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CRIMIC CR00184

Gene Aldh

CG3752

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2L:9389244..9389257	30B1	FBti0199472	78879

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCACTTATCAGTGTTGGCAATGAGCT	GGCAACCAACTTAGCGCCCTGTTT

Images

Construct Design Sequences

sgRNA sequence	CAGTTTCCACTTCTTATCACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTCCGGTGTAGGTCTTCCC	GGAAACTGCCTATGCCAACA	CGGCAGCCCTATTATTTTGGG	GACCTGATCGCGTTCCATGA

 

Amplicon 5'

Amplicon 3'

TCTCCGGTGTAGGTCTTCCCCATTTCGATTTTAACGAAAAGAAATTGGCAAATCAGCGCAACGCAAGCGAGTCTCACCGCATTCAGATCACGTACACCAAATGTTTATCGGCAGATGTAGGGGATTGTAGGGGATTAGGCTGCTTAAGCGAATCGAAAGACCGGCGACTAAAATAAATATACAAAGGCGATATCGAAAAAGCAAAAACAACAATCGCATATGTAGTTCGCCGGCTATTGTTGTTGTTATTTCTGCTTCAGCTTCTCATTGTTTGCCATACCCGGTTTCTTTGTTACGTCAGCAGTGGCGCTCGCACACGCAAAACAAAAATTTGTTTGCCGCAAATTCTCATCATTTATTCTTCTCATCGCCTCGCGCTTTCATTTCTTCTCTAGGGAAATTTGCATAAATTTAACCATTGTCATTTTTTATCTTTAGTGTCACCAATTAAAACTAGCGTTCGTTCGATTTAATTGAAAAGGAATTGCGGTTAGGAAGCGAGCCAAATGTGGTTTTTGTCCAGATGTTAAATGGGATCCAATGAAATTAGAGCAGTTCGTATATAGTAAACATTTTTGCAGAAAAAACTTGAAAATCCAATGTGCACGGTTTTTATTTGTCTATATATATTTCAGAATTTTTAAAGAAACTCCTAGCTTACATCGCTCTTAAACTGTCTTTAAATAATTGTTAGTGTTCTATGTTTTCCAAAAACAAGGAAGTTCTTAAAAATTTGTATATAATAAATATTTTTGCAAATGAACTTGATTTTCAAATATGCATGTCTTGCATACCAATATATTTTTGTTTTTAAGTCTAAAAATGAAATCTTGCATACCTCTTAAATTGGCTTAGAATAGCAAATAACATATATATAGACCATATATGTACATATATGCTGAACCCTAAAGCAAAATATTTCAATTTTACAAGAATTGAGCATTCGTCTATTTTGAGTGAACTGCATATTATGCAACCTTTATAGTACTTATACATATGCCATAATCTGAGAATCTGCTTTCATAACAAGAAATATCAGCCCATAATGTCTCGCGCACCACAAACGTTCTAATCTCTTGATTTATCTTATTGTATTTGTGTTTGTTGGCATAGGCAGTTTCC

CGGCAGCCCTATTATTTTGGGGGTCTTTACTGGGCGAGATAACATGGCACACTTATAAGATTGGCTTCCCTAATCGAACCAGTATTAGACTCGTCTAGCCAGACCCCCGAAAACTAATGCACACGTGCGTCGTCGCTATGTTTATATTTTGCTATGTCTGGCGGAAAGTTTTATATTCGCAAGCACATTGTGAAATTCAAAAGTAGATGTATGCAAAAATAAAAACAAAAAGAAATAAAAATGTTCTATAAAATAGGATGACGTGAGGGGTTGGACGCGAGGGCTCACTTCGTTTCAATTTTAATCAGTTAGCCGGTAAACGTTTTAGATTACCCAGACTGGGGGATTGTCAGATTCAGGTAGCCATGCGAACGAAACAATCAGTCAATTGCGATAGAGGAATAACACGCACGGCAGGCAATAATAACAAATAACAGCAGTCAAGGGCTTATCATATAGAGGAGTGGCTTGTTTGTCAATCCAACAAAACGATGTAAAATACAAATGAATTCAATAGAAGAGATTGTGAATATGATATGGCACTAATTGAAGCTGTTAACTACTTATTGCAGGTATTTATCAACAACGAGTGGCACAAGAGCAAGTCGGGCAAAATATTTGAAACCATCAACCCGACCACAGCGGAAGTCATTGCCGAAATTCAGTGTGCTGATAAGGAAGATATCGATATTGCCGTCCAGGCTGCACGCAATGCATTCAAGTGAGTGTGCAATTTGGATATAAATCGACTTTGCTTATTAATAACAACTCTATGATCACTAGGTTGGGATCTCCATGGCGTCGCATGGATGCCTCGGAGCGGGGTCGCCTGCTCTACCGCCTGGCTGATCTCATGGAACGCGATCAGGTC

 

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