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CRIMIC CR00060

Gene CG15111

CG15111

Information

Donor	Location	Cytology	FBti	Stock number
pM14	2R:19139513..19139513	56B5	FBti0199465	78872

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGTTAATTGGCCACAAGTGCATGGC	GTACAGGGCGGAAAATTTGGAACGTGTCA

Images

Construct Design Sequences

sgRNA sequence	AGGCAAAGCGGATTAATATACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAAAGGTCGCTATCCGGAG	TGCAGGCAAAGCGGATTAA	TGCCTGCAAACCCAATTGTA	TTCAGATCGTATGGCTGGCG

 

Amplicon 5'

Amplicon 3'

TCAAAGGTCGCTATCCGGAGGAGGAGCTATCGGGTCCGGTTCGGTCTTGCATGCGTCCCATCTGATTAATCCGTCCAGCAAAATAAAAAACAAAAAAAAAAAAAACCAGAACAAATCAAAACAATCTGAATTAACCAACAAACTTATTTATCTCTCTTTTCGCCCGGGAAAGCTACGCACACAAAAACGCACAACATCAGTAACATTCAGTGTTAAGTCGCTTTCCCCGATAGAAAGTTATATCGCTAACTATATATTAAGACGATAAATACAGAACGGACGTAAAACATTCACAATGTACGAGATTCATAATTGCCTAAGGTCCGTTTTCTTAGCCACACTCATTCCCGGAACGATCCTACTTAGTTGGCTAACTGGTCTGTAAGTATCCACGCTCGATTCGATGTTGTGCGCTTGTTTTTTACTACTTTCGTAGACATAACCCGAAACCCATTCTTCTGATTTATAATCCGAACATCGTGACATCGACATTTCTCTTTCGCGGCTAATTTCGCCGGCTCTGTTCCGATTCCGCTTTTCCGTATATTAATCCGCTTTGCCTGCA

TGCCTGCAAACCCAATTGTATATACACATTATCCCCTGATCCCTGACGCTCACATCCGCACCAGCTTGCTTGTCCTTTATGTTCTTTTGTCACTTAACTCTGTTGTTAGTCCCATCCTCTTAGCCACCCAGTTACCGTTCACCTCTCACCAATTGACATTTCTCGCAGGGTGGGTCTGCGATGTCTGCAAGCTTGTCTGCTGATCTTCTTCCTGATCTTCGTGGTCCTACCGTTGATCTTTCGGTATTCCGTAACGTTCCAGCGTGGCATCCTATTTCTGACATTTAGTAAGTATCCAGATAACCGTGGAAGTTTCATTTGTTCAATATTAATCGAATTTCTCGTATTAGTTAAGTATCCCAAAGGACTTGATCTCACCAAGCCGGAAAGCGTGGGTCTTTATGCCACACGAAACTTCTACATAACCGTAAAGGATCACGATCAGGACGAGGATGGAGTGCGAGTTGGTGTGTGGCACGTGCTGCCCAGCAATGCAGTGCGTCGTTTTAAGCGCGAACTGCGCGTGGAGGAGGAAGTGGCCCAGGACCCGGATCAGCAGCTGGATCCTGCACCTGGCAACGAACGCGAGCTGAAGGAGCTATCGCCAGCCATACGATCTGAA

 

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