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CR00048
CRIMIC CR00048
CG9565
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM14 | :.. | | | Order from Bellen Lab |
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
|
|
AGTAGCCATCATTGACTTTCACATGGGC |
TTGAGGAACGGCGCCAGTTTGGACA |
Images
Construct Design Sequences
| sgRNA sequence |
TGAATACCCTATCTGGTGACAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| ACACACTACCGCATACCGTG |
TCATGTCAGTTCGCAGAGGG |
AGGGTAACGATGGCTTTGAGAG |
AAAGTCGTGATGAGCAGCCA |
| Amplicon 5' |
Amplicon 3' |
| ACACACTACCGCATACCGTGCTCTCCCCATACACAAAGATTAACCAAAATGTGGCTTTCCGACTGCCGTTCGGTATGTCCGTGCTTCAGCTCCTGTATCCCATTCACTTTTAGTTGGGCACCTTAGTTAGACATGGAATGCTGACAAGTGGTTGAGAATGGGTTTTGTTCATGTTCATGTTTCAGACTTCGCGATTTCTTTCGAATGGATATCTAGATAGCGAGTAAAAATAGTCCAAAGATTACGAGGAACTCTTAGTACAAAATTATACATATATTTCTCTTAAGTTGTTGTGCATTACCTTTTTCTATTTCTATATTTCCATATAGTTTGGGTAATTTGCAAATTGTTCCTATGATGAATGCACATTTTCTGAAAACCCAAGGCATATCGTAAACTTGGAATGCTCTGCTGACTGCTGAAGGGTTAACACTTTTGAGTACATAGAGCACCATATGAAAATGGGCAGCAGTGGGGGATGTGAGAGAAATGTAAAGCACAAGGCGTTGAGTGAGCGCTAAAGAACGCACGGTATCTATACAATCCGAGCGGAAAAGCTTCGGTCTACTTATCAGATGCCCTAGACCAAAACATGTGATAACAGATAGCAGCAACATATTTAGATTATTAGATACACGTTGGTAGGCAATTTGATGGGTTTGCAACAATTCGATTCTCATTCCAGATGACGCGCTACAAGCAGACCGAATTCACGGAGGACGACTCGAGTTCCATCGGCGGCATTCAATTGAACGAAGCGACCGGCCATACGGGCATGCAGATACGCTACCACACCGCCCGGGTATGTAAGAAACCTGTCGGGGTAACACCGTGCATCCGTGCAATGCAATTCAGTCGGATAGCCACTTAGTTGTCGTTGGGCTTCAGCTTTATCTTTGTGGCAATGACCCAGAAACGAGTGGCAAATGGGGCCAAAGTGAGCGCTTTTCTCATTTGCTCGAAGGTTGCCAGTTGACAGTGAAAAGTCTGCTCTTATGAGTGGGAGCGCGTTGGTGTGAGCTTTTCCCTCTGCGAACTGACATGA |
AGGGTAACGATGGCTTTGAGAGGCGGTTCGTTGTTCAAATAAATTTAAACTTCATGTGATATTATTCCATAGATATACGAAAGCACTTGACCGTATACAACTGTATCAACAAAATGTCTAAAAGCAAGTTAAATTACTATAGAATTTAAACAGTGTGGTACAATATTTGTCTTTGGAGACTGCATTTCATGAGTCGTTGCGGCTTCTGAAAAGCCATTTATCTATATCTCAGCGATATATGGAAAAATTAGCAGGCAAGTGCCTTACGCTTACGAAATGTATCCCATGTAGATACCACCCCAATGTGTACAAGTTTGAACTCATTTGAACCGCCGTCGTCTTGTTTACAAAACACACAATTCGCTGGAAAAGATTTATGGGGCGAATCTTTTGTGCCGCCATGTGGAGGCCAGAATGATTGGTGGCTAATGCGGCGTTTTCATTTCACAGGCTACATGGAATTGGCGCTCGAGAAACAAGACGGAGAAATGGCTGCTCATCACGACTTT |
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