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CRIMIC CR00108

Gene Shmt

CG3011

Information

Donor	Location	Cytology	FBti	Stock number
pM36	X:5914463..5914463	5C7	FBti0200730	81142

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	ATTCGGAGATTCAGGGCAAGGAGCA	GGGGACTCGCATTTCGATTGCCTTT

Images

Construct Design Sequences

sgRNA sequence	AGATCACCTTATTGGCTCTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGGCACAGTGTTCTTGTTG	GGCATGTGAATCCCTGGTGA	TGCCATAGAGCCAATAAGGTGAT	CGACTTGAACTGGTGACCCA

 

Amplicon 5'

Amplicon 3'

CGGGCACAGTGTTCTTGTTGCACGCGATGCCCACCTCCTCGAGGATGTACTCGGCCTTGGCGCCGGTCAGGCCAGCCTTACGCACATCGACCAGCACCAAATGGACGTCGGTGCCGCCGGTGGCCACCTGATAGCCTCGCGAAATGAGGCCATCGCACAGGGCCTTGGCATTCTTGAGCACCTGCGTCTGGTAGGCCTTGAATTCGGGACTCTTGGCCTGCTTGAAGGCGGTGGCAATGCCAGCCACGGCGTTGTTGTGCGGACCACCCTGGAGTGATGGAAACACCGCCTGGTTGATGCGCTCCTCCAGATCGTAGAGTACCTTGTCTCCATTGGCCTTGGTGCTGCGCACGCCCTTGCGGAAGAAGATCACGCCGGCGCGCGGACCTCGCAGTGTCTTGTGCGTGGTGGTGGTCACAATGTCGGCCCATTCGAACGGCGATGGTATCAATCCCGCGGCCACAATGCCCGCCACATGGGCCATGTCGGCCATCAGGTAGGCGCCCACATCATCGCAAATCTGTCGGAAACGCGCATAGTCCAGCAGACGGGAGTAGCACGATATGCCAGCAATGATGATCTGCGGCCGGAAATTCTTCGCCGCCTCCGCCAACTTATCGTAATCGATGATGCCCGTCTCCGGGTTCACTTTGTACGGCATGCTCTCGAAGAAGATCGATGTGGCCGATATCTTCTTGGTGGGCGTGAAGAAACCGTGCGTCAAGTGACCGCCATCGGGCAGATCCAGGCCCATGATGCGATCGTGGGGCCGGCAGACGCCCGTGTAGACAGCCAGATTGGCCGGGGATCCGGAATAAGGCTGCACATTAACGCCCCACTTCTCATCGTCCAGGTTGAACAGCTCGCGTCCGCGTTGCTGGGCGAGCAGCTCTATGCGGTCGATGTACTCGTTGCCACCGTAGTACCTGCAAGTCAAAGCGATGACCAGGATTAGAGCAGGATCACCAGGGATTCACATGCC

TGCCATAGAGCCAATAAGGTGATCTCTACCAGTAAGTATGATATCAGAACTAGTATAGAATCATTTAAAGATCCGACTATGATGTCTTCTTGCAGATCCGTGAGGAATCTTATATGTGCACCCACCTCTTGCCGGGATATCCCTCGGAGTACTTGTTGGTCAGGCAGGAGCTCAGGCTCTCGAGAACCGCCACCGAGGTGAAGTTCTCACTGGCGATCATCTCGAGTCCTTCGCGCTGGCGCTCCTTCTCCTTCTTGATCAGCTCGGCCAGCTCCGGATCGCCCTGTGCCAGCGGGGTTTGCAGCAGTTTCTGATCGGCCATATTCTGTTCGGATTTATATAGTATTGTATATTATATATATATATATATAGTAATTGGAATGCTGTGCAATGTGCGATGCGCTCGCCAAGACGATCGTTGAATATGTGCGGACCGTGGAAAATGAACGGCTGCCTGGTCAGCTTTATGCCATGTTTTTAATAACGCTTCGAAACAAAAATAATATTTATGTTCCCGCCGCCGATGGTTGCGAAAAGTTACAAACAAATACGGGTTCAATGGGTTCAAATAGCATTGGAAAAGTTCCGGCGATAAGGCGCAGCTATCGCCAAGGCGAAGATTACGCCTGGATGCACCATGGCAACCACTCGAGAGGCGCTCCCCCGATTCCCTATTCCCGATCCACGATTAGATTGGCCAAATACCAGGGAAACCAAGTGACTTGGGTGCAATAACACGCCCATGATCGAAAATGCGACCCCCCATGGATGGGAGAGCGCGTCTATTTGCGATCCGCTTATCGTCAAGTGATTATCATCCGTCTGGGTCACCAGTTCAAGTCG

 

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