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CRIMIC CR00122

Gene CG1358

CG1358

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2R:7604438..7604440	43E4	FBti0199698	79228

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACTGGCCAATGAAGCTGACGTAGAA	AACTAAACGGCACCACCGATCAGTC

Images

Construct Design Sequences

sgRNA sequence	AGTGATAGCCATTTAATACGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTCGGGCGTAGACTTTGTA	AGCCATTTAATACGAGGTGCA	TCACTATGGAATCGCGTGCA	ACACAGCACTCGCCTAGTTG

 

Amplicon 5'

Amplicon 3'

CGTCGGGCGTAGACTTTGTAGCCTAAGAAATAGAAAGTGGAAATTGAATGCCAGCCAGGTGAAAGCCACTTCAGAGTGCTCATGACGCGGCCCAAGGCTCCCCATTCCATCGCATCGCATCCGACCATCCTGCGGAGGAGGACCCCGTGTAGAGCTGCACCGGATTAACTGGATCAGGGGCCGGGATTACATATACTACTCACTACTTCTTCACTACTTCCACTCGCGCCTAAGGCCTTTTCCGAAGCTAATATCCATGCAAATGATGGCGCCCACCACCATCGCCCCATTATCGTAACAAGTTAATCCGCCCTTTCTGTTTGCCTAGTGACGTCGCGGCCTTGAGGGTTTTTCGGTACTTCTCTAAATTTCCAACTTAAAGGCCTCCAATGGCAATCTTGTTAGTCATACTAGCCAGCTTATGTCTCTAAATTTATATGGTATTATCATTATCCATAATAACGTTTGAAGTTATACAAAATTTCAATTTATTGAGTAATTTAAATAATTTTGTTTTGCTGCAATTATTTTGTTTTGAATATATATTTAATGTGTGATTCGTGAGCAAAATATCCTGAATAAGATGAAATTAGGCCCCATGAGGTTATCCTTTGGAAAAGGTTTCCTCGAAATTTGAAATTATTGGTCGACTTGTTTAAATAGCTTAATTATTTTCGATTCATGGACACCGTGATTTTCGCTAACGAGGTTCATTTAAGCAACTATTTTTGCTTTGACCTAAATCGAATTTGAATAAATTTCTGTTCAAATGGAGAGGAAATCCTTGGCTTTAAAGGCATTTTTGATTCGGCCATTTGTGGGAACGTTCAATTGTCCCAGGAGATCCTTATGATTAAAATGTTTGATTACAAATAGAACGGGTGTGATCTAAATGCACAGCTATTGAAATGCATTTAGTTTGCACCTCGTATTAAATGGCT

TCACTATGGAATCGCGTGCATCGGATTGCTTATATATGTATGTAGTATATATGTAGGCTTTGGTTCTATTTTTAACCATCTTTCTCGTTCCAATTTGTGACAATTCACAGGCGACGTCACTAACTTTTCGGCTTGCATGGCATTGAAAGTACAAAACGCTTTGATGCCCTGCTTTATTTGTATTTTTCAGTTTTCAGTTAAAGCACGTGTATGCACAAACACAGCCAGGTACAATGGCCACACATATGTTTACAGTGCACAGGAAGAAAAATAGGGTATTACTATTAAAAAAATAATTATTTAAGATGGCCCAGTGATGTAATTAAAATATATAGATGAACTTCTTCAGGTTCACAGAAGAGTTACAAAGTTCGATTTTGTATAAAATACATTTGCTTATGTAGATCTTGTATTTTTAGTTCATAGTTAATCAAAGGTGAATCTTTTTTCTTGCAGTGCTTGCGGCTGGCCTGCTTTTAAGTGAATGTGCGGCTGTGGCTTTGGTTTTGGCAGGCTCGCCGAGTGCATTTGTGTGTGGTGGTTGTGTGGTGCCTGGATTGCATACTGCACCCGAATGCCAGCTCTTCGCACACCCACACTCTTACACACCCGCACCACGCACCCATACATCTATGCAGCCGCAACACAATCACAGACACGATACTATTGCCTACCTGGAGGCGCAGTTAGGCCAAACCGCTCGTTCGCGTCCGACATGCAAAGTGCAGGTGCACATTCAGCTGCAGCGGCAAGGCAACTGCGCGACTGTTCGGCTTGAAATGCCAACTAGGCGAGTGCTGTGT

 

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