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CRIMIC CR00083

Gene Sec23

CG1250

Information

Donor	Location	Cytology	FBti	Stock number
pM36	3R:5652282..5652282	83B7	FBti0199467	78874

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCCTACTTCTTTGAGGTGGTCAACCA	ATTATGCAGAACTGAGTCTGAGCGATCTG

Images

Construct Design Sequences

sgRNA sequence	AAGGGATACAAGCACCTAGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAGACGCACAACAACATGT	GGTCCAACACCAAAAGCCA	GGACCCTTCTAGGTGCTTGT	TGTGATCAGCAGAGACGAGC

 

Amplicon 5'

Amplicon 3'

GCAGACGCACAACAACATGTACGCCTACGGGCAGGTGAGTCTCGCAGCCAGCGCCATCAACTGGCCACATTTGATTGATCTTTGCAGCGCTCCATTTGGCGTCATCGAAAATTTAATCTCAATCGAAATCTCAATCTCGTTTTATGTTGGTTTTGTTTCTCTCATTGTATAATCCGTACCCGTATCCGTAACCGCTCCTTTGTCAATCGCAGGCGCCCATCGGTCAAGTCACGGTAGTACATATTTGTTTAATTTTCAACTTTTAAACTGGTGGAAAGGTGGATATTTGAAATTTAAGGGGCGGGTAAAAAATCTTTGGAAATTGGATATGTCCTTTGCTCTTGATAGCTATAAGTCCAAAATAAGTGTTTTGGATTTTGCAATAAATCTAAGATGGCCAACCCCACACGGCTTAATCTAAGCAAAGATTTTGTCTGTTCCTATTTTTTCATATTGTTTTTAATAATTTCAAACATGATTTATATATAAGTGGCTTTTGGTGTTGGACC

GGACCCTTCTAGGTGCTTGTATCCCTTTTTCATTCATCCTCCCCTCCCCACTTGGCACGATCCCACCCACCCATCGCATGACTAATTTTCCGGACATTATCTCGACAGGATGGCGGGGCTCCAGTGCTCACGGATGACGTTTCGCTGCAGCTGTTCATGGAGCACCTCAAGAAGCTGGCTGTGTCCACCACCACATAAATCGACTCTGGCTCTAGTTTCAGGCGCTTGCTTCCCTGGCGAAGTCGAAGAAGGCGAACGACACCTGGAATCAATGGTCCAGGCAATGAAATTACGAATATACAACCACTTATTCCAATTGATCGTCTTATAACGCAATAAACTATTCAAATTATGTTCAACAATAAGTGTGTTAATCCGCGATGCTTTTATTCGTAAGCAGCTGAATTAGAATTTACGTTGAGCGGAATGAGTTTGTAATTATAAAAACAAGGCATTAACAAAATGAAATGAGCATCTCTTTCATTCTTCATAACAACTATTAATACAAGTAAATCCGACGGGTTCCTGAAACCTACAAGATTTGTAAAAATTATTTTTTTGTAATATAAGACAGCAAAAGATTTATTTATATTTTTATCATAAATATTTGATCAAATTTCCGGACGCATTTAACTTTTAGATACCAAGCAATTGGTTATATTTTTCTGTATTTCTCTAGCACCCGTGATCAAACATATGCATTTTTTGTTTAACTTGAATGAAATATCTTCGATAAGCTTTGGGCAAATTACTCAGGTTTAAAAAACTTTGCGACACTTTCATTTTCATTCATTAAATTGAAACTTCTAAATGGTTGGAAAACACAACTGGTATAATAAGAATAGCAACCACTGCTTTCAAATCATTAGAGTTTTCAGTAACCCGCCGGAATGCCTCTCGACTAACTGCATCCTAAACTATTTGGTACACACTGACTAAGAAGGTCGCTCTTTCCTCGTGATTGCCGCATCCAGCTAGCCTAGTTGAGAAACCGTATGGACATCTTCTGATCCACGTCGGTGCGGTCCAGAGCTTTGCAGAAGTCCTCGAACGAGATCTTGCCCTGGCAGCACAGGTCCGCCTCCAGGATCGTCCTCTCCGCAATGCTGACCAACTGGTCCTGGCTAATGTTCGCCCCGACCATCATGTGCAGGATGGAGAGCAGCTCGTCTCTGCTGATCACA

 

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