGCGCTAATTGATAAGTGTGACGGATTATTTTTGAAAGGGGGTGGTTGGGTAAGGACGTGTGTGTCAGCTGTTCGGTTTGTTGATAAGATTTAATGTGCCCAGCTGCCATTTTGCAAATTTTCTGTAATCTAACAATATTAAAATTTTTATTTCATTTTAAATATTTCAGGTTATACGATCATGCTGATTACTCGACTAAAAAGTATTTACAAGAAACAAACATATAATTTTAACCCTAAATTTGCTTTTATTCGATACGGAATCCCATTATATTTGGTCGTATTTGATAGCAAACACAAATGAATAAAGTTTCGTTATTAATTCAGAACTGCAAGAAAAAAGACCGCTATCAACAATATGTGAGACGCATTTTCCAACACTGAACACTTCAGAAGCAAAACAAAACCAGCTGATTTAGCAAACAACAGCTAGCTCACAGATGTAAACAACAGAAATTTGCAAATTATTACAAATTATTGTACCATAAATACCTTAAGATGCCCGCCCACTGCGCCGTGATAAATTGCAGCCATAAGTATGTGCACGCG

 

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