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CRIMIC CR00090

Gene CG3270

CG3270

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2R:6693737..6693737	42C5	FBti0199468	78875

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGACCATATCCTGTGCCGAGGTCCT	TGTATCGCATCTTCTCGCACGCCTA

Images

Construct Design Sequences

sgRNA sequence	TCCCTATACCTTAAAATTGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAATGAAGCGAATATGCTG	AAGGTGTTCCTACAAAAATTG	GGAACACCTTCAATTTTAAGGT	TCTTCACAGACTCAAAAGCA

 

Amplicon 5'

Amplicon 3'

GGAATGAAGCGAATATGCTGCCTTATGCTAAGATAATATTATACAGTTTCTATACCTAGGACAATAAACAATACTTTGTGGGTTCCCTTATCAGTTGAAATTCGAAATCGGTGAGTTGGTGTTAGTTGTTTGTCAAATACAAATTCAATACCCAGAAATTAAAATATAGCTGATAATGTGTGCTAAAGCATCTATTAAATGTGGACAGTAAGTACCAATGCAGAACAAACCGTTTATTTGATATTTTAGGTCAGAAAAATTAGATTGTTCTTATTCACAGCGATTGAACCAAATTTTAATTATTTATCAAGAACTACAAAATAAAAATTGTGTCGAATGTGATGTTTAAATCCTTTACTCATGGGATATGATTGAAAAAGGATGTTTCCAGCCCAAATTAGCAGATCAGTCCGCCGAAGGAACTTTTACTGTAATTCCGCATTCATTGCTGTGCACACAAAGCACTTAGGAAAAGCTGCACAAATCTAATCTGGCGGATTTCAATGGCGTTATCATCCCACGAATGTGCAGGTGCCGGTGCTGGTTAGTGTGAAAAATGCTGGGCCTGCGACGAGTTCTGCGATTGGGTGCACTGCGTCAGCTTCGCTCGTTAAGTGGCAAGCCGCAAGGCGATGTCCTTCCCGGGAGCTGCGGAGTCCTGATAATAGGTGGCGGTGGCATGGGCGCCTCCTCCGCTTTCTGGCTGAAGTCCCGGGCCCTGCAGCTGGGCCGCAAGCTCAATGTGCTGGTGGTGGAGCGCGATGCTGGTGTAAGTACAAGGTGGCCTAAAATACGACACAGAGTTATTCAAAAAGTATCAACAATATCTTACAATTTTTGTAGGAACACCTT

GGAACACCTTCAATTTTAAGGTATAGGGATCCAAAATTTACCCCAATAATGTTTGAACCTTTGGTTTCAAAACGGTTAAAAATAATTATGTAGTTGCTTTGCTCTGATCATATATCCAATATCCAATATCTACCAATTTAATACTTTTCCTATTGTATTAAAAATTATTATTTATTCGGTATCTAACTGAAACTTATTCGCTATTGAATAACAACAATTTTTATACAATTTTCCAGTACACCAGCGCATCTACAGTGCTTTCTGTAGGCGGAGTGCGGCAGCAGTTCTCTTTGGCCGAGAACATAGAGATGTCGCTCTTCGGATACAACTTCGTGGTTAACGGCAGGGAGCATCTGGGCGACGTGGATCTGTGCTACCAGCCGAACGGCTACCTGATCCTTGCCTCCGAGAAGGGAGCCCACATCCTAGCAAAGAACTCAAAGCTACAGAACGAGCTGGGGGCCCGAAATGAGCTGCTAGGCCCGGAGGCACTGCGTCAGCGGTTTCCGTGGTTGTCCACAGAGGGCGTGGAGCTGGGATGCCACGGGATTGACAAGGAGGGATGGTTTGATCCGTGGGCCCTGCTCATGGGCTACAAGAAGAAGGCCCGTGCATTGGGGGCGAATTTCGCGAACGGTTCGGTAGTGGGTTTTGAGTGGAACGATTCGGGCGGCCTTTCTGGAGCTGTGGTCGACGCCGGAGATGTGTTGCAGCGCACGGTGAAGTTTGACACGTGCGTTCTGGCAGCGGGAGCGTACTCGGGTCAGGTGGCGCGTCTGGCAGGGATTGGTGATAAGGAAGCAAAGGAGGCTTCCCTGAGTGTAGCCCTGCCGGTGGAGCCTCGCAAGCGATACGTCTATGTGGTTAGCACTCAGGGTAAGAATTGTCCTGGGCTAGCCACACCTCTGACCGTTGATCCCGATGGCACCTACTTCAGGCGGGACGGTCTTTGCGGCAACTTCCTGTGCGGCCGCAGTCCAAACGAAGACGAGGAGCCGGAGTGCGAAACGCTGGACGTGGACCACGGGTACTTTGAAACGGATGTCTGGCCCACACTGGCGAACCGAGTTCCTGCTTTTGAGTCTGTGAAGA

 

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