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CRIMIC CR00071

Gene pyd3

CG3027

Information

Donor	Location	Cytology	FBti	Stock number
pM36	3R:7749712..7749722	84D14	FBti0200729	81141

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	CGACACAGGTTCAGATCGAGTTCAAC	TCCCATCGATTCACTCCATTCGGGT

Images

Construct Design Sequences

sgRNA sequence	CTCGTAGTAAATAAGCATTACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCGCCGTAGTAGGGATTAC	TTACTACGAGTGGGTTACATTGT	ACGGGCAACTTAAGGTAGC	AAGGATATGCGCCGAACCC

 

Amplicon 5'

Amplicon 3'

GGCGCCGTAGTAGGGATTACGATAGAGTTTTGGATGGCGCCAACTCTGACTATCCGGCGCTTGCGAGTTTGCTCCTCTCGCGCCGTGAAGCGATAGCCCTTGATGTCGAATCCATTCTGTTCGGCAATATCTTTCGCACTTGTGGGCAGCTCAAGCGTTTGACTGCCAAGTAAAATTATATTATACCGCGATTTAATACAATACTTTGTGTATAAAGCTTAAATAATTATTTTTAATTTATTATCTTTATGGACAGATGTTCCTAATTATGGCTATATATTTATTGACCATTTTGACCCCAATAAAATTTTTGTGATCCTTACTGTGTGGTCAGAAGCTTATCTGTATTTCTGATAGTCGGAAAATATGGAGTGATTAGACTGACAACACAAAGTAAAGGAAAAATGGAATAACTAATTATGTTCTATAATTTTGTTCACATCACAATGGTTTTATAGTTTTCTAACACAGATAAAACCAATATACTGATAAAAATATATCCAATTTTTTATTTTTATTTTGCTTGCTTCGGCTTCGTGATCGTTTCACTTTCATCACCAGATGTTCTCAAGGGTATATATGTTAATGTGTAGATGCAAACGTAGCTCTGTCAAAGCAAGTTCTTTATTGCAATGTAATACAATGTAACCCACTCGTAGTAA

ACGGGCAACTTAAGGTAGCAATGAAATGCTTTTGTATGCTCCCCTTCTGTTTGCGAAAAGATTAAGATTAAAACATTTTTAGAATAATCTGGAGGTTTTATAGGCACCCACCCCCACATGATGTTCTTACTTGATTTTAATGGTCTTTTAACTGTACTCACTCTTCTTCTACGCCGTACAAAATTCTCTTAACCTCCTTTAGTTCATCGGGTGGTAAATGCTTTTCCAAGCAATCATTTAAATTTTTCAGTTCAAATGCTGACATTTTTCCAATTTTACAGCAACAAAATATCTCATCAATTATCGGACTCAATTGATAGAACAACCTTCTCCGACCACCGCAGTATATGAAATGAGTCTGCTGACGATCGGATTCTCATCTTATCTACACTTCTCTATTTTCGTCTCTCTCTCTCTTGGGTTTCTCTCGGCATCTCTCCCAAGTGTTATAAAAATAGTGAAAAACACATACATATTTAAGCACACTCGCTGGCATTTGAATAGCATACGAAATGAAATCTCACCGACAAAGCATATACTGAAAATTACGTGTGTAGTGTTGGGATTATTCTTAATTGTGTATATAAATAGAAATAAAGAAATAATTTTATATCATTTTCTTTTTTAATTTATGATCATTTTAAAATTACTTTCAAAATAACAGTTATCTGATAGTCGAGGGTGTCGACTATAGCAAGGTCTCTCGTTACATGTTCAATTTATTTATTTATTTTCTTGCAATATATACAGATAAACAACAACAGTAATAACATTGAAACCGGCATTTTATAATCCAGCAATCTACAAATATCACAAAAATAAACCTTCTGAGACCATACTTGACGATTTGAATTTCAAAGATCTGTCGTCAATTTAAACTTGAGAAAAGTTTTCTGTAAGTAATAAAAACTTATCATTTCTGGTGAGAAAAGAAATTTTATAAATTGTTTCTGCTGGCAAATTTAGTTTGATAGGTCACATTTCGGTCGATATTATTCAAATTTCATGCTAGGCTCTAAATCAAAAATATCTTAAGACCTCTCCTTAAAATGCAGATAAGTCCCGCGTGAGCTGATTTTGTCAAACTTAAGTAAATATTGAGCGGGTTCGGCGCATATCCTT

 

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