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CRIMIC CR00197

Gene CG2852

CG2852

Information

Donor	Location	Cytology	FBti	Stock number
pM36	2R:22645800..22645804	58F3	FBti0199699	79229

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CGTGAAGTCTAAGGATTGGCAGACATATGC	CAATTTCTCTTCGCTAGCCTCAGTCTG

Images

Construct Design Sequences

sgRNA sequence	GCACTTTGTAGGTTATAGCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTTTAGTCGGTGGCATCGG	TGAAGCAATTACGTGGCGTT	TGAGAGCCCCGCTATAACCT	GCTGCATGGAACGATGCATT

 

Amplicon 5'

Amplicon 3'

ACTTTAGTCGGTGGCATCGGCCTTGGCCACGGAGAAGGCCTCCGAAACGGGCAGGGTGCCGCTGTTGGCGATGACCACATCCTTGACGGGACGGTCGCGGGCATCAGTGGCCGAGTTCTCGATCTGGCGCACCACATTCATGCCCGACAGGATCTTGCCGAAGACGACGTGGCGTCCATCCAGCCAGCTGGTCTGCTTGGTGGTGATGAAGAACTGCGATCCGTTGGTGTCCTTGCCAGCGTTGGCCATGCTCAGCCAGCCGGCGCCATAGTGCTTCAGCTTGAAGTTCTCATCCTCGAAGCGCTCGCCGTAGATGGAGCGACCGCCGGTGCCGTCGCCCTTGGTGAAGTCACCGCCCTGGATCATGAAGTCCTTGATGATGCGGTGGAACTTGCTGCCCTTGTAGCTGCGGGGTATAAGAGCGTTGCATGATGGTCAGTTTTGTCAGCATGGGAATTTAAATTTAAAAAAAAACTGGGTTTAGAACTCGAAACCTAACCAAAAAAGAACATGCATACTTATAAATTCAGAGAAGAAAAAAGAAATACCCAAGAACTGCACTTTTATAGTATGGTAGACTTAATAACAGTTGGCGTTCTTTGTTAACCCATTAGTGCCGCTTTGTCAACTGATGTATGCGATTGGCCGCTCAAACGCCACGTAATTGCTTCA

TGAGAGCCCCGCTATAACCTACAAAGTGCCGCTGATTAGCGGCATCTTTTGCTGGCCTAGCGGTACCTGGCACCCTACTATGTTGGGGTATTTTCGGTACTCAAGTACCCTACTCACCCCTCGCCCTGCGGCTTCAGCGCCAGCTCCTTGAAGTTCTCCACCGTCTTGGGCACCGTCTTGCCGAACAGACCGATCTCGATGCGACCAGCGGGCTCGCCGCCAATGGTGATGTCAAAGAAAACCTACGCAATCGGAAGCGGTTTATTATTAACAAGTCATCTCACTTGATTTATGGCGCAGCAAATGTGTGTGTGTGTTTGTGTGTGTATGTACGTGGTGTATGCAGAATGTAAGGACGAAAGTCCAAGGAGCACGCACCTTCTCGGTCACTTTGGGACCCTTGCTATCGTCGGCAACAACGACGCCGGCCACCAGGGCTACCACGAAAACGGATAAGAACAGCTTCATCGTTCTAAATATTCACAGCGCTTCAGTTTATTCCTCAAGCGACGTCGCCAATGGATATTTCACGACTGAATTTTCGGTTTCTGTTAGTGATGTAAGAAAACTTTGTTGCATCGCCAATCGATGTTTTCCATCGTTTTTTATGTTTTTGTCGAAATATCGATGCTTAAGTGTGACCATTCGATGACGATGTCTGATGATCACACATTCGGGCGGAAAATTCAAATTCAAATAGTTAATACGCCGAAAGCATAAGTTAAGGTTTTCGTACGAAGGAACAAAGTAAGTTCGAAGAGTTGACTTTATTGGTATAGTTTTTAATATTTTAGGGATTAGAAGTCCAGTTTGCATTAGCACGTAGCATTCTCTCTTGTTTTATTGACGGTTCTGTACACAAAAAGTAAGTAAAAAAATATGCTTTTATTTATTGGTGTACTGCTATTTGCGTCTGAGATTTGTCCATATCCTTGGCTGCCTTCTCTGCTCTCTTCTTCTCCCTGCTGGCCAGAACTTTCTCCCTGGATTTCTGGGCCCTTTCAGCGCAATCCTTGGGTCGTGTCTTCAGGGTCATATCATCCACGGCCGCCTGTTTACGGGCTGATCTCGAAATGCATCGTTCCATGCAGC

 

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