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CRIMIC CR00064

Gene Pfk

CG4001

Information

Donor	Location	Cytology	FBti	Stock number
pM14	2R:10111737..10111755	46F1	FBti0199466	78873

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCATTTGCCGCCATTTTCATGGTCCTA	TTGGGCGGCATTTCGGGAATGAATATGA

Images

Construct Design Sequences

sgRNA sequence	CCGATTGGAGATTATCTAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCGAATTTCCCCATTCCCA	CGGCCCAATAGTCGTGGTTT	TCTCCAATCGGTGCGAAAAGA	GACCACTCCTGACGGAACAG

 

Amplicon 5'

Amplicon 3'

CCCGAATTTCCCCATTCCCAGCGGTGAAAGCTCGCGAAAGCTTTTACTCGCCCAGTGATAACCAGCTGATCAACAGCTGAACGATTGTGGGTCGAACATAAACAAATTGGTTAAACGCTATTTTCCGCTCGTTGCTAGTCGCGAAAAGTCCGTTCATAACAGACAGAATCCTCAGATTTTCGACCTAAATCAAACCAAGTTAGGGCAATCGCGTTGGAGCGAACTCTTGGGCGGATTTAGCCATCCGAAGTTTGCATTGCATCAGTACGCACTTACAGAAAGCCCGTGGCTGTGTGCGTGTGTCCGTGTGTGTTGTGTATGCGTCTCTGGCCTGAAGGTCACCTTGAAACGTCAAAAGCAAAAGCGAAACTTGTGAATACCGCCTGCGAGCGTACATATTGCTAGTAACTGAAAAGCCACGGCAAGGAGCAGTAGTAGTATTTATAGCCCACTTGGATATAACCCAGCTCCAGCGGCGCTGCTCGCATAGTTGCAAAACAGGGAAACTCCCTACCGAGTACAATAGTGGTTCATAGTGTGTGCTTTGTGCGATATCCGTTACGACCCCAGTTTGATTTACATATAGTGCAAAAGATTGAAAAATGAATTCCGAGATTAATCAACGATTCCTGGCCCGCGGCTCGCAGAAGGACAAAGGACTGGCGGTCTTCACCAGCGGCGGGGATTCCCAGGGCATGAATGCCGCCGTGCGAGCCTGTGTCCGTATGGCCATCTATTTGGGCTGCAAGGTGAGTTGGTGACCTCTGTCAAGTACTTTCCGCCCACGCAGCATCGCGTCCGGTGACCCCGACCCCCAGGCACAGGCAACAAACCCATGTGAACCCAGAAATTTACGTAAAACACCTGGTAATTTGCTTATCAGCAGCCGTTAAACCACGACTATTGGGCCG

TCTCCAATCGGTGCGAAAAGAAATTCAAGAATATATGTGTACATGCACATTGACATTGGGTTTATTTTCGGGTCCCTCACATGCGATTTCGATTTCGGCTAATTTGTTTCGTTTTTTTGTTGTCGCTCTTGGTTTCTATTCGGTTTATTTAAATGAGTTCTTTAATTTGTTAGGGAACTTCTGGCCGGCCGTTGGTCCCTCGAAAATATGCAAAAAATGTGTCAATTGCTAGCCAATTTAAATTGATTAAAATCTATTCATTTGATTTCAATTTCTTGGGGGTCAATAAAAGTAAAGTTATTGCCACGCTTTGCATGACAGCGCTTGATATGTTCAATAAATTGAACAAAATTAATAAATATATCATGAGTATGCTAAACAAATGGTTCCAAATCAGTTCAAGTAATCTAACCTTTGTTTTTAATCTACTAGTGCCTTGGCATCATTATTGTTTATGTTATTTTTTCACTTTTCAAATTAAGAGCGTTATAAAATTTGAATTCTTGGTTATGTGAGTTGCTTAAAAAGTATCGATTTAACATCGATTACATTGTAATTACCTGTAACGGTATTTTGAATATGCAAATGTTTTGTTGTTTAAAACTTAAACTTATTCAGTAAAAGTTGAGAAAGTATATGAGATAAGTCATATCATATTAATTTCTATTGACTGTTCTTTACTGCTTCCTATTAAAATATTACGACTTTGGCAGAGTGAAAATGAAATTAAAATAATTTATACTCGAATTCGGTGATTCATTTTTAATTGTGCTAATGATGTCATGTGCCATGAAGATACCAAACTCTATACCAAACTGTTTTACACCATATCTACAATTGATTCGTGTTTACCTTCGGCAAGGTCTACTTCATTCGTGAAGGCTACCAGGGCATGGTGGATGGTGGCGACTGCATCCAGGAAGCCAACTGGGCCTCAGTCTCATCCATTATCCATCGTGGTGGCACCATCATTGGCTCCGCCCGTTGCCAGGACTTCCGTGAGCGTCAGGGTCGCTTGAAAGCCGCTAACAACCTGATTCAGCGAGGAATCACCAATCTGGTGGTCATTGGAGGCGATGGCTCCCTCACGGGCGCCAATCTGTTCCGTCAGGAGTGGTC

 

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