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CRIMIC CR00132

Gene AkhR

CG11325

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2L:6713987..6713987	27A1	FBti0199470	78877

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCATCACCTGGCCTCTTCCATT	TAGTACACAGCAAAACACGGTGTGGC

Images

Construct Design Sequences

sgRNA sequence	CACTTCGTTGACCCACTTAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGAGACAAGAGGAGCCAGT	TGCACTTCGTTGACCCACTT	CGAAGTGCAGTTGGAAAGGTA	CTCGACTGAGCCGCCAATAA

 

Amplicon 5'

Amplicon 3'

TGGAGACAAGAGGAGCCAGTGGTGGTGAGAGGCCGTCGGCCAACGATTGACACTTCGAGCCACCTTATCTGGCTATCAATTTGGTCCATTAGCGACAGTACTCACCTGTCGAGCGATATGCAGACCATCACGTAGCTGGACAGATACAGGCCAAAGACGCGGAAAAAGCTCATCAGGCGGCACATCAGGTCCGTGGATAGCCACTGCACCGTCCAGGCCCACACGATCTCCATTGGCATCAGGAGCAGCGTCACCATTAGATCGGCGATGGCCAGATGCATTAGCATGATATCAATACGCAAGGGACCGCGCAGTCGCCGCTTGGTCAGCAGATACAGCACGGTGCTGTTGCCAATTGTTGAGATCACAAACAGTATGCTGATCCAAATGAAAGACCACCACATTAGGTACCGGGAACACGGCGGTGCAGGAGGAGAAGGTTCAGAAGCGATTAAATTGGTGAGTGGGAAATTAAAGAAGTTTTTTCCCGAAGGTGGGTGTCGGCGTGGGTGCCATTAAGTGGGTCAACGAAGTGCA

CGAAGTGCAGTTGGAAAGGTAAACTTGTTGAATGAATGAGGTAAATGAATCGTACTCAATTGGTATCTATTGCAGACAATCTGGGCAGACAAAATTGATGTCCCTTCGAAGTAGCCAAATAGGGTGAATTTGAATAAACAGTTTCAGAACCGGTCAATTATATTGTTAATTTCAGGCACCTTTGGCTGCTGTGCTAAATTAAGCACTTTCTACGCCTTGACTTACTGAGCGACTCACACTTAATCGATTAAACAAATCTCTGGCATTCTATAAATTGAACCCTGCAATGATCCGCAAAGTTCAAATTCCAAGTGAGAATGATTAATCTCAGACAGATAGCCAGACACCCGAGGAGAAACCAGCGATGATGACCCCAGTATGATTTATAGGTGTTTATAATTCAGGCAGCGACACCCACATCGCCATATGAATTGATTAGTTTAGACTTGTTAAAGATAGTAAAAGTCAAAGTTGATAAGCTGAATATACACCAATGAGAGACGTGGGCGGTGATCTTGACGACCAGGTTGTAAAATGCTGGTCGGAATTCCACTCACCTGTACACGGTGATGGACAATCGGTGGCCATCATTGAAGACCATATCCTTGGTCAGGTGAATGGTGCCATTGGTGTCGTTCACATTCGACCAATTACTTAGATCACGATGATCATTCTCCTCAGCTACTTTTGCCATTTTTTGGGACAAGCTAATAAATTGTATCTGAATGCAACTGCATCAAGGATTTGCCTCAAAGATAACGTCTTTTATTCTAAATCAAAAGATACAGATACAGATGGATGTTAAGATACTATTTCTTGTTTTCGTTTGAATGTTTCATAATTGATTTTTGTATGCATGTTAATTATTGAAGTTTAAACTGTGTGCTTTATCATTTGGGTTCGTTTTGGGGGCCCGTCATTAAATTATTGTGAAACGTGTGCGCTGCTTTTTGACTTTTCGAATGCTTTATTATTGGCGGCTCAGTCGAG

 

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