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CRIMIC CR00195

Gene mEFTs

CG6412

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2L:17499943..17499970	36C10	FBti0199474	78881

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCCGGAGAGGTGTCGTTAACAATG	ATTGAGTGCCGTCTGCCACTTGTAGA

Images

Construct Design Sequences

sgRNA sequence	TTTAGGAATGGATGCAAAACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTTTTGCCTTTGCCCCCAA	GCATATCCTTTCAGCTTGGCT	GGAATGGATGCAAAACCGGT	ATAGTCGCTTCGCAACAGCT

 

Amplicon 5'

Amplicon 3'

GTTTTTGCCTTTGCCCCCAAAGCTAAAAACCTTTCTTTTTGCTCTTTCTTTTGGGCTATCGCATGACCAAGTGGGTAATTACGGGAAGCAGAACAAAAAAACACGTTTGTAAACAGTTCATTCTGCTGGCGTTGTCCATTCGCCCGTTCTTCCCGCGGGTTCCACTGCTTCAGCGACCGGAAGCCGCTCAAGCGTGCTTCCCGTTCTGCGCAGGCGCATTCTCCTTGGTCACCCCAATTTTTTGCCATGACTCATGGGGTATGGCGTATTCAAATAAGAGAGGTGGAGATCTGATCGTAATGGTTGTTTTACTTGTTTTACATGCAAATTTTGACCAGAACATTGTCCAGAATTTCGGTTTTAACAATCTTCAGCTGTTGTGCTAATAACAATTTAGTGTTCTAGACTCTGCTTAATGGCTAGTTAATTGCTGCTCTGGTGCTGCGAGCGAATGATCGCCTCCAGGCTGCGTTCCGTGTGCTCTCCGCACTCGAAGCGATGGTAGTCGACGATCTCGCAGTTGTGCTCCTGCAAAGCCTCGCCCACCGTCTTGTCCGCATCGAGCAGATACTCCTGGTGAATCAGGCACGTCTCATCGTCCTTGTTCTCCGCCGGCTTGTCCTTGTCATATTCGCCGATCTTTGTCGGCTTCATGCCCACGATTTGCTGGCAAATGCTCTTGTGAAACTCAAAGTCCAGCAGGGGGTGCGTGGATCGGTAGGCTACGATCGCTCCGTACTTGCCCACCTGGGTGATGCCTTCCGTGGTGCCCACATTGGTGGGTGCGGGATGGGCGTAGCCCACCAGCTTCAGGTCGTTGTTAGCCTTAAAGCACAATGCGCGCCGGATGGTGGCATTCTCCCCGATGGCGCCGATCAGCAGAGCCAGGTGGTCGCCCAACGTGCGTCCCTCCTCCGTGCGCAAGTTTCTGAGGGCGTCCGCATCAAAGCCAAGCTGAAAGGATATGC

GGAATGGATGCAAAACCGGTTCTGATCCAAATATTGTGTACATTTCCCATAAAAATCTCGCTCACCTTCCACAGATCTCCATCGAAGTCCGTCAAGTCCGTGTATTGCAGACACATGCAGGCGACGTGGTCAACGAACCGCTTGAAGGTATCGTTCCTGGCTACGAAATCCGTCTCGCAATTCAGCTCGACCATGGCGCCCCGGTTTCCGCGGATGAGCACTCCAATCAGACCATGTGCAGTGGCTCGATCTGCGACCTTGGTGGCCTTCGACCAGCCGAGGGTCTGGGCCTGCTCGTGCAGCCACTTTTCAGCCTGCAAGAGCGAGATGATGGCAAGGTGAGTTGATTAGCACAGATGTGTTTTGATCACAAAGGAGTTTGCATGCGGTGCAGCTATATATATTACATAATAATATAGAAAAATCAGCAATTTTTCAAAATGTAGAGATCCCCATTTCGTGCTCTTTGTAATGGAAAGCTCACTGCACCGCTTGCAATAAAACTATTAGCCAACTACCCACCAATCCGACGTCGTTGTTGTGCTTCTCCAGCGCCTTCTTGCAATTGGCGAAGGTGTATCCGGTTTTCTTCCTCAGCGCCGCCAGGGCACTCTTCTCCAGGCCACCGGATCCGCCAGAGCCGGCATAAAGCTGCCGCGTGGAGTGAAGTGCGCGTGTGAAAAACCTTTGTAGCAGCATTTTTCCAAAAAATTTCCGAAATTTACTGGGAGGCCAAAATGATTTCTAGCGTGATTTGTGTGCGAATTGAATTGTTGCTCTTTGCCTGCTGTCAAGGGCAACTCGCCGGCTTGTTTGTTTTTCCGTTTTCCGACCTGTCGCTATGTAGTAGTTGCCCGGAAAATCAATTCATTCTAAACAACGGAAAAGCCTGTGTGAGATTTGCATTTGTTTTCAACTCCTTCGTATGTTCGCTTCGGTTCAAGAAAAATTAATCGCGTTGCGGCGCGAATAAATCCAAAGCGAGTGTGGTGCCTCGATAGGTGAACGACTGGTAAGCGTCGATAGTTTAGTTTTCGGCTTTTCTGTGTGCTTAATTCGAGCAATTAATTTTGAAACTGAAAATTTTGACAATTTTCCGTGTTGCACAATTCGGCTGTTAGGGATGGCAAAAAAGCCGGTAGGGCGCGTGTGCGGAGAACAGCTGTTGCGAAGCGACTAT

 

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