CR00044

CRIMIC CR00044

Gene Uba1

CG1782

Information

Donor Location Cytology FBti Stock number
pM142R:9691972..969198146A1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band CGGTAAACTTGTGTTTACCAACACCGCC TTCTGCACATCATGCGGGCGGAACA

Images

Construct Design Sequences

sgRNA sequence AACCGAATGCGTTGAGCAAAGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CGTCGTGCGTGATACTAGCT GCTCAACGCATTCGGTTTT ATGCAAACACAACGGCAGC GGCGACGACGTTAGTACCTC
 
Amplicon 5' Amplicon 3'
CGTCGTGCGTGATACTAGCTATCATGGTGGAGATCGGCTGTGTGCCATCCTGATCGTAAATGGTAAAGCTCTCGCCAAAGTCACAGAACACCTTGGCGAACAGTCCGCGCGTCTCGGCGATGATCAGAGCAATGCCATTTTCATGGGCAAACTTGGCGATCCTCTGCTGCTCCTCCCCGTCGGAGTTGGTCAGTACCACAACGCGAAACTTGCGCAGGAACTCCTCGGTAAGCGGCCCCGTGTGCGAAACGGTGCGCACATAGTTGTTTAACTCGGCCAACTGGGCGCAAGAGGCCTCCGCACGATTCTTGCCGATATCGGCTTCCGTGAGATAGAATTGCGACGACAAGTCATGGAGCTGCAAGACAAACGAATAATCATTAATATTATATATCAACGGTGATGAAATTAGCAGACGGGAGCCATGACTAACAAAGGAAAATTGGTTCTGTGCCGAGTGTACAGCAAAGACATATTTGTCATTAGAACAAAAAAGTTTTTATGATACAAGAAAACTTTAAATTGAAAGGCTATTAACTAAATAATTGATATATTACGGTTTATGAGAAACTCATAGATCTTAAGTTGAATCTTTCAGTGCATAAAACAAAACCGAATGCGTTGAGC ATGCAAACACAACGGCAGCGAGCGATGCGCATACGGTTGTCCCGCTCACACACTTTTCGCATGGCGCAGACATTTTGAGGCAAATAGAAAAAGAAGGAAAACCGAATTACACACACAAAACGAATTTTTTGCAAGCAAATAGAGATGTTAACCTAACCTTATTTCGCGCGAAGTCTATGATAAATAAATCAAATAAAAACTGAATGATTCACTAAGACCAGGGATGCCAAGTCAGCCTTTCCGGGCATTTCCCGCGTCCGCCTGGAAAATTATCGATTTTTTCTTCGCCCCACACACATACCCCACAAGTTGCTGTGTCATGCAAGGTGATCGATTTCACGCCGCCCAGAATCACATTCTTGGCTATCTCCAGGCCGAGTCCTCCAAGTCCCGACAGAAGGATATCCGAATTGGCCATCCGACGCATCGCATCGTGACCCAGGACGTACAATTGGCGCGAGTACAGCGATTCATCGATATCTCCGCCGGCGGCGGCACTGTTTCCAGCCATGTTGCTGCTTCCTTCCAGTTGTCGGTTATTAGTGCTGTTATTACTGTTATTGTTGCTGGAGCTGACGTTGTTGTTGCTGTTGCTAGTGGCGGATGCACTGGAGGAGGCTTCTTGCGATTCTATTGTGGTGGTGTTCAGGTAATTGCGCTTCTCCGGCCGCTTGCTTCCTTCCTTTTCCACTGCTGACGAGGTACTAACGTCGTCGCC
 
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