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CRIMIC CR00189

Gene Ass

CG1315

Information

Donor	Location	Cytology	FBti	Stock number
pM36	3R:6643781..6643792	84A1	FBti0199473	78880

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAATGTGTGTTCTATCGCCTTGGTTTG	GAGGGTCACACGTTCATACGGACAAT

Images

Construct Design Sequences

sgRNA sequence	AGAGAGGCAATCCAACCCGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAGTACTTCGCGGTCCAAA	TATCGCACGCCAAGTCAACA	AAGAGAGGCAATCCAACCCG	ACAAAGGGAACGAGGCGTAG

 

Amplicon 5'

Amplicon 3'

GGAGTACTTCGCGGTCCAAAGCAAACACCTCGAGGTCCTGGTGGGCAGCAAAGAGGATGGTGCCTCCAGGCGTCTCGTACACCCCCCGGGACTTCAGTCCCACAAAGCGATTTTCCACGATGTCAATCCGCCCAATGCCATATGATCCGCCAAGCTTATTGAGGAAATCTAGCATCTCCAGGGGTTTAGTGTAGACCCGACCGCCGGGCAAATCCTCCACACTGGAGGGCAGTCCCCGATCAAATTGAATGACCAAGTGAACCGGATCCCGTGGAGCCCTTGTCAGGGGATCGACTGTCATCTCGTAGAGATTTTCCGGAGCCACTGTGTTGGGATCTTCGAGTATTCCGGACTCGTAGCTGATGTGCAGGATGTTGGCATCGGTGCTCCAGGGTGTGGCTGGCTTGGCGCTTACCTCGATTCCGTGTTGCTGGGCATAGGCAATGAGGTCCTGTCGACCTTGAAACTGGCAGCAGAATTCTACATCCCTCCAGGGAGCGATGATCTGGAAAAAAGTCGATTTCGCGTTTATAGCACAGTCAAATCTTTAAATTTGATCACATATGTATATTAAATTTCTATTTTCTATTTGTCGGTTGAAATGGGGCTTTTTGACGTTACTAACACTCAAAAGAACATTAATTTAGTACCAGTTCATATAAATCGATCATTGTGATGAAATATTCTGCCTGCTAAGCAACAAAAACCATGGCAAACCAAAAAGCTCTTGACTTCTTTATTTGAAATGAAGAAAAACTAAAAAATAGTGCAATCTTTGTGATTTCAAAGTAACAAGCAAAACGGATTTAAGCCGTAACTTGAAATGGATTTGATCAGCATGTCGACGAACCGGTTCTTAAACCGGAACCTACTCGATCGTTACGGTTTAAACCACAAACCAAAAGCTAAAATTTTATGCGTTCACGGCACTTCTTGTAGCATTGATTTGTTTATAGATTACACTCAAAGATGTTAATGTAGCTAGTCAGACAACTTGGGTACGCACAACTTTGTTGTTGACTTGGCGTGCGATA

AAGAGAGGCAATCCAACCCGACGGGTCTTACCACTCAATTAGTACGGATACCTCTAAAAATTTTGTACGTACCTTCAGATCCGGTTTCAGGGCGTAGGCGCACAACTCGAAGCGCACCTGGTCGTTTCCCTTTCCGGTGGCTCCGTGGGCCAGATACTTGGCTCCATACTCGCGGGCGACCTCCATCAGGGCTACACTGATGCAGGGCCTGGCCAGAGAAGTGCCCAGTAGATAGCGCTCCTCGTACACCAGACCCATTTGTACGGCCGGCCAGATATAGTCCTCGACGAAGGACTGCTTGACATCGGCCACGATAACCTGAGTCGATAGGACGAAACAAGTATATATTCGAAAATTAATTAATTCCGAAATTTCAATTTCATCCGACATGTATCTACATATGCCACACTTCTGGTTGGACAACCTTTTTTGCGCCAATCTTAAGAGCCTTCTTCTCGGCGGCGGTGAAATCTTCCTTCTGGCCTACATCGGCCAGGACGCAAATGACCTCATACTGCTTGTCCAGCAACCACTTGAGGACGCAACTAGTGTCCAGGCCACCGGAGTAAGCCAAAATTACGGTTTCCTTAGGCATATTTCTCGGATGCCCGTCCACTTGTCACGGTTGTGCAAAAAATACTACATTTCTATGATTATTAGCTCGTTGAGCTTGGAGTAATCTCCGTCTAATCAAACCTGCTTATCAATCCCTACGCCTCGTTCCCTTTGT

 

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