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CRIMIC CR00131

Gene Adk2

CG3140

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2R:24024779..24024787	60B4	FBti0199469	78876

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAATCCGCCAGAAGCCGAAGAGTT	AAGTCGCTCTGCGAGAGATTCGCA

Images

Construct Design Sequences

sgRNA sequence	TCTTGAGGCCAGGAATGTTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTATCCAGGCCTGAACTCC	GAGGCCAGGAATGTTCTGGT	GCATCTTATCTAGCCGATTGGT	TCATCGCTGCGACGGATTAG

 

Amplicon 5'

Amplicon 3'

GCTATCCAGGCCTGAACTCCGGCGTGGTCCTGCTGCTCCTAAACCGCATCCGTAACTCAAAGTCCTACCTGGAGAAGCTGACCCACTCGGAGGTGCACACCCTGGTCGCCAAGTACTCCTTCAAGGGCCACCTCGGCGACCAGGACTTCTTCACCCTGCTGGGATACGAGTACCCGAACTTAATCTACAGACTGGACTGCATTTGGAACCGCCAGCTGTGCACCTGGTGGAAGGATCACGGATACTCACAGATATTCGACGCTTATTTCCGGTGCGAGGGCAATATTAAAATGTACCATGGCAACTGTAATACGCGAATTCCAGAATAAATAAAATTGTTATAGGAATATTAAATTAATTAAATGCAGGGTATTTTTATTTGGTATAACTATTGTAATGGAACGTTATGTAATATACGGTGAATTTAATCTTAGTTTATAGACCATTATAAATGGTGATATATAAAAATGAAATTTAAAGATAAAAGACAGCCCACACCTATCACCATTTAAAATATCAAAACGATGTAAGAATGTTATCGATAGGCGAGAAGCAACAGAGTGGGAATATGCCGCACAGGGTGTTTCGTGTGAATGATTTCGCCTCTTGTTTCTTATCTTTAACCTTCATTTCTGGCTCCGTTTGGCTATGAATTAGGAGCAGTCCCGAATAATCAGTCGAGCATACGTAGTTAACGATGGCGCCGAATGCAGCAGTACCAGTGGAGCGATATGAGCCTGAGAACATCGGGATCAACGCCATTCTCCTGGGGCCGCCGGGTTCCGGCAAAGGAACGCAGGTGGGTCAAAATTACATAATGATGTGTTTTTTGTGACACGGACGCCATGTAGTCTATAAATAAATACAACGTGTTCAAGTGCGCGGTTTTTGTTTGCAAATAATCGGTGAAGCTCGTTAATTCTCTTGGAAGAGAATTAAAACTGGTGAAGTTATATAAGACGATGTGTACATGGAAATGTATATGTATGTATCTACGTGCCAAGTATGTACATATGCGTCAAGCAGTGTCTTTCTCTTGACCCTGAATCAAATCAAAGCCTTGGCGAGATGTGGCGAATTAAGTGGAACACCTTTAGGCAATCGATTGGGTATTATGCAGACCATCTATTAAAAGGCTACCAGAACATTCCTGGCCTC

GCATCTTATCTAGCCGATTGGTTACCTTGCTTACCTTCCGATATGGTGCTAAATTGATTAATTGATTAGCTCTAACTGAAACTTATCAATGTTTTTAATTATGCCCATTTCAGGCTCCCCTGCTGAAGGAGAAGTTCTGCGTGTGCCACCTGTCGACCGGCGACATGCTGCGGGCGGAGATTTCATCCGGTTCGAAGCTGGGAGCCGAGTTGAAGAAGGTCATGGATGCCGGCAAACTGGTCTCCGACGACCTGGTCGTGGACATGATCGACTCCAACCTGGACAAGCCCGAGTGCAAGAATGGATTCCTCCTGGACGGATTCCCCAGGACTGTCGTTCAAGCTGAGAAGGTAGCCACACGTGTGAAGCACTTTGGACTTTAGGTTATAATCCGTTCGAAACGCACTTTGCAGCTCGACACATTGCTGGACAAGAGGAAAACCAACTTGGATGCTGTCATCGAGTTTGCCATCGACGACAGTCTGTTGGTGAGGCGCATCACTGGCCGCTTGATCCACCAGGCCAGCGGGCGTTCGTATCACGAAGAGTTTGCCCCTCCAAAGAAGCCAATGACGGACGATGTAAGTATCTCTATAGGACGCTATCCAAGAACTCTAAATGAAAGCAGACCTTGTGTACTTGCAGGTCACTGGAGAGCCCCTAATCCGTCGCAGCGATGA

 

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