CR00143

CRIMIC CR00143

Gene Gmap

CG33206

Information

Donor Location Cytology FBti Stock number
pM36X:15506755..1550678213C7FBti020073883162

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK CTGACCAAGGAGCAAGAATCTGGCTT ACACACTGGCATACTCTCTCTCTCTC

Images

Construct Design Sequences

sgRNA sequence TTGCATAAGAGTCCTTTGCCTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATCTGCTGACGGAACAGACC GAGCACCCAGTGACGATATC GCTGGCACCAGTTTGGACTA TCGCAATCCTTGATTGGCCT
 
Amplicon 5' Amplicon 3'
ATCTGCTGACGGAACAGACCAACCAGCGACTCCTGCAGCAACAGCAGGCGGAGTCCCAGGAGCAGCAGGCCAGCACTTTGCGGGATCTGGAACGTTTGCGGGCCCATCTCCTTGAGATCGAGGAGCTGCACACGCAGGAAACTGTCGAGCTGCAGCGCGATCTGGAGGAGTCGCGCTCCCGTCAAGCGATCCTCGAGCAGCAGGTTTCCAAATCCAGTACGGCGTACACATCGGCCAGGTAGGAAAATAGAACAGAACAATTTACAGCTGAGGGAGGGGTCACCTTGGTTGCCATTGGCTGATATATTCGTCACACCAGCGATAAGCGATCGAAGCTGGCTAATTGCATTCGCCACATGTTCCCACACGATTCCAATTCCAATGACAATTGCCATTCCACCCACTCCATTCCGCCGCCGGCGGAATCGGACCATGCAAATTGCCGTCCATATTTCCGCATGCCTGGCAGTTCGTCTTATCGCCAGTTGGCAAATCAATTTCGACCTGGTTTGGATATCGTCACTGGGTGCTC GCTGGCACCAGTTTGGACTAACTGCAGTTGCTATTTTTGGTTGGAAAAGGGGGGACTCTGTATCAAGTATCAATTAATTAATTTCGAAGCATTAGATTTTAAAAACTAGATTACTTTATTGAGAACTTTCTGCTGGGTGTTATTAAAATAATTACCTAAATTCAATAAAAAAAAATATAATGGAATTGGGCTTGGGAAAAGTGTTGATTGCTAGATTGTAGATGTGAAAGTAAGCCATGCACTTAAATGCATTTATATAGTTATTAAGTTACTTTTGCAAAAATGCAAAAACCTAGAGAACCAAGGAAATTTGAAATTACAATGAGCATTTCTTCGAAGTTTCATCGTCATTGAGTTAATCTTTTAATTATCCCCCTCAGCATTCGTGCGAATCAGCAGGCGGAGACGCTCCAGGCGCAGCACGCTCTGCTCCAGCAGCAGCGTGATGAGCTATTGGCCAAACTGGGCCAGTACGAGGATCGGGAGCTGAAGCAGCAGGCGGCGCTGACCAATCTGCAGTGTGCCCTGGAGCAGTTCCAAAATGGTAAGAGGACGCACACTATTTATAGCACATGACGATGATGACTAATACGGGTTGTCGTTTTTAGACAAGGATCATGACATCGAAATGGCCACGCAGAGGATTCGCCGCGAGATGCAGGCCCAGTTGGACCGACAGGGTCAGCTCCAGCTGGAGATGAGCGGCCTGCAGCAGCAGCTGGCGGAGGCCAATCAAGGATTGCGA
 
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