CR00212

CRIMIC CR00212

Gene Top1

CG6146

Information

Donor	Location	Cytology	FBti	Stock number
pM36	X:15323793..15323813	13B6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ACGAGATGAACGACGACGAGGAGGAT	TGGGCACGGAACGCTGATGGTTACA

Images

Construct Design Sequences

sgRNA sequence	TCTAGACAAAGAAAAGAGGGTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCAGTGCGCATTGCTCAG	AGTCGCACCATCGAATTTGC	GACAAAGAAAAGAGGGTGGACC	GCGCCAGTTTGTCGATGAAG

Amplicon 5'	Amplicon 3'
GATCAGTGCGCATTGCTCAGATTCGTCCGAGCTCAAGGCAACATGCCGCCATGTGTTTTTGGCTGCGAGAAGTTTTCGGATGTCACTGACCTACATTTCCGAAAGTCACACACTCAGCGGACGGCCGACGCACAGTGGCTGCCGCAAGTTATTGTCCGCCTGTGGCTCGTCGCACAGTGGCGTTCGTTTGGGCGACAACTGTTGGCGCTCACTGTGCGGCGAGCGCCATTCGCCACGAAACGCATGCGTCGCTCCAGCCCCTGCTCCCCCCGCTCACCATGTACAGTTGCAATGAAAAAAAAAACAGCTGAGCAGGCCGAATAATGCGCATTTGAGGTTAGGTTCGAACGCGGCTGATTGACTGCTGCTTTGCGTAATGCTATAGATAAATCGGAGATATACCAAACCAAATGTTGCGCTCTTGGGTGGCATTGCGTCCATTCGAAAAGCAGAGTGTCTGCCTGGTGCTCGGACAGCGTATGTTGGCCGTAGCCACCGTTGCGGGCAAACGGAGAAGAGTGCGACGGAAGAGCGTCCAGGAAGAGCAAATTCGATGGTGCGACT	GACAAAGAAAAGAGGGTGGACCCAATTGCTCTTGATTACTGCTAGCAATGGCTAGCCTATGCCTATGCCTAGCCTATAATTTCTATAAAAACTAGAATAGTAATAGTACCCATTTATATTAGAAAGTTAATTGATAATATTTCTTCGACAATCGGGGGTGCTTAACATTTTGAACTCAACTATAGAAGGTTCAGTCTTAAAGCTCCTATTATCCGCGCCTGTATATGAAGCACTAACCCGAGTCATCCTACATTCCAGGTGGGAGGAGGAGAAGCGTGCCGATGGCGTCAAATGGTCAACGCTGGAGCACAAGGGACCCGTGTTTGCACCGCGATACGAACGGGTGCCACGCAATGTACGGTTCTACTACGATGGCAAGCCGTTGGAGCTCTCTGAGGAAACGGAGGAGGCAGCCACGTTCTATGCCAAGATGCTGAACCACGACTACTGCACCAAGGAAGTGTTCAATAACAACTTCTTCAAGGACTTCCGCAAGTCCATGACGCCCAGAGAGAGGGAGATTATCAAGGATTTCCGCAAGTGCAACTTCCAGGAGATGTTCAACTACTTCCAAGCGGAGTCCGAAAAGCGCAAGGCAGCCAGCAAGGAGGAGAAGCTGATCAAGAAGAACGAAAACGAGGCGCTGATGAAGGAATTCGGATTCTGCATGATTGATGGGCACAAGGAGAAGATCGGTAACTTCCGCCTGGAGCCGCCAGGCCTGTTCCGCGGTCGTGGCGAGCATCCCAAAATGGGCATGATCAAGCGGCGCATCCAAGCCAGCGATGTGTCCATTAACTGTGGCAAAGATTCAAAGGTGCCTTCACCGCCGCCCGGATCTCGCTGGAAGGAGGTGCGCCACGACAACACGGTCACCTGGCTGGCCTCCTGGATCGAGAATGTGCAGGGACAGGTCAAGTACATCATGTTGAATCCCTCCTCGAAACTCAAGGGCGAGAAGGATCACATTAAGTACGAGACGGCGCGACGCCTGGACAAGGTAATCGATAAGATACGTGCCACCTATCGCGACGAGTGGAAGTCCAAGGAGATGAGGGTTCGCCAGCGAGCGGTGGCCCTTTACTTCATCGACAAACTGGCGC

