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CRIMIC CR00158

Gene CG30344

CG30344

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2R:9146468..9146476	45A13	FBti0199471	78878

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATAGGGAACGAGAGCGAGAACGTGA	CGGCGCTAATAAAGTTGAAAGCTCCG

Images

Construct Design Sequences

sgRNA sequence	CGCCAGTCGAGGCTTATCAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGATCGGACGCGGATAAAGT	GCCTCGACTGGCGTTCTAAT	CGGCCGGAATAGAAGTAAGC	GCACAGAGGGAGGCTTCATT

 

Amplicon 5'

Amplicon 3'

GGATCGGACGCGGATAAAGTAAGTATTTAATTTGACCCTTATAGTATACACATCATGTACAATTTGATATCTGTACTTTGTGTAGTTTCTGTTCTCGTTGCCTGGTTTTTATCTCTGTGCAAATTATTTTTGCTGCTTTGCTGGGCGTTTCGTGTTGTGATTTTATGGCGATGAGGAAAATGGCAATTTTAAACAAATTTATCCAGTAACTGTAAACAAAAACTGAAGTTTAAGTTACAAAAACTGCTGAACAATTTTAAAACCACAATTAATGTATCACAATATTAACTTGTTTTACGAGTAATTGCTACTATTATGACCTTTGCTGTATAGAACAATGTATTTCGCATTTGGCTCGCCGATATTCTTCCAAGACGTGACCTACTTAGTATATCTTACTATATCAGCATCCGACGTGACAAAGTAAAATCAAATTAGCAAATCGTCTCAACCTAATGCAAAACTGCCATTGTTTGGAGGCTAGAGATAAGGTAATTAGAACGCCAGTCGAGGC

CGGCCGGAATAGAAGTAAGCAAAATCCTTTGTTACTGCATTTCGAAGCAGTATCGAAGTATTTACTAGGAAAGCGTTAAAATTATAATTATACGTACGAGATATGGCACATTGACTCATCCACAGTTGTACAGTTGTGCAGTTTACTAAAGTGAACTTCTGTTTTTTCAGGAAGTCGAGGTGATAGTACAGACGTATTCTGCAAATATTATGATGACAACCTCTCTGCTAGAGAGCATTATTCCTGCCTTCGCGAGTCTGTTCCTGGGTCCCTGGTCCGACAAGTTCGGAAGGCGGCCAATCCTGCTGACAACTTTTACGGGTATATAATCATCAGTCAGGACATCTTAATTGCATCTTGTACAAATGACTCTTATTGTATTTAAAGGCTACCTCACTGGCGCACTCATACTGATAGTAATCACTTACATAACGAGGTCCACGAATATAAGTCCATGGTGGTTCCTCCTTTCCTCGGTTCCTTCCGTCGTAAGCGGCGGAACTTGCGCCTTGATTACTGGAATCTATTGCTACATATCCGATGTGGCCAAGGAGCGAAAGAAGGCTCTGAGGTGAGTATGGGTAGTCTTGATTTATTAATCCAAAGAATGCCATTTCATTTTGATATATTATTTGAAAGAATGGTCCTCAATGAAGCCTCCCTCTGTGC

 

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