CR00046

CRIMIC CR00046

Gene CG6903

CG6903

Information

Donor Location Cytology FBti Stock number
pM14X:4919756..49197564D6FBti020072781140

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK GGATCGCAATCGAGTTGGGCGTTA CAGCGACCAAAGGTTCTTGTTCATGGG

Images

Construct Design Sequences

sgRNA sequence GCCATCGCCTATAGCCCACTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TAGTCCAATCCAGACGCTCG GCCATCGCCTATAGCCCAC ATGGCCACTGTGCATCTTGT ATGCTTTACCCGCGAAAGGT
 
Amplicon 5' Amplicon 3'
TAGTCCAATCCAGACGCTCGTCGCGTTTAAATGCAAGTTTGTTTGTGTCTGTGTCGTAAGTAACTGTTGAATGCAATTTGGCTATTTGGCAATTGCAGTTGCAACTTTGCAAACTGCAACTTTGTGAATGAGAAGCGCGACAAGAACGCAAATCACCGAAAAATGTCGCTTTTTGTGGAATATACGGAGCCGAAGTGGCGCCACCTGGATATGCGCGCGCTATCCGTTGACGAGTTTTACCTGTACCTGCGCTCGGAGTTCAGCAGCCAGACCCATCTGTACTCACTGTCCGACGAATGCTACACGGTAATAAATTGATGGCCCCATGTAATCACATCGCGCAATCGGTTTCCCTTTCAATTGGGTGGCCATCAGTGAATTCGATTTTAATTGTATTCTTTGCCGTTTTCAATTCGCAGTGTCCGTATACCAAAGTGAAAGCTCTGCCCGCTGCCGACGTCGCTGCCGTCGTCGTTGAACCGTTACGTTTGAGTTCGGCATATTCGCTGAATTTCAAAATATTCGACCTCGATCAGGGTCACTATGCCTACGACAATGTGTGAGTATCACTCAACGTACAGTGGGCACTCGCTTAGAATGGCGCGGAGTCACAAGGTACAGTGGTCACACGCTGAGGTTGAACGCTAGCACTTCTTTGAGTAAATCTACAATACTTCTCTCAAGAATCTTGTAATGATTAGAATGGTTTATTGTGACCAAGTGGGCTATAGGCGATGGC ATGGCCACTGTGCATCTTGTGATTAGCATACATGTACATGTAACTAGTTTAAGCGCAACTGCTTGCACCTGTTCTCCTCTAAGTCTGTATGATCAGCAGTGTATCTTGAATCGATTCGATTTTCACGCAGCTCGTCGCTCTGCTGGCTGCGTCGCAGCGATCTGCAGGAATTCGGTGTCTACAACTTGACCCTGCAAGCGAATGGCACCTGTGCCTTTGCCGTGGACAAGCCGGGCGTACCAATTAATTACGGTGAGTGCCGCTTTAATCCGCCAAGTGCCACTCCAGATACATATTACTTATCGCTAATACTATGATCCCCGCAGCTTTCATCGCCGTTTTCGTTCTGGTTGCCGCCCTGCTAATCGCCCTGAAGCTAGTGAGCTGCGCCTGGCGTTATTATCGCTATGACGAGGCCACCGCCGCTGCCGACAGCATCGGCGAAGCCGCCACCAAGGCGACGCAGAGGAAGCGCCTCCGCAGCCTGGACACCTTTCGCGGGTAAAGCAT
 
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