Amplicon 5'

Amplicon 3'

GATCAGTGCGCATTGCTCAGATTCGTCCGAGCTCAAGGCAACATGCCGCCATGTGTTTTTGGCTGCGAGAAGTTTTCGGATGTCACTGACCTACATTTCCGAAAGTCACACACTCAGCGGACGGCCGACGCACAGTGGCTGCCGCAAGTTATTGTCCGCCTGTGGCTCGTCGCACAGTGGCGTTCGTTTGGGCGACAACTGTTGGCGCTCACTGTGCGGCGAGCGCCATTCGCCACGAAACGCATGCGTCGCTCCAGCCCCTGCTCCCCCCGCTCACCATGTACAGTTGCAATGAAAAAAAAAACAGCTGAGCAGGCCGAATAATGCGCATTTGAGGTTAGGTTCGAACGCGGCTGATTGACTGCTGCTTTGCGTAATGCTATAGATAAATCGGAGATATACCAAACCAAATGTTGCGCTCTTGGGTGGCATTGCGTCCATTCGAAAAGCAGAGTGTCTGCCTGGTGCTCGGACAGCGTATGTTGGCCGTAGCCACCGTTGCGGGCAAACGGAGAAGAGTGCGACGGAAGAGCGTCCAGGAAGAGCAAATTCGATGGTGCGACT

GACAAAGAAAAGAGGGTGGACCCAATTGCTCTTGATTACTGCTAGCAATGGCTAGCCTATGCCTATGCCTAGCCTATAATTTCTATAAAAACTAGAATAGTAATAGTACCCATTTATATTAGAAAGTTAATTGATAATATTTCTTCGACAATCGGGGGTGCTTAACATTTTGAACTCAACTATAGAAGGTTCAGTCTTAAAGCTCCTATTATCCGCGCCTGTATATGAAGCACTAACCCGAGTCATCCTACATTCCAGGTGGGAGGAGGAGAAGCGTGCCGATGGCGTCAAATGGTCAACGCTGGAGCACAAGGGACCCGTGTTTGCACCGCGATACGAACGGGTGCCACGCAATGTACGGTTCTACTACGATGGCAAGCCGTTGGAGCTCTCTGAGGAAACGGAGGAGGCAGCCACGTTCTATGCCAAGATGCTGAACCACGACTACTGCACCAAGGAAGTGTTCAATAACAACTTCTTCAAGGACTTCCGCAAGTCCATGACGCCCAGAGAGAGGGAGATTATCAAGGATTTCCGCAAGTGCAACTTCCAGGAGATGTTCAACTACTTCCAAGCGGAGTCCGAAAAGCGCAAGGCAGCCAGCAAGGAGGAGAAGCTGATCAAGAAGAACGAAAACGAGGCGCTGATGAAGGAATTCGGATTCTGCATGATTGATGGGCACAAGGAGAAGATCGGTAACTTCCGCCTGGAGCCGCCAGGCCTGTTCCGCGGTCGTGGCGAGCATCCCAAAATGGGCATGATCAAGCGGCGCATCCAAGCCAGCGATGTGTCCATTAACTGTGGCAAAGATTCAAAGGTGCCTTCACCGCCGCCCGGATCTCGCTGGAAGGAGGTGCGCCACGACAACACGGTCACCTGGCTGGCCTCCTGGATCGAGAATGTGCAGGGACAGGTCAAGTACATCATGTTGAATCCCTCCTCGAAACTCAAGGGCGAGAAGGATCACATTAAGTACGAGACGGCGCGACGCCTGGACAAGGTAATCGATAAGATACGTGCCACCTATCGCGACGAGTGGAAGTCCAAGGAGATGAGGGTTCGCCAGCGAGCGGTGGCCCTTTACTTCATCGACAAACTGGCGC

